Lc ms grade organic solvents

The DON standard, LC/MS-grade organic solvents and other reagents were purchased from Sigma-Aldrich (Steinheim, Germany). The distillated water used for the studies was purified using a Milli-Q system (Millipore, Bedford, MA, USA). A DON stock solution was prepared in methanol, at a concentration of 1 mg/mL, and was stored at −20 °C. Three buffer solutions, based on citrate (pH 3 and 6) and ammonia (pH 9), were used in the experiment.

Each fungal rice culture for the presence of mycotoxins was extracted and purified according to the method described by Urbaniak et al [37 (link)]. The liquid cultures from day 8 were subjected to mycotoxin quantification. The samples were collected from biological triplicates and were subjected to mycotoxin quantification.
Mycotoxin standards of high purity (fumonisins B₁, B₂, B₃, enniatins A, A₁, B, B₁, beauvericin and moniliformin) were purchased from Sigma-Aldrich (Steinheim, Germany). Standard solutions of mycotoxins were prepared in methanol and kept in a freezer at −20 °C. LC/MS-grade organic solvents, water and other reagents were purchased from Sigma-Aldrich (Steinheim, Germany). Qualitative and quantitative analysis of mycotoxins were performed using a LC–PDA/TQD system consisting of an UPLC™ system (Acquity, Waters, Milford, MA, USA) coupled to a photodiode array detector (PDA) and a triple quadrupole mass spectrometer (TQD; Waters Micromass, Manchester, UK) according to the methods described in detail earlier [37 (link),49 (link)]

AH-1 was synthesized as previously described^{19 (link)}. D6-25-hydroxyvitamin D₃ (26,26,26,27,27,27-D6, d6-25(OH)D₃) and LCMS grade organic solvents were purchased from Sigma-Aldrich (St. Louis, MO, USA). 4-[2-(6,7-dimethoxy-4-methyl-3-oxo-3,4-dihydroquinoxalyl)ethyl]-1,2,4-triazoline-3,5-dione (DMEQ-TAD) was purchased from FUJIFILM Wako Pure Chemicals (Osaka, Japan). Authentic standards of 24R,25(OH)₂D₃, and 24-oxo-25(OH)D₃ were prepared as previously described^{25 (link)}. Other chemicals were commercially available and of the highest quality.