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Dopamine hcl

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Dopamine-HCl is a chemical compound used in laboratory and research settings. It serves as a precursor for the neurotransmitter dopamine and is utilized for various analytical and experimental purposes. The core function of Dopamine-HCl is to provide a reliable source of the dopamine molecule for scientific investigation and analysis.

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Lab products found in correlation

Quinpirole, by Bio-Techne (3 mentions) Serotonin creatinine sulfate monohydrate, by Merck Group (2 mentions) Dibac4 3, by AnaSpec (2 mentions) Sulpiride, by Merck Group (2 mentions) Nitric acid, by Thermo Fisher Scientific (2 mentions) Glutaraldehyde, by Thermo Fisher Scientific (2 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions) Silver nitrate, by Merck Group (2 mentions) Cell culture reagents, by Thermo Fisher Scientific (2 mentions) Rhodamine 123 ra123, by Merck Group (2 mentions) Rhodamine b base rab, by Merck Group (2 mentions) Neutral red dye, by Avantor (2 mentions) Barnstead ultrapure water purification system, by Thermo Fisher Scientific (2 mentions) Amicon ultra centrifugal filter, by Merck Group (2 mentions) Nih 3t3 fibroblasts, by American Type Culture Collection (2 mentions) Dl metanephrine hcl, by Merck Group (2 mentions) Dl normetanephrine hcl, by Merck Group (2 mentions) L dopa d3, by Merck Group (2 mentions) Stable deuterated isotopesfor dopamine d4 hcl, by CDN Isotopes (2 mentions) Norepinephrine d6 hcl, by CDN Isotopes (2 mentions)

26 protocols using dopamine hcl

1

Dopamine, Serotonin, and DiBAC4(3) Assays

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Dopamine HCl and serotonin creatinine sulfate monohydrate were obtained from Sigma-Aldrich. Just prior to use, serotonin was dissolved in artificial sea water (ASW, Instant Ocean Aquarium Systems) and dopamine was dissolved in ASW containing 10 mg% ascorbic acid (ASWA) buffered with sodium bicarbonate (pH 7.0) to retard dopamine oxidation [18 (link)]. The fluorescent probe DiBAC4(3) was obtained from AnaSpec, Inc., and stock and working solutions were stored in brown bottles. DiBAC4(3) stock solutions (24 mM) were dissolved in DMSO and working solutions (2.4 μM) were freshly prepared in ASW. All other chemicals were obtained from Fisher Scientific.
Catapane E.J., Nelson M., Adams T, & Carroll M.A. (2016). Innervation of Gill Lateral Cells in the Bivalve Mollusc Crassostrea virginica Affects Cellular Membrane Potential and Cilia Activity. Journal of pharmacological reports, 1(2), 109.
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2

Dopamine-Functionalized Silica Nanoparticles

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Tannic acid and dopamine·HCl were purchased from Sigma-Aldrich. Tetraethoxysilane (TEOS), ammonium hydroxide, Cu(NO3)2·3H2O, Fe(NO3)3·9H2O, Cd(NO3)2·4H2O, Mg(NO3)2·6H2O and Cr(NO3)3·9H2O were purchased from Meryer, China. Ethanol was purchased from Sinopharm Chemical Reagent Co., Ltd. A standard stock solution (1 g/L) of Cu2+ was prepared by dissolving Cu(NO3)2·3H2O in deionized water. The desired Cu2+ solutions (10~100 mg L−1) were prepared by diluting the standard stock solution. All other reagents were of AR grade and used as received.
Gao J., Lei H., Han Z., Shi Q., Chen Y, & Jiang Y. (2017). Dopamine functionalized tannic-acid-templated mesoporous silica nanoparticles as a new sorbent for the efficient removal of Cu2+ from aqueous solution. Scientific Reports, 7, 45215.
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3

Electrochemical Cocaine Aptasensor Fabrication

Cited 2 times
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Copper(I) Bromide (CuBr 98%), imidazole (≥99%), bromoisobutyryl bromide (BIBB 98%), [2-(methacryloyloxy) ethyl]-dimethyl-(3-sulfopropyl) ammonium hydroxide (SBMA 97%), 2,2′-bipyridine (BPY, ≥99%), tetrahydrofluran (THF), tert-butyl chlorodimethylsilane (TBDMS, 97%), triethylamine (TEA), tetrabutylammonium fluoride (TBAF), and dopamine-HCl (99%) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Cocaine-targeting aptamer, 5′-HS-(CH2)6-AGACAAGGAAAATCCTTCAATGAAGTGGGTCG-(CH2)7-MB-3′ [35 (link),37 (link),45 (link)], was designed with a 5′ terminal thiol group (attached with a six-carbon linker) to facilitate direct covalent linkage to the gold surface and a 3′ terminal methylene blue (MB) group (attached with a seven-carbon linker) as an electrochemical reporter molecule. Aptamer was synthesized and dual HPLC purified prior to purchase from Integrated DNA Technologies, Inc. (Coralville, IA, USA). This specific cocaine-targeting aptamer has been well characterized to exhibit a 90 mM Kd [37 (link)]. All DI water and PBS (1×) used are DNase free.
Wu B., Castagnola E, & Cui X.T. (2023). Zwitterionic Polymer Coated and Aptamer Functionalized Flexible Micro-Electrode Arrays for In Vivo Cocaine Sensing and Electrophysiology. Micromachines, 14(2), 323.
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4

Opioid and Cocaine Receptor Ligands

Cited 1 time
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17-Cyclopropylmethyl-3-hydroxy-4,5α-epoxy-7,8-en-6-β-[(3′-iodo) benzamido]-morphinan (MP1104) and Naltrindole (NTI) (with > 98% purity) (Váradi et al., 2015 (link)) were provided by Dr. Susruta Majumdar (Memorial Sloan Kettering Cancer Centre, New York, USA). Trans-3,4-dichloro-N-methyl-N-(2–1-pyrrolidinyl)-cyclohexyl-benzeacetamide (U50,488) (Sigma-Aldrich, Auckland, New Zealand) and yohimbine (In Vitro Technologies Ltd., Auckland, NZ) were dissolved in dimethyl sulfoxide (DMSO)/Tween-80/water (Milli-Q) in a ratio of 2:1:7. All drugs were administered via intraperitoneal (i.p.) injections except NTI which was injected subcutaneously (s.c.). Cocaine HCl (BDH Ltd., Wellington, NZ) was dissolved in heparinised saline (3.0 U/mL) for intravenous (i.v) infusions and in physiological saline for i.p. injections. The KOPr antagonist nor-BNI (In Vitro Technologies) was dissolved in physiological saline and injected s.c. at a volume of 1 mL/kg 24h prior to experimental testing. Dopamine-HCl (Sigma-Aldrich) was dissolved in Milli-Q water. All drugs weights refer to the salt.
Atigari D.V., Uprety R., Pasternak G.W., Majumdar S, & Kivell B.M. (2019). MP1104, a mixed kappa-delta opioid receptor agonist has anti-cocaine properties with reduced side-effects in rats. Neuropharmacology, 150, 217-228.
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5

Cocaine and Dopamine Receptor Assays

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Cocaine-HCl was obtained from the National Institute on Drug Abuse. Dihydro-β-erythroidine hydrobromide (DHBE), nomifensine, quinpirole-HCl, and SCH23390 were purchased from Tocris Bioscience. Dopamine-HCl was purchased from Sigma-Aldrich. All drugs were dissolved in ACSF. All other drugs and reagents, unless otherwise indicated, were obtained from Sigma-Aldrich.
Salinas A.G., Lee J.O., Augustin S.M., Zhang S., Patriarchi T., Tian L., Morales M., Mateo Y, & Lovinger D.M. (2023). Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fluorescent sensor. Nature Communications, 14, 5915.
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6

Neurotransmitter Receptor Pharmacology

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Dopamine-HCl and (±)-sulpiride were obtained from Sigma-Aldrich (St. Louis, MO). Quinpirole and DHβE were obtained from Tocris Bioscience (Minneapolis, MN). All drugs were dissolved in voltammetry ACSF.
Salinas A.G., Mateo Y., Carlson V.C., Stinnett G.S., Luo G., Seasholtz A.F., Grant K.A, & Lovinger D.M. (2021). Long-term alcohol consumption alters dorsal striatal dopamine release and regulation by D2 dopamine receptors in rhesus macaques. Neuropsychopharmacology, 46(8), 1432-1441.
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7

Immunomodulatory Effects of Dermal Fibroblasts

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Primary human adult dermal fibroblasts (HDFa, ATCC® PCS-201-012™), mouse macrophages (RAW 264.7), penicillin streptomycin, fetal bovine serum (FBS), phosphate buffered saline (PBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) proliferation assay kit and Dulbecco’s modified eagle medium (DMEM high glucose 4500 mg/L) were all purchased from ATCC (Manassas, VA, USA). DMEM (no glucose), sodium pyruvate and Hoescht dye were obtained from Fischersci (Hanover Park, IL, USA). Lipopolysaccharide pseudomonas aeroguinosa (LPS), Calcein-AM, protease inhibitor cocktail, mouse nitric oxide synthase and dopamine-HCl were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethidium bromide was acquired from Invitrogen (Grand Island, NY, USA). Human recombinant Interferon γ (hrIFN-γ) was purchased from GenScript (Piscataway, NJ, USA). The primary and secondary antibodies against CD90/Thy1 (ab23894) protein and (iNOS) (ab136918) were purchased from Abcam (Cambridge, MA, USA). The 7.5% SDS polyacrylamide gels and gelatin were acquired from Bio-Rad (Hercules, CA, USA). The Odyssey western blot starter kit 2 was obtained from LI-COR (Lincoln, NE, USA). Silicone elastomer base and curing agent (Dow Corning Sylgard®184) were obtained from ML Solar LLC (Campbell, CA, USA).
P. Kwesiga M., Cook E., Hannon J., Wayward S., Gwaltney C., Rao S, & C. Frost M. (2018). Investigative Study on Nitric Oxide Production in Human Dermal Fibroblast Cells under Normal and High Glucose Conditions. Medical Sciences, 6(4), 99.
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8

Enzyme-Catalyzed Silica Nanoparticle Synthesis

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Poly (ethylene glycol)-b-poly (propylene glycol)-b-poly (ethylene glycol) (P123), tetraethoxysilane (TEOS) and dopamine·HCl were purchased from Sigma-Aldrich. 1,3,5-trimethylbenzene (TMB) was purchased from Meryer, China. Lipase Candida sp. 99-125 (the protein concentration in the crude lipase is 104.7 mg/g) was purchased from Beijing CTA New Century Biotechnology Co., Ltd., China. All other reagents were of AR grade.
Gao J., Jiang Y., Lu J., Han Z., Deng J, & Chen Y. (2017). Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125. Scientific Reports, 7, 40395.
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9

Titanium Biocompatibility and Cell Viability

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Commercially pure titanium was purchased from Baoji Nonferrous Metal Co., Ltd. Dopamine-HCl, silver nitrate, rhodamine 123 and MTT were purchased from Sigma-Aldrich. Nitric acid, hydrochloric acid, dimethyl sulfoxide and glutaraldehyde were purchased from Acros Organics. Fetal bovine serum, α-minimum Eagle’s medium, and trypsin-EDTA solution were purchased from Gibco. Other reagents were purchased from Sinopharm Chemical Reagent Co., Ltd.
Wei Z., Li K., Wang S., Wen L., Xu L., Wang Y., Chen Z., Li W., Qiu H., Li X, & Chen J. (2022). Controllable AgNPs encapsulation to construct biocompatible and antibacterial titanium implant. Frontiers in Bioengineering and Biotechnology, 10, 1056419.
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10

Neurotransmitter Modulation Protocols

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All drugs were dissolved in either purified water or dimethyl sulfoxide (DMSO). Stock solutions containing DMSO were made such that the final concentration in slice superfusate was diluted by at least 1000-fold. A769662, dorsomorphin dihydrochloride (compound C), STO609 acetate, and ZD7288 were purchased from Cayman Chemical (USA). Dopamine HCl, GBR12935, cocaine HCl, (−) quinpirole HCl, and (−)-sulpiride were purchased from Sigma–Aldrich (USA). SCH39166 was obtained from Tocris/Bio-techne (UK), and EPPTB (N-(3-ethoxyphenyl)-4-pyrrolidin-1-yl-3-trifluoromethyl-benzamide) was a generous gift from Dr. Aaron Janowsky at VA Portland Health Care System, Portland, OR. A769662 was dissolved to working strength in internal pipette solutions; this agent diffused passively from pipette solutions into the cytosol during whole cell recordings. All other drugs were dissolved to final concentration in aCSF and added to the brain slice superfusate. Typically 60 sec were required for solution changes to traverse the tubing system to show initial effect at the neuron. Dopamine solutions were made daily and kept on ice to retard oxidation.
Yang W., Munhall A.C, & Johnson S.W. (2019). Dopamine evokes a trace amine receptor-dependent inward current that is regulated by AMP kinase in substantia nigra dopamine neurons. Neuroscience, 427, 77-91.
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