5977a inert mass detector

GC–MS analyses were carried out with a 7890B gas chromatograph connected to a 5977A inert mass detector (Agilent) fitted with a HP5-MS fused silica capillary column (30 m, 0.25 mm i. d., 0.50 μm film). Instrumental parameters were (1) inlet pressure, 77.1 kPa, He 23.3 mL min⁻¹, (2) injection volume, 2 μL, (3) transfer line, 250 °C, and (4) electron energy 70 eV. The GC was programmed as follows: 5 min at 50 °C increasing at 5 °C min⁻¹ to 320 °C, and operated in split mode (10:1, 60 s valve time). The carrier gas was He at 1 mL min⁻¹. Retention indices (I) were determined from a homologous series of n-alkanes (C₇−C₄₀). Compound identification of the side products of terpene cyclases was based on a matching mass spectrum to a library mass spectrum and retention index to published data.
HRMS analyses were carried out with a 7890B gas chromatograph connected to a 7200 accurate-mass Q-TOF mass detector (Agilent) eqipped with a HP5-MS fused silica capillary column (30 m, 0.25 mm i. d., 0.50 μm film). Instrumental parameters were (1) inlet pressure, 83.2 kPa, He 24.6 mL min⁻¹, (2) injection volume, 2 μL, (3) transfer line, 250 °C, and (4) electron energy 70 eV. The GC was programmed for HRMS as follows: 5 min at 50 °C increasing at 5 °C min⁻¹ to 320 °C, and operated in split mode (50:1, 60 s valve time). The carrier gas was He at 1 mL min⁻¹.

GC–MS analyses were performed using a 7890B gas chromatograph connected to a 5977A inert mass detector (Agilent). The GC was equipped with a HP5-MS fused silica capillary column (30 m, 0.25 mm i. d., 0.50 μm film). The instrumental parameters of the GC were (1) inlet pressure: 77.1 kPa, He 23.3 mL min⁻¹, (2) injection volume: 2 μL, (3) split mode (10:1 to 50:1, 60 s valve time), (4) carrier gas: He 1 mL min⁻¹, (5) transfer line: 250 °C, and (6) electron energy: 70 eV. The temperature program of GC was set to: 5 min at 50 °C, then increasing by 10 °C min⁻¹ to 320 °C, followed by 5 min at 320 °C.
GC–HRMS analyses were carried out with a 7890B gas chromatograph connected to a 7200 accurate mass QTOF mass detector (Agilent) equipped with a HP5-MS fused silica capillary column (30 m, 0.25 mm i. d., 0.50 μm film). The instrumental parameters were (1) inlet pressure: 83.2 kPa, He 24.6 mL min⁻¹, (2) injection volume: 2 μL, (3) split mode (10:1 to 50:1, 60 s valve time), (4) carrier gas: He 1 mL min⁻¹, (5) transfer line: 250 °C, (6) electron energy 70 eV, (7) collision cell gas flow: 1 mL min⁻¹ N₂, (8) collision energy: 15 V, and (9) MS 1 scan resolution mode: narrow (m/z +/− 0.5). The temperature program of GC was set to: 5 min at 50 °C, then increasing by 10 °C min⁻¹ to 320 °C, followed by 5 min at 320 °C.