Ampk ser485

Manufactured by Cell Signaling Technology

Sourced in United States

AMPK (Ser485) is a lab equipment product offered by Cell Signaling Technology. It is an antibody that specifically recognizes the phosphorylation of AMPK at serine 485. AMPK is an important cellular energy sensor that plays a crucial role in regulating cellular metabolism.

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Lab products found in correlation

Occludin, by Bioss Antibodies (1 mentions) Bs 0295 hrp, by Bioss Antibodies (1 mentions) Sirt3, by Proteintech (1 mentions) β actin, by Bioss Antibodies (1 mentions) Il 1β, by Cell Signaling Technology (1 mentions) Glut4, by Cell Signaling Technology (1 mentions) Estrogen receptor alpha, by Abcam (1 mentions)

2 protocols using ampk ser485

Western Blot Analysis of Tight Junction Proteins and Signaling Pathways

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After washing in ice-cold PBS, the acquired tissues and cells were processed with ice-cold radioimmunoprecipitation assay (RIPA) lysis buffer complemented with phenylmethylsulfonyl fluoride (PMSF, at 1 mM), followed by Western blotting in accordance with the previously mentioned methods [28 (link)], with Occludin (diluted at 1 : 1000; bs-10011R; Bioss), ZO-1 (dilution rate 1 : 1000; bs-1329R; Bioss), p-AMPK (Ser485) (diluted at 1 : 1000; #2537; Cell Signaling Technology; Danvers; USA), Claudin-11 (dilution rate 1 : 1000; bs-21509R; Bioss), AMPK (Ser485) (1 : 1000 dilution; #2532; Cell Signaling Technology), SIRT3 (diluted at 1 : 1000; 10099-1-AP; Proteintech), β-actin (1 : 3,000 dilution; bs-0061R; Bioss), SOD2 (dilution rate 1 : 1000; bs-23402R; Bioss), NLRP3 (1 : 1000 dilution; 19771-1-AP; Proteintech), and IL-1β (dilution rate 1 : 1000; #12703; Cell Signaling Technology) as the primary antibodies, as well as the HRP-labeled goat anti-rabbit antibody (diluted at 1 : 3000, bs-0295-HRP; Bioss) as the secondary antibody. Ultimately, the ECL solution was applied to measure the bands, and the Image J software was employed to determine the signals.

Xiao L., Fang Z., Wang Q., Sheng X., Qi X., Xing K., Guo Y., Ni H., Wang X, & Zhang Y. (2023). Curcumin Ameliorates Age-Induced Tight Junction Impaired in Porcine Sertoli Cells by Inactivating the NLRP3 Inflammasome through the AMPK/SIRT3/SOD2/mtROS Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2023, 1708251.

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Western Blot Analysis of Metabolic Proteins

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Protein content was measured as previously described (Winn, et al. 2017b (link)). Briefly, protein samples (10
μg/lane were separated by SDS-PAGE, transferred to polyvinylidene
difluoride membranes, and probed with primary antibodies, including UCP1
(#U6382, 1:1000; Sigma-Aldrich), UCP2 (#89326, 1:1000; Cell Signaling), Acetyl
Co-A Carboxylase or ACC (#3662, 1:1000, Cell Signaling), Phosphorylated Acc or
PACC(#3661, 1:1000 Cell Signaling), OXPHOS (#M5604, 1:1000, MitoSciences), Ampk
(#2532, 1:1000, Cell Signaling), AMPK-SER485(4185, 1:1000, Cell Signaling),
AMPK-THR172 (#2531, 1:1000, Cell Signaling), Estrogen Receptor Alpha (Yasrebi,
et al.)(#75635, 1:1000, Abcam), Estrogen Receptor Beta (ERB) (#AB3577, 1:2000,
Abcam), Glut4 (#2213, 1:1000, Cell Signaling), Akt (#4691, 1:500, Cell
Signaling). Intensity of individual protein bands were quantified using
FluoroChem HD2 (AlphaView, version 3.4.0.0), and expressed as ratio to Amide
Black total protein stain.

Clookey S.L., Welly R.J., Zidon T.M., Gastecki M.L., Woodford M.L., Grunewald Z.I., Winn N.C., Eaton D., Karasseva N.G., Sacks H.S., Padilla J, & Vieira-Potter V.J. (2018). Increased Susceptibility to OVX-Associated Metabolic Dysfunction in UCP1 Null Mice. The Journal of endocrinology.

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