Human laryngeal carcinoma cells Hep-2 were obtained from BIOBAIYE (Shanghai China). Hep-2 cells were routinely cultured in DMEM (Gibco), which consisted of 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Solarbio) at 37 °C. Placed within an incubator of 5% CO2, the cells were digested with 0.25% membrane protease (Sigma) every 2–3 days.
Membrane protease
Manufactured by Merck Group
Membrane protease is a laboratory equipment used for the analysis and study of membrane-bound proteins. It is designed to isolate and purify membrane proteins from various biological samples. The core function of this product is to facilitate the extraction and separation of membrane proteins, enabling researchers to study their structure, function, and interactions in a controlled environment.
Automatically generated - may contain errors
Lab products found in correlation
Hep 2, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Tu686, by BNCC (1 mentions)
Tu177, by BNCC (1 mentions)
Amc hn 8, by BNCC (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using membrane protease
1
Culturing Human Laryngeal Carcinoma Hep-2 Cells
2
Culturing Human Laryngeal Cancer Cell Lines
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The human laryngeal cancer cell lines (ie TU686, TU177, AMC‐HN‐8, and LSC‐1) (Bena culture collection) and the normal human bronchial epithelial cells (NHBEC) were routinely cultured in RPMI1640 medium (Gibco) that consisted of 10% fetal bovine serum (FBS, Hyclone) at 37°C. Placed within an incubator of 5% CO2, the cells were digested with 0.25% membrane protease (Sigma) every 2‐3 days.
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