DLS intensities were measured for ΔHSPA9 (50 mM CAPS, 200 mM NaCl, 1 mM KCl, 5% (v/v) glycerol, 4 mM β-mercaptoethanol, pH 10), ΔHSPA8 (Buffer 6), and HSPA8-NBD (Buffer 5) using a Zetasizer Nano Z instrument (Malvern Instruments, UK). Data acquisition and manipulation was performed using Zetasizer Nano software. All data were collected using automated settings at 25 °C (ΔHSPA8 and HSPA8-NBD) or 23 °C (ΔHSPA9) a Zen2112 cuvette, a 173° scattering angle, and reported values are the mean of 3 replicates.
Zetasizer nano z instrument
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer Nano Z is a laser-based particle size analyzer from Malvern Panalytical. It measures the size, size distribution, and zeta potential of particles and molecules in liquid samples using the principles of dynamic light scattering and electrophoretic light scattering.
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Lab products found in correlation
Asap 2020 analyzer, by Micrometrics (1 mentions)
Nanosem 450, by Thermo Fisher Scientific (1 mentions)
Vertex 70, by Bruker (1 mentions)
Uv 2600, by Shimadzu (1 mentions)
Fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
Multiskan go microplate reader, by Thermo Fisher Scientific (1 mentions)
5 protocols using zetasizer nano z instrument
1
Dynamic Light Scattering of HSPA Proteins
2
Characterizing Vesicular Formulations: Particle Size, Polydispersity, and Zeta Potential
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The drug release, physical stability, and cellular absorption of vesicles are all affected by particle size and shape. Light scattering is essential for determining the properties of colloidal and macromolecular dispersions. Wet laser diffraction sizing is used primarily to evaluate the characteristics of TEs and is called dynamic light scattering (DLS) [10 (link)].
This approach yields a volume-based primary particle-size distribution. Polydispersity can be measured in terms of uniformity or the degree to which the distribution is symmetric around the median point and the width of the distribution. Small PDI values of 0.1 suggest a homogeneous dispersion, but large values >0.3 imply substantial heterogeneity [39 (link)]. The charge on the vesicular dispersion can influence the formulation’s stability and vesicle interaction with the delivery site. It affects long-term physical stability [40 (link)]. Laser Doppler anemometry with a Zetasizer Nano-Z instrument (Malvern Instruments, UK) was used to evaluate zeta potential [41 (link)].
This approach yields a volume-based primary particle-size distribution. Polydispersity can be measured in terms of uniformity or the degree to which the distribution is symmetric around the median point and the width of the distribution. Small PDI values of 0.1 suggest a homogeneous dispersion, but large values >0.3 imply substantial heterogeneity [39 (link)]. The charge on the vesicular dispersion can influence the formulation’s stability and vesicle interaction with the delivery site. It affects long-term physical stability [40 (link)]. Laser Doppler anemometry with a Zetasizer Nano-Z instrument (Malvern Instruments, UK) was used to evaluate zeta potential [41 (link)].
3
Zeta Potential and Surface Hydrophobicity Analysis
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A Zetasizer Nano Z instrument (Malvern Panalytical, Malvern, UK), equipped with Malvern’s Zetasizer software (version 7.13), was used to determine the zeta potential of the protein samples as described previously Bu [4 (link)]. A fluorescence-based method previously reported by Boyle [22 (link)], without modification, was used to determine the surface hydrophobicity of the protein samples.
4
Characterization of Polymer Particulate Suspensions
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The kinematic viscosities of the suspension electrolytes were measured by a Ubbelohde viscometer at 25 °C. The size distribution of polymer particulates was determined by a dynamic laser scattering (DLS, Bruker BI-200SM) with a red laser source (640 nm) at 25 °C. The Zeta potentials of 0.001 mol L−1 polymer particulate suspensions in 0.002 mol L−1 H2SO4 were collected by a Malvern Zetasizer Nano Z instrument. Fourier transform infrared spectroscopy (FTIR, Bruker Vertex 70) analysis was conducted by using powdered samples (vacuum dried at 50 °C for 12 h before testing). UV-Vis absorption spectra in solid state were collected in the wavelength range of 200–800 nm using BaSO4 as the matrix powder (Shimadzu UV-2600). The optical band gap of PI1 polymer was calculated according to the Tauc plot. UV-Vis absorption spectra of the diluted polymer particulate suspensions at fully-charged and fully-discharged states were measured in the wavelength range of 200–800 nm. Field-emission scanning electronic microscopy (FESEM, FEI NanoSEM-450) was performed to analyze the morphology of polymer particulates by dispersing on a silicon wafer. Nitrogen adsorption/desorption isotherms were recorded at 77 K using a Micrometrics ASAP 2020 analyzer. The surface area and pore size distribution were determined by Brunauer–Emmett–Teller (BET) and Barrett–Joyner–Halenda method, respectively.
5
Characterization of HTCC Nanoparticles
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The particle size and size distribution of HTCC were measured by dynamic light scattering DLS (Varian, Palo Alto, USA). Zeta potential measurements were performed at 25°C on a Malvern Zeta sizer-Nano Z instrument. The nanostructure and size of HTCC were observed by transmission electron microscopy (TEM, Bruker, Germany). UV-vis-NIR absorbance spectra of HTCC, Ce6, HCPT, and HA-tk-CA were observed by Multiskan Go microplate reader (Thermo Fisher Scientific, Massachusetts, USA). The fluorescent signals of HTCC and free Ce6 were measured using a fluorescence spectrophotometer (Varian, Palo Alto, USA). The loading contents for HCPT and Ce6 were determined using a UV-vis-NIR spectrometer at 395 and 665 nm, respectively.
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