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10 nm colloidal gold beads

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10-nm colloidal gold beads are spherical nanoparticles composed of gold with a diameter of 10 nanometers. They are designed for use in various analytical and research applications.

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Lab products found in correlation

Holey carbon grid, by Quantifoil (2 mentions) Mark 4, by Thermo Fisher Scientific (2 mentions) R2 2 holey carbon grids, by Quantifoil (2 mentions)

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10-nm colloidal gold Colloidal gold

4 protocols using 10 nm colloidal gold beads

1

Cryo-EM sample preparation for Legionella

Cited 1 time
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L. pneumophila Lp02 cells were grown as mentioned previously12 (link), harvested at early stationary phase (OD600 of ~3.0), mixed with 10-nm colloidal gold beads (Sigma-Aldrich, St. Louis, MO) precoated with bovine serum albumin, and applied onto freshly glow-discharged copper R2/2 200 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were then blotted and plunge-frozen in a liquid ethane/propane mixture35 (link) using an FEI Vitrobot Mark IV and stored in liquid nitrogen for subsequent imaging.
Ghosal D., Jeong K.C., Chang Y.W., Gyore J., Teng L., Gardner A., Vogel J.P, & Jensen G.J. (2019). Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS. Nature microbiology, 4(7), 1173-1182.
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2

Plunge-freezing of L. pneumophila for cryo-EM

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L. pneumophila Lp02 cells were grown till early stationary stage (OD600 ~2.8) and harvested. Cells were mixed with 10-nm colloidal gold beads (Sigma-Aldrich, St. Louis, MO) precoated with bovine serum albumin. Four μl of this mixture was applied onto freshly glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Using an FEI Vitrobot Mark IV, grids were then blotted (under 100% humidity conditions) and plunge-frozen in a liquid ethane/propane mixture.
Ghosal D., Kim K.W., Zheng H., Kaplan M., Truchan H.K., Lopez A.E., McIntire I.E., Vogel J.P., Cianciotto N.P, & Jensen G.J. (2019). In vivo structure of the Legionella type II secretion system by electron cryotomography. Nature microbiology, 4(12), 2101-2108.
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3

Plunge-freezing of L. pneumophila for cryo-EM

Check if the same lab product or an alternative is used in the 5 most similar protocols
L. pneumophila Lp02 cells were grown till early stationary stage (OD600 ~2.8) and harvested. Cells were mixed with 10-nm colloidal gold beads (Sigma-Aldrich, St. Louis, MO) precoated with bovine serum albumin. Four μl of this mixture was applied onto freshly glow-discharged copper R2/2 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Using an FEI Vitrobot Mark IV, grids were then blotted (under 100% humidity conditions) and plunge-frozen in a liquid ethane/propane mixture.
Ghosal D., Kim K.W., Zheng H., Kaplan M., Truchan H.K., Lopez A.E., McIntire I.E., Vogel J.P., Cianciotto N.P, & Jensen G.J. (2019). In vivo structure of the Legionella type II secretion system by electron cryotomography. Nature microbiology, 4(12), 2101-2108.
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4

Cryo-EM sample preparation for Legionella

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
L. pneumophila Lp02 cells were grown as mentioned previously12 (link), harvested at early stationary phase (OD600 of ~3.0), mixed with 10-nm colloidal gold beads (Sigma-Aldrich, St. Louis, MO) precoated with bovine serum albumin, and applied onto freshly glow-discharged copper R2/2 200 Quantifoil holey carbon grids (Quantifoil Micro Tools GmbH, Jena, Germany). Grids were then blotted and plunge-frozen in a liquid ethane/propane mixture35 (link) using an FEI Vitrobot Mark IV and stored in liquid nitrogen for subsequent imaging.
Ghosal D., Jeong K.C., Chang Y.W., Gyore J., Teng L., Gardner A., Vogel J.P, & Jensen G.J. (2019). Molecular architecture, polar targeting and biogenesis of the Legionella Dot/Icm T4SS. Nature microbiology, 4(7), 1173-1182.
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