Golden gate

Analyses were carried out using a Brucker Vertex 70 Fourier Transform Infrared Spectroscopy (FTIR) spectrometer equipped with an attenuated total refection (ATR) accessory (Golden Gate, Specac Ltd., Orpington, UK), which is temperature controlled (a heated single-reflection diamond ATR crystal), was used. Films were directly placed in the beam, and eight scans were carried out in the spectrum range of 400 to 4000 cm⁻¹.

Fourier transform
infrared (FTIR) analysis was carried out using an FTIR spectrometer
(model Spectrum One, Perkin Elmer Inc.) equipped with a liquid nitrogen-cooled
mercury–cadmium–telluride (MCT) detector. The spectra
were averaged over 100 scans at a resolution of 4 cm^–1 across the 450–4000 cm^–1 wavenumber range.
Attenuated total reflection (ATR) spectra of 4-bromostyrene, 9-vinylcarbazole,
toluene, and trimethoxyoctylsilane-TiO₂ nanoparticles were
obtained using a single-reflection diamond brazed into a tungsten
carbide accessory (model Golden Gate, Specac Ltd.). Reflection-absorption
infrared spectroscopy (RAIRS) of ASPD hybrid and nanocomposite layer-coated
silicon wafers was performed using a variable angle reflection-absorption
accessory (Specac Ltd.) fitted with a KRS-5 polarizer (to remove the
s-polarized component) set at an angle of 55° to the substrate
normal (sampling depth of 0.5–20 μm for RAIRS⁴⁹ ).

ATR-FTIR spectra of raw materials and printed geometries were recorded using Bruker Vertex 70 Fourier-Transform Infrared Spectrometer (Serial no. GI003417) equipped with diamond ATR accessory (Specac’s Golden Gate™) over a wavenumber range of 500 cm⁻¹ to 4000 cm⁻¹ with a resolution of 2 cm⁻¹ and 128 scans. Spectra were analysed and illustrated using OPUS software version 7.8.

A Bruker Tensor 37 FT-IR
spectrometer (Bruker, Ettlingen, Germany) coupled with the ATR sampling
accessory Golden Gate (Specac Ltd., Orpington, U.K.) was used to obtain
the ATR-FTIR spectra of approximately 50 mg of particles. All spectra
were recorded between 4000 and 600 cm^–1, with a
resolution of 4 cm^–1, and averaging 10 scans. Measurements
were performed in triplicate. The software OPUS 4.0 (Bruker, Ettlingen,
Germany) was used to analyze the spectral data.

ATR-FTIR spectra of the particles were obtained by using a Bruker Tensor 37 FT-IR Spectrometer (Bruker, Ettlingen, Germany) coupled with the ATR sampling accessory Golden Gate (Specac Ltd., Orpington, UK). Approximately 50 mg of liquid oil and encapsulates were deposited onto the diamond crystal to collect the spectra. All spectra were recorded within the wavenumber range 4000–600 cm⁻¹ by averaging 10 scans at 4 cm⁻¹ resolution. Measurements were performed in triplicate. Analysis of spectral data was carried out using the OPUS 4.0 data collection software program (Bruker, Ettlingen, Germany).

Attenuated total reflection (ATR) FTIR spectra of serum samples were recorded with an iS50 FTIR spectrometer (Thermo Nicolet, Madison, WI, USA) using a single-reflection Golden Gate (Specac, Slough, UK) diamond accessory. Measurements were performed using a KBr beamsplitter and a DTGS detector. Interferograms were averaged over 128 scans. Next, they underwent Happ-Genzel apodization and Fourier transformation using a zero-filling factor of 2 to give spectra in the 400-4000 cm⁻¹ range with a resolution of 4 cm⁻¹. A single FTIR spectrum of serum consisted of 7,469 absorbance points.
Before measurement, the frozen serum samples were thawed at room temperature for 30 min. An aliquot of 10 μL of serum was deposited on ATR crystal and nitrogen-dried over 60 min to obtain a thin film of biological material to analyze. After each measurement, the crystal was cleaned with methanol. Serum samples belonging to various groups of women were measured alternately.