EDL muscles (n indicated in figure legends) were excised from deeply anesthetized mice with 3% isoflurane, hung from a computer-controlled servomotor (300B, Aurora Scientific, Inc), and mounted in a heated (30 °C) oxygenated tissue bath containing a physiologic salt solution as described [59 (link), 60 (link)]. Experiments were performed, and data were analyzed using DMA software (Aurora Scientific, Inc) and Prism 7 (Graphpad), as previously described (Treat-NMD SOP, DMD_M.1.2.002, and [60 (link)]). Significance for twitch and tetanus was calculated by one-way ANOVA, and for the force frequency analysis, it was calculated by two-way ANOVA using Prism 7 (GraphPad).
Dma software
Manufactured by Aurora Scientific
The DMA software is a specialized program used for the analysis and interpretation of data obtained from dynamic mechanical analysis (DMA) instruments. It provides the core functionality for processing and visualizing the results of DMA experiments.
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Lab products found in correlation
5 protocols using dma software
1
Measuring Contractile Function of Excised EDL Muscles
2
Isolated Extensor Digitorum Longus Muscle Analysis
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Extensor digitorum longus (EDL) muscles were dissected from deeply anesthetized mice with 2.5% isoflurane and mounted between two platinum electrodes, clamped at one tendon, and attached at the other tendon to a force transducer placed in an oxygenated bath containing a physiologic salt solution (PSS buffer, pH 7.6) at 30°C. Experiments were performed using the isolated muscle test system for mice (Aurora Scientific) as described previously (https://treat-nmd.org/wp-content/uploads/2016/08/cmd-DMD_M.1.2.002.pdf ; Sperringer and Grange, 2016 (link)). Data were analyzed using DMA software (Aurora Scientific), and the force was normalized by the EDL muscle weight.
3
In Vitro Contractile Function Assessment
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A fibre bundle from the diaphragm was isolated to allow in vitro contractile function to be assessed using a length‐controlled lever system (301B, Aurora Scientific Inc., Aurora, Canada), as previously described.19 , 22 Briefly, a muscle bundle was mounted vertically in a buffer‐filled organ bath (~22 °C), set at optimal length, and after 15 min was stimulated via a force–frequency protocol using 1–300 Hz (600 mA, 500 ms train duration, and 0.25 ms pulse width). The muscle was then subjected to a force–velocity protocol where it was allowed to shorten against external loads (80–10% of the maximal tetanic force; each separated by 1 min) after being stimulated at 150 Hz for 300 ms. Shortening velocity was determined 10 ms after the first change in length and on the linear section of the transient (DMA software, Aurora Scientific Inc.). Force (N) was normalized to muscle cross‐sectional area (CSA; cm2) by dividing muscle mass (g) by the product of Lo (cm) and estimated muscle density (1.06), which allowed specific force in N/cm2 to be calculated. Shortening velocity was normalized to optimal muscle length (in Lo/s), while power was calculated for each load as the product of shortening velocity and specific force (in W/cm2).
4
Evaluating In Vivo Muscle Force
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The 1300 A 3-in-1 whole animal system (Aurora Scientific) was used for in vivo muscle force analysis. Mice were first anesthetized and kept warm by heat lamp. The maximum twitch and tetanic force were evaluated according to manufacturer’s instruction. Five repetitive tests were performed for each limb and DMA software (Aurora scientific) was used for results analysis.
5
In Vivo Muscle Contractile Force Analysis
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In vivo TA muscle force analysis was performed with 1300 A 3-in-1 whole animal system (Aurora Scientific) as previously described60 (link)–62 (link). Mice were anesthetized by 3-bromo-2-fluoro-phenyl methanol (31.2 g/kg body weight) injection and kept warm by heat lamp. The hind limb was shaved and immobilized by fixing the leg in a frame without disturbing the blood flow. Tie around the patella ligament of the distal TA muscle using a bread silk suture. Make an incision to expose the sciatic nerve and tie another knot around the proximal end of the sciatic nerve. Attach the distal TA tendon suture loop to the lever arm hook of the instrument and measure the contractile force. During the process, TA muscle was constantly superfused with pre-warmed 37 °C Ringer’s buffer (118 mM NaCl, 4.75 mM KCl, 1.18 mM KH2PO4, 1.18 mM MgSO4, 2.5 mM CaCl2, 25 mM NaHCO3, and 11 mM glucose). The results were analyzed by DMA software (Aurora scientific). For each treatment, five independent experiments were performed. The identity of each mouse was blinded to the personal who operated the measurement.
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