Mbs7604494

Immunohistochemistry was performed using a Rabbit specific HRP/DAB (ABC) Kit (ab64261, Abcam, Cambridge, UK) and anti-ST2 antibody (MBS7604494, MyBioSource, San Diego, CA, USA). Briefly, the sections were brought to boiling temperature in 1× citrate buffer (ab93678, Abcam, Cambridge, UK), pH 6.0, and maintained at a sub-boiling temperature for 10 min for antigen retrieval. Sections were incubated with a blocking solution and incubated overnight at 4 °C with an anti-ST2 antibody (1:500). The sections were subsequently incubated with a biotinylated goat anti-rabbit IgG secondary antibody. Staining was developed using DAB solution for 1 min (1:50; Millipore, Billerica, MA, USA). The ST2-positive area was measured using the Fiji application.

All immunohistochemistry processes were performed as described previously [40 (link)]. Briefly, immunohistochemistry was conducted using a mouse-specific HRP/DAB (ABC) Kit with growth stimulation-expressed gene 2 (ST2) antibody (MBS7604494, Mybiosource, San Diego, CA, USA) and E-cadherin (#14472, Cell Signaling Technology, Danvers, MA, USA). Tissue sections were boiled and incubated at 4 °C with anti-ST2 and E-cadherin antibodies with a blocking solution. Sections were then exposed to biotinylated goat anti-rabbit IgG secondary antibody and DAB solution (Millipore, Billerica, MA, USA) was used for antigen labeling.