ChIP assays were performed as described previously (41 (link)). Sheared chromatin from O3-73 and its derivatives were prepared after fixation with 1% formaldehyde and sonication with Diagenode Bioruptor Pico for 20 cycles (30 s each, vortexed every 5 cycles). ChIP antibodies of rabbit IgG isotypes were coupled to Protein A Dynabeads and were obtained from the following sources: RUNX1 C terminus was provided by Dr. Takeshi Egawa (42 (link)), ETS-1 C20 (Santa Cruz sc-350×), normal rabbit IgG (Santa Cruz sc-2027×), Gal4DBD (Santa Cruz sc-577×), total Histone H3 (Abcam ab1791), H3K4Me3 (Abcam ab8580) and H3K27Ac (Abcam ab4729). ChIP antibodies of mouse IgG isotypes were coupled to Protein G Dynabeads and were obtained from the following sources: p300 (Santa Cruz sc-584×) and normal mouse IgG (Santa Cruze sc-2025). Eluted ChIP and input DNAs were purified with Qiagen QIAquick PCR purification kit and analyzed by qPCR using primers and conditions provided in Table S1 .
Ets 1 c20
Manufactured by Santa Cruz Biotechnology
The ETS-1 C20 is a laboratory instrument designed for the detection and analysis of the ETS-1 protein. It utilizes immunohistochemical techniques to identify the presence and localization of the ETS-1 transcription factor in cellular and tissue samples. The core function of the ETS-1 C20 is to provide researchers with a reliable and standardized method for the assessment of ETS-1 expression levels.
Automatically generated - may contain errors
Lab products found in correlation
Bioruptor pico, by Diagenode (1 mentions)
Normal mouse igg, by Santa Cruz Biotechnology (1 mentions)
Normal rabbit igg, by Santa Cruz Biotechnology (1 mentions)
Qiaquick pcr purification kit, by Qiagen (1 mentions)
Gal4dbd, by Santa Cruz Biotechnology (1 mentions)
H3k4me3, by Abcam (1 mentions)
Total histone h3, by Abcam (1 mentions)
H3k27ac, by Abcam (1 mentions)
Anti ha high affinity clone 3f10, by Roche (1 mentions)
Phosphor akt, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Phosphor erk, by Cell Signaling Technology (1 mentions)
2 protocols using ets 1 c20
1
Chromatin Immunoprecipitation Analysis Protocol
2
Protein Extraction and Immunoblotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tissues were collected from the mice. Protein extracts were prepared by homogenizing samples in tissue lysis PBSTDS buffer [10 mmol/L sodium phosphate (pH 7.3), 154 mmol/L NaCl, 5% sodium deoxycholate, 1% SDS] using a tissue grinder, followed by centrifugation to remove particulate matter and lipids. Immunoblotting was performed as previously described by Lu et al. [19 (link)]. Antibodies used were anti-HA high affinity (clone 3F10, Roche 11867423001), 14-3-3ζ (C-16, Santa Cruz sc-1019), Erk (Cell Signaling 4695), phosphor-Erk (T202/Y204, Cell Signaling 4370), Mek1 (Cell Signaling 9126), phosphor-Mek1 (S221, Cell Signaling 2338), Ets1 (c-20, Santa Cruz sc-350), Ets2 (c-20, Santa Crux sc-351), Akt (Cell Signaling 9272), phosphor-Akt (S473, Cell Signaling 3787), β-actin (Sigma A5441), and tubulin (Sigma T5168). The antibodies were mainly used at a 1:1000 dilution.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!