The mouse-derived cell line GL261-luc (purchased from PerkinElmer, Waltham, MA, USA) and human-derived cell line LN229 (purchased from ATCC, Manassas, VA, USA) were used in the present study. GL261-luc and LN229 cell lines were cultured in Dulbecco’s modified Eagle’s Medium (DMEM, HyClone, Darmstadt, Germany) with 10% fetal bovine serum (FBS, HyClone) and 1% penicillin-streptomycin (PS, HyClone), and then incubated at a controlled temperature of 37℃ in a 5% CO2 humidified atmosphere.
Gl261 luc
Manufactured by PerkinElmer
Sourced in United States
The GL261-luc is a laboratory equipment product produced by PerkinElmer. It is a cell line that expresses the luciferase reporter gene, which can be used for in vivo bioluminescence imaging studies.
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Lab products found in correlation
3 protocols using gl261 luc
1
Cell Culture Protocol for GL261-luc and LN229 Lines
2
Visualizing Murine Glioblastoma Cells
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Murine-derived glioblastoma cell line stably expressing the luciferase reporter protein (GL261-Luc) was purchased from PerkinElmer (Waltham, MA, USA, catalog number BW134246) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Scientific, Waltham, MA, USA) supplemented with 10% FBS and 1% PenStrep (Wisent Inc, Saint-Jean Baptiste, QC, Canada). For microscopy studies, 50 k GL261 cells were seeded in a coverslip-containing 24-well plate 12 h prior to the experiment for adherence. The following day, HA-LNPs 3× or 5× were added to the cells at the desired final concentration. After overnight incubation, cells were fixed with paraformaldehyde 4%, washed thrice with PBS, and stained with Hoechst 33,342 at 2 μg mL−1 for 30 min, followed by PBS washing and mounting over glass slides for imaging with a Leica TCS SP8 microscope (Wetzlar, Germany). Pictures were analyzed on Fiji software (version 2.9.0/1.53t).
3
Glioblastoma Model and Mesenchymal Stem Cell Therapy
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Mouse glioblastoma GL-261-Luc (PerkinElmer LAS GmbH, Rodgau-Juegesheim, Germany) cells were stereotactically implanted (1 × 105 cells in 2µL of sterile PBS) into the brain (coordinates relative to bregma: DV -3.0; AP +1.0; ML -1.5) of 8-weeks old male C57BL/6 mice (Charles River Laboratories). At day 15 after tumour cells implantation 1 × 105 eGFP+ murine oMSC vs. eGFP+ sMSC (n=7 per group) were injected contralaterally to the position described above. All injections were performed with a 26 gauge needle (Hamilton, Bonaduz, Switzerland). Total injection volume was 2 µL at a flow rate of 1 µL/min. A mixture of 0.5 mg/kg medetomidin, 5.0 mg/kg midazolam and 0.05 mg/kg fentanyl was used for anaesthesia, which was abrogated with 0.5 mg/kg flumazenil and 2.5 mg/kg atipamezole. Animals were euthanized under CO2 at day 7 after injection of murine oMSC/sMSC.
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