Zen 2012 blue addition software

Manufactured by Zeiss

Sourced in Germany

ZEN 2012 (blue edition) software is a microscope imaging and analysis platform developed by Zeiss. It provides a user interface and tools for controlling Zeiss microscope hardware, capturing images, and performing basic image processing and analysis tasks.

Automatically generated - may contain errors

Lab products found in correlation

Dithizone, by Merck Group (2 mentions) Fluorescein diacetate, by Merck Group (2 mentions) Observer z1, by Zeiss (2 mentions) Axiocam mrm camera, by Zeiss (2 mentions) Propidium iodide, by Merck Group (2 mentions)

Spelling variants of this product

ZEN software (2479 citations) Zen Blue software (487 citations) ZEN 2012 software (475 citations) Zen Blue (223 citations) ZEN blue 2012 software (36 citations) Zen 2012 Blue software (14 citations)

2 protocols using zen 2012 blue addition software

Quantifying Islet Cell Viability

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From each condition, 100-μL samples of islets were combined with 377.6 μL of dithizone (Sigma-Aldrich, St. Louis, MO). To this, 1.39 μL of fluorescein diacetate (Sigma-Aldrich) and 21.05 μL of propidium iodide (Sigma-Aldrich) were added for final concentrations of 0.067 and 4.0 μM, respectively. Islets were incubated in the dark for 20 minutes, after which they were allowed to settle and 100 μL of islets were placed on a slide for imaging. To determine the proportion of live versus dead cells, islets were imaged on a Zeiss Observer.Z1 (Zeiss, Oberkochen, Germany) using the 10× objective and an AxioCam MRm camera with ZEN 2012 (blue addition) software (Zeiss).

Smith K.E., Kelly A.C., Min C.G., Weber C.S., McCarthy F.M., Steyn L.V., Badarinarayana V., Stanton J.B., Kitzmann J.P., Strop P., Gruessner A.C., Lynch R.M., Limesand S.W, & Papas K.K. (2018). Acute Ischemia Induced by High-Density Culture Increases Cytokine Expression and Diminishes the Function and Viability of Highly Purified Human Islets of Langerhans. Transplantation, 101(11), 2705-2712.

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Islet Viability Assay with Dithizone

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From each condition, 100μl samples of islets were combined with 377.6μl of dithizone (Sigma-Aldrich, St. Louis, MO). To this, 1.39μl of fluorescein diacetate (Sigma-Aldrich) and 21.05μl of propidium iodide (Sigma-Aldrich) were added for final concentrations of 0.067μM and 4.0μM respectively. Islets were incubated in the dark for 20 minutes, after which they were allowed to settle and 100μl of islets were placed on a slide for imaging. To determine the proportion of live vs. dead cells, islets were imaged on a Zeiss Observer.Z1 (Zeiss, Oberkochen, Germany) using the 10X objective and an AxioCam MRm camera with ZEN 2012 (blue addition) software (Zeiss).

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