Polyclonal rabbit anti occludin

d-glucose, l-glucose and insulin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sdc1 polyclonal antibody (sc-7099) and HPSE monoclonal antibody (mAb, sc-25826) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and soluble Sdc1 ELISA kits and heparanase ELISA kits were purchased from Diaclone (Besançon, France) and Cusabio (Wuhan, China) respectively. Rabbit polyclonal anti-occludin and anti-ZO-1 antibodies were obtained from Invitrogen (Cambridge, MA, USA). MAPK and phospho-MAPK family antibody sampler kits (#9926, #9910) were purchased from Cell Signaling Technology (Beverly, MA, USA); SB203580 (a p38 MAPK inhibitor) and Anisomycin (a p38 MAPK activator) were purchased from Calbiochem (San Diego, CA, USA) and Sigma-Aldrich respectively. Horseradish peroxidase (HRP)-conjugated anti-rabbit IgG, and anti-β-actin antibodies were purchased from Zhongshan Goldenbridge Biotechnology (Beijing, China). Alexa Fluor-594-conjugated secondary antibody was obtained from Invitrogen.

Primary and secondary antibodies were used at the following dilutions: Mouse monoclonal anti- cytokeratin 7 (Dako, Agilent technologies, CA, USA, Cat. No. M7018), 1:20; Mouse monoclonal anti-E-cadherin (BD Transduction Laboratories, San Jose, CA, USA; Cat. No. 610182), 1:30; Rabbit polyclonal anti-N-cadherin (Abcam, Cambridge, MA, USA; Cat. No. ab18203), 1:100; Rabbit polyclonal anti-occludin (Invitrogen, Thermo Fisher Scientific, San Francisco, CA, USA, Cat. No. 71-1500), 1:30; Mouse monoclonal anti-claudin-2 (Zymed Laboratories, Thermo Fisher Scientific, Cat. No. 32-5600), 1:100; Rabbit polyclonal anti-claudin-4 (Zymed Laboratories, Thermo Fisher, Cat. No. 32-9400), 1:100; Alexa-Fluorophore-conjugated anti-mouse and anti-rabbit secondary antibodies (Invitrogen, Molecular Probes, Leiden, The Netherlands), 1:400.