The immunoblot analysis was conducted with the following primary antibodies: monoclonal rabbit anti-HK2 antibody (#2024, Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-LDH-A antibody (#3582, Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-PFKP antibody (#8164, Cell Signaling Technology, Danvers, MA, USA), monoclonal rabbit anti-PKM2 antibody (#4053, Cell Signaling Technology, Danvers, MA, USA), and monoclonal mouse anti-β-actin (A5316, Sigma-Aldrich, St. Louis, MO, USA). For nuclear staining and mounting in immunofluorescence analysis, tissue slides were processed with Fluoroshield™ with DAPI (F6057, Sigma-Aldrich, St. Louis, MO, USA). For mounting in the immunohistochemistry analysis, tissues were mounted onto gelatin-coated slides and were processed with Canada Balsam (Wako, Tokyo, Japan) following the dehydration of sections.
Monoclonal rabbit anti hk2 antibody
Manufactured by Cell Signaling Technology
Sourced in United States, United Kingdom
Monoclonal rabbit anti-HK2 antibody is a laboratory reagent that can be used to detect the HK2 protein in biological samples. HK2 is an enzyme involved in glucose metabolism. This antibody can be used in various immunoassay techniques, such as Western blotting, to study the expression and distribution of HK2 in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Monoclonal rabbit anti ldh a antibody, by Cell Signaling Technology (2 mentions)
Canada balsam, by Fujifilm (2 mentions)
Fluoroshield with dapi, by Merck Group (2 mentions)
Ab129068, by Abcam (1 mentions)
Mouse monoclonal anti β actin, by Merck Group (1 mentions)
Monoclonal mouse anti gfap, by Cell Signaling Technology (1 mentions)
2 protocols using monoclonal rabbit anti hk2 antibody
1
Immunoblot Analysis of Metabolic Enzymes
2
Antibody Characterization for Protein Analysis
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The following antibodies were used: polyclonal rabbit anti-NOX2 (ab129068, Abcam, Cambridge, UK), monoclonal mouse anti-NOX2 (NBP1-41012, Novus), monoclonal rabbit anti-HK2 antibody (#2024), monoclonal rabbit anti-LDH-A antibody (#3582, Cell Signaling Technology), monoclonal rabbit anti-LDH-A antibody (#3582, Cell Signaling Technology), polyclonal rabbit anti-COL5A1 antibody (#37304, Cell Signaling Technology), monoclonal mouse anti-GFAP (#3670, Cell Signaling Technology), monoclonal rabbit anti-GFAP (#12389, Cell Signaling Technology), and monoclonal mouse anti-β-actin (A5316, Sigma-Aldrich). Fluoroshield™ with DAPI (F6057, Sigma-Aldrich) was used for nuclear staining and mounting. Sections were mounted onto gelatin-coated slides with Canada Balsam (Wako, Tokyo, Japan) following dehydration.
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