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Grp78

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GRP78 is a molecular chaperone protein that plays a crucial role in the endoplasmic reticulum (ER) stress response. It facilitates the proper folding and assembly of proteins within the ER, helping to maintain cellular homeostasis.

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Lab products found in correlation

Bcl2 associated x (bax), by Affinity Biosciences (2 mentions) Caspase12, by Affinity Biosciences (2 mentions) Bcl 2, by Affinity Biosciences (2 mentions) Caspase 9, by Affinity Biosciences (2 mentions) Caspase 3, by Affinity Biosciences (2 mentions) α sma, by Abcam (1 mentions) Sds page, by Ipsen (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Solarbio (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) P erk, by Affinity Biosciences (1 mentions) Caspase8, by Affinity Biosciences (1 mentions) P jnk, by Affinity Biosciences (1 mentions) Lectins, by Vector Laboratories (1 mentions) Cleaved caspase 3, by Affinity Biosciences (1 mentions) β actin, by Affinity Biosciences (1 mentions) Gapdh, by Affinity Biosciences (1 mentions) Ha tag, by ABclonal (1 mentions) Ecl solution system, by Sparkjade (1 mentions) Trizol kit, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Bio-Rad (1 mentions)

Spelling variants of this product

Anti-GRP78 (4 citations)

4 protocols using grp78

1

Western Blotting of Lacrimal Gland Proteins

Cited 1 time
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Lacrimal gland tissue was extracted with Radioimmunoprecipitation Assay (RIPA) Lysis Buffer (Beyotime) mixed with phenylmethylsulfonyl fluoride (PMSF; Solarbio). Protein extracts were resolved via 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE; EPizyme, Shanghai, China) and then electrotransferred to a polyvinylidene difluoride membrane. After blocking, the membranes were probed with primary antibodies to AQP5 (1:2000, Abcam), α-SMA (1:2000, Abcam), GAPDH (1:3000, Kangchen, Shanghai, China), CHOP (1:500, Affinity Biosciences, Wuhan, China), Bax (1:1000, Affinity Biosciences), Bcl-2 (1:2000, Affinity Biosciences), Caspase12 (1:1000, Affinity Biosciences), and GRP78 (1:1000, Affinity Biosciences). Horseradish peroxidase–conjugated secondary antibodies were used, and the bands were developed using a Western Bright Electrochemiluminescence (ECL) substrate (Applygen, Beijing, China). GAPDH was used for normalization of expression [34 (link)].
Hu S., Di G., Cao X., Liu Y., Wang Y., Zhao H., Wang D, & Chen P. (2021). Lacrimal gland homeostasis is maintained by the AQP5 pathway by attenuating endoplasmic reticulum stress inflammation in the lacrimal gland of AQP5 knockout mice. Molecular Vision, 27, 679-690.
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2

Antibody Procurement for Cell Signaling

Cited 2 times
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Rabbit polyclone anti-CHOP, cytochrome c (Cyto C), ATF6, PERK, GRP78, ARF4, Bax, Bak, caspase3, cleaved caspase 3, caspase 8, caspase 9, IRE1, DR4, DR5, P-JNK, Bcl-2, Fas, β-Actin, Gapdh, and Tubullin were obtained from Affinity Biosciences (Shanghai, China). Mouse monoclonal anti-Lewis a (Lea), Lewis b (Leb), and Lewis y (Ley) were purchased from Abcam (Cambridgeshire, UK) [74 (link)]. Mouse monoclonal anti-SLex was purchased from BD Biosciences (San Diego, CA, USA) [75 (link)]. FITC-conjugated anti-mouse/rabbit IgG was purchased from Affinity Biosciences (Shanghai, China). Goat anti-rabbit IgG-HRP was obtained from Life Science. E-selectin and P-selectin were purchased from R&D System (Minneapolis, MN, USA). Lectins were purchased from Vector Laboratories (Burlingame, CA, USA). Cells were cultured with cell growth medium (Gibco, Shanghai, China) containing fetal bovine serum (FBS) (ABW, Shanghai, China) and penicillin/streptomycin (Gibco, Shanghai, China).
Luan X., Sun M., Zhao X., Wang J., Han Y, & Gao Y. (2022). Bisimidazolium Salt Glycosyltransferase Inhibitors Suppress Hepatocellular Carcinoma Progression In Vitro and In Vivo. Pharmaceuticals, 15(6), 716.
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3

Western Blot Analysis of NRVM Proteins

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NRVMs or homogenized heart tissues were lysed. Lysates were separated by 8–15% SDS-PAGE gels and then electro-transferred to nitrocellulose membranes, subsequently blocked with 5% non-fat milk-TBST buffer for 1 h at room temperature. The membranes were incubated with primary antibodies at 4 °C overnight. Then the membranes were incubated with horseradish peroxidase-conjugated secondary antibody (1:5000) for 1h at room temperature. The target bands were detected by the ECL solution system (Shandong Sparkjade Biotechnology Co., Ltd., Shandong, China) and analyzed using Image J. LC3 (2775s), P62 (39749s), PERK (3192s), p-PERK (3179s), eIF2α (5324s), p-eIF2α (3398s), ATF4 (11815s) were obtained from Cell Signaling Technology; VEGF-B (ab110649), GRP78 (ab21685) were purchased from Abcam; β-actin (sc47778), VEGF-B (sc-80442, 1:200 for IP) were purchased from Santa Cruz Biotechnology; Flag-tag (390002), HA-tag (301113) were obtained from ZENBIO; GST-tag (T0007), Flag-tag (T0053, 1:100 for IP) were purchased from Affinity; GRP78 (66574–1-Ig, 1:50 for IP) was purchased from Proteintech, HA-tag (AE008, 1:100 for IP) was purchased from Abclonal.
Zhang S., Tian W., Duan X., Zhang Q., Cao L., Liu C., Li G., Wang Z., Zhang J., Li J., Yang L., Gao Y., Xu Y., Liu J., Yan J., Cui J., Feng L., Liu C., Shen Y, & Qi Z. (2024). Melatonin attenuates diabetic cardiomyopathy by increasing autophagy of cardiomyocytes via regulation of VEGF-B/GRP78/PERK signaling pathway. Cardiovascular Diabetology, 23, 19.
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4

Aβ25-35-Induced Apoptosis Pathway Analysis

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Aβ25-35 was purchased from Sigma-Aldrich (A4559, St Louis, MO, USA). Donepezil Hydrochloride Tablet (Don) was purchased from Eisai, Shanghai, China. HPTQC (Z20080006) was provided by the First Affiliated Hospital of Anhui University of Chinese Medicine. Annexin V-PI kit (Lot.600125a) was purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China). Protein lysate (Lot. 70126373), PMSF (Lot. 329-98-6), Tween-20 (Lot. 20CS180) and Tris (Lot. 1130K07) were purchased from Beijing Solarbio Science & Technology Co., Ltd. The following antibodies, Caspase3 (Lot. 89Z0306), Caspase9 (Lot. 74z1125), Caspase12 (Lot. 76r9498), CHOP (Lot. 37s3359), and GRP78 (Lot.64f2874), were from Affinity Biosciences (Cincinnati, OH, USA). PVDF membrane (Lot. RB88250) was purchased from Millipore. TRIzol kit (Lot. 15596-026) was purchased from Ambion (Carlsbad, CA, USA). FBS (Lot.10099140C) was purchased from Bio-Rad Laboratories.
Li X., Qin Y., Ye S., Song H., Zhou P., Cai B, & Wang Y. (2022). Protective effect of Huangpu Tongqiao capsule against Alzheimer's disease through inhibiting the apoptosis pathway mediated by endoplasmic reticulum stress in vitro and in vivo. Saudi Pharmaceutical Journal : SPJ, 30(11), 1561-1571.
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