Analysis of protein expression and IP experiments was performed by resolving proteins by SDS-PAGE, transfer to PVDF and immunoblotting. Primary antibodies used for immunoblotting were: anti-FLAG (Proteintech, 20543-1-AP, 1:4000), anti-HA (Cell Signaling, 2367, 6E2, 1:1125), anti-NAF1 (Abcam, ab157106, 1:1000), anti-NHP2 (Proteintech, 15128–1-AP, 1:5000), anti-NOP10 (Abcam, ab134902, 1:500), anti-TCAB1 (Novus, NB100–68252, 1:2000), anti-reptin (Abcam, ab51500, 1:5000), anti-hTERT (Santa Cruz, sc7215, C-20, 1:500), anti-dyskerin (Santa Cruz, sc-373956, H-3, 1:1500), anti-PARN (Abcam, ab154214, 1:500), anti-RRP40 (Bethyl, A303–909A-T, 1/1500), anti-TIP60 (Santa Cruz, sc5725, N-17, 1/1000), anti-hGAR1 (from Dr Witold Filipowicz (40 (link)), 1/2000) and anti-actin (Chemicon MAB1501, 1:5000).
Anti dyskerin
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-dyskerin is a laboratory reagent used for the detection and quantification of the dyskerin protein. Dyskerin is a protein involved in the maintenance of telomeres and the processing of ribosomal RNA. Anti-dyskerin is a tool used in research applications to study the expression and localization of dyskerin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti flag, by Proteintech (2 mentions)
Ab157106, by Proteintech (2 mentions)
Anti naf1, by Abcam (2 mentions)
Anti nhp2, by Proteintech (2 mentions)
Anti nop10, by Abcam (2 mentions)
Anti gar1, by Proteintech (2 mentions)
Anti ha, by Cell Signaling Technology (1 mentions)
Nb100 68252, by Abcam (1 mentions)
Anti htert, by Santa Cruz Biotechnology (1 mentions)
Anti tip60, by Santa Cruz Biotechnology (1 mentions)
Anti actin, by Merck Group (1 mentions)
Mab1501, by Merck Group (1 mentions)
Ab166630, by Abcam (1 mentions)
H 300, by Abcam (1 mentions)
Ab134902, by Abcam (1 mentions)
Anti nbs1, by Cell Signaling Technology (1 mentions)
Anti rad50, by GeneTex (1 mentions)
Anti mre11, by GeneTex (1 mentions)
Anti parp1, by BD (1 mentions)
Anti trf2, by Novus Biologicals (1 mentions)
5 protocols using anti dyskerin
1
Protein Expression and IP Analysis
2
Characterization of Box H/ACA RNP Components
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The following antibodies were used: anti-NAF1 (Abcam, ab122323) rabbit polyclonal, anti-SHQ1 (27020–1-AP) (Proteintech) rabbit polyclonal, anti-NHP2 (15128–1-AP) (Proteintech) rabbit polyclonal, anti-dyskerin (Santa Cruz H-300) rabbit polyclonal, anti-NOP10 (Abcam, ab134902) rabbit monoclonal, anti-GAR1 (11711–1-AP) (Proteintech) rabbit polyclonal, anti-GAPDH (Abcam, ab9485) rabbit polyclonal, anti-coilin (Bethyl, A303-759A) rabbit polyclonal, anti-nucleolin (Abcam, ab136649) mouse monoclonal, anti-S6 (ThermoS, 9H8L2) rabbit monoclonal, anti-L5 (Bethyl, A303-933A) rabbit polyclonal, anti-U1A (ThermoS, PA5-27474) rabbit polyclonal, and anti-fibrillarin (Abcam, ab166630) rabbit monoclonal.
3
Characterization of Telomeric Protein Complexes
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Whole cell and nuclear extracts were prepared as described previously63 (link). Western blot analysis was performed as described64 (link) with the following primary antibodies: anti-TRF1 (#5745, 0.5 μg/ml)63 (link), anti-TRF2 (4A794, 2.5 μg/ml, Novus Biologicals, Littleton, CO, USA), anti-POT1 (#978, 1:1,000, provided by Dr. Titia de Lange), anti-TIN2 (1:1,500, provided by Dr. Zhou Songyang), anti-TPP1 (#467, 1:1,000, provided by Zhou Songyang), anti-RAP1 (1:1,000, provided by Zhou Songyang), anti-RIF1 (#1060, 1:1,000, provided by Titia de Lange), anti-dyskerin (H-300, 2 μg/ml, Santa Cruz Biotechnology, Dallas, TX, USA), anti-MRE11 (12D7, 1:500, GeneTex, Irvine, CA, USA), anti-RAD50 (13B3, 1:1,000, GeneTex), anti-NBS1 (#3002, 1:1,000, Cell Signaling Technology, Danvers, MA, USA), anti-BLM (ab476, 1:2,000, Abcam, Cambridge, UK), anti-WRN (ab200, 1:2,000, Abcam), anti-ATM (2C1, 2 μg/ml, Santa Cruz Biotechnology), anti-PARP-1 (C2-10, 1:2,000, Pharmingen, Franklin Lakes, NJ, USA), anti-tankyrase-1 (H-350, 2 μg/ml, Santa Cruz Biotechnology), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; RDI-TRK5G4-6C5, 0.5 μg/ml, Research Diagnostics, Flanders, NJ, USA), anti-α-tubulin (B-5-1-2, 1:1,000, Sigma), anti-lamin A/C (636, 2 μg/ml, Santa Cruz Biotechnology) and anti-hTERT (1531-1/Y182, 1:1,000, Abcam). Images were processed with Photoshop CS5 (Adobe).
4
Western Blot Analysis of Protein Complexes
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Analysis of protein expression and IP experiments was performed by resolving proteins by SDS-PAGE, transfer to PVDF and immunoblotting. Primary antibodies used for immunoblotting were: anti-FLAG (Proteintech, 20543-1-AP, 1:4000) or anti-FLAG (Proteintech, 66008-3-Ig, 1:4000), anti-NAF1 (Abcam, ab157106, 1:1000), anti-NHP2 (Proteintech, 15128-1-AP, 1:5000), anti-NOP10 (Abcam, ab134902, 1:500), anti-dyskerin (Santa Cruz, sc-373956, H-3, 1:1500), anti-GAR1 (Proteintech, 11711-1-AP, 1:1000), anti-His (Santa Cruz, sc-8036, H-3, 1:500), and anti-alpha tubulin (Sigma, T5168, 1:5000).
5
Western Blot Analysis of Nucleolar Proteins
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Protein solutions were diluted in Laemmli loading dye (2% SDS, 8% glycerol, 62.5 mM Tris-HCl pH 6.8, 0.005% bromophenol blue, and 2% β-mercaptoethanol) and separated on a 10% gel by SDS-PAGE (TGX Stain-Free™ FastCast™ Acrylamide Solutions; BIORAD) or 4-15% gel (Mini-PROTEAN® TGX Stain-Free™; BIORAD). Proteins were transferred to nitrocellulose membranes (Amersham Protran 0.2um nc) and probed overnight at 4°C with appropriate antibodies. Detection was performed with appropriate HRP-conjugated secondary antibodies and chemiluminescent detection was performed on a ChemiDoc XRS+ Imaging System (BIORAD) using ImageLab v5.1.1 (BIORAD). The Stain-Free system makes it possible for us to check the quality and quantity of the loaded proteins, especially for RIP and polysome protein gels. The following antibodies were used: anti-Dyskerin (Santa Cruz Biotechnology, sc-373956), anti-NHP2 (Santa Cruz Biotechnology, sc-398430), anti-NOP10 (Cusabio, CSB-PA873610LD01HU), anti-GAR1 (Proteintech, 11711-1-AP), anti-RPS14 (Santa Cruz Biotechnology, sc-68873), anti-RPL5 (Bethyl, A303-933A), anti-EIF4GI (Cell Signaling, 2858S), anti-Vimentin (Cell Signaling, 5741S), anti-GAPDH (Sigma-Aldrich, G8795), anti-b-Actin (Sigma-Aldrich, A2228), Lamin B (Santa Cruz Biotechnology, sc-6216).
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