B6 cg tg itgax venus 1mnz j

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B6.Cg-Tg(Itgax-Venus)1Mnz/J is a transgenic mouse strain that expresses the Venus fluorescent protein under the control of the Itgax (CD11c) promoter. This allows for the identification and isolation of CD11c-expressing cells, such as dendritic cells, using fluorescence-based techniques.

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4 protocols using b6 cg tg itgax venus 1mnz j

Multimodal Immune Cell Profiling

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C57BL/6J, B6.129(Cg)-Ccr2^tm2.1Ifc/J (CCR2-RFP), B6.Cg-Tg(Itgax-Venus)1Mnz/J (CD11c-YFP), B6(Cg)-Tyr^c−2J/J (B6 albino), B6.Cg-Zbtb46^{tm4.1(HBEGF)MnzTyrc−2J}/J (Zbtb46-DTR), B6.129P2(C)-Ccr7^tm1Rfor/J (CCR7.KO), B6.129S1-Il12^btm1Jm/J (IL-12p40.KO), and B6.129S4-Ccr2^tm1Ifc/J (CCR2.KO) mouse strains were obtained from The Jackson Laboratory. CD45.1⁺ B6.Cg-Tg(TcraTcrb)425Cbn/J (OT-II), CD45.1⁺ C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-I), and B6.SJL-Ptprc^aPepc^b/BoyCrl (CD45.1⁺) were obtained either from donating investigators (Dr. Pamela J. Fink, University of Washington) or Charles River. CD11c-YFP animals were crossed with B6 albino mice to homozygosity, and next crossed to CCR2-RFP mice to generate a CD11c-YFP x CCR2-RFP^Hetrozygous dual reporter mice. CCR2-DTR mice were obtained from donating investigators (Dr. Steven F. Ziegler, Benaroya Research Institute) and with approval from the originating investigators (Drs. Tobias M. Hohl and Eric G. Pamer, Memorial Sloan-Kettering Cancer Center) (95 (link)). 6–10 week-old male and female mice were kept in specific pathogen–free conditions at an Association for Assessment and Accreditation of Laboratory Animal Care–accredited animal facility at the University of Washington, South Lake Union campus. All procedures were approved by the University of Washington Institutional Animal Care and Use Committee.

Leal J.M., Huang J.Y., Kohli K., Stoltzfus C., Lyons-Cohen M.R., Olin B.E., Gale M J.r, & Gerner M.Y. (2021). Innate cell microenvironments in lymph nodes shape the generation of T cell responses during Type-I inflammation. Science immunology, 6(56), eabb9435.

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Transgenic Mouse Strains for Immunology

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Sex-matched 6- to 10-week-old BALB/c (H-2d; National Cancer Institute/NIH or The Jackson Laboratory), C57BL/6 (B6; H-2b; National Cancer Institute/NIH or The Jackson Laboratory), B6.SJL-Ptprc^aPepc^b/BoyJ (B6.CD45.1; H-2b; National Cancer Institute/NIH or The Jackson Laboratory), B6.Cg-Tg(Itgax-Venus)1Mnz/J (B6.CD11c-YFP; The Jackson Laboratory), C57BL/6-Foxp3tm1Flv/J (B6.Foxp3-RFP; The Jackson Laboratory), B6.129S1-Nt5e^tm1Lft/J (B6.CD73^–/–; The Jackson Laboratory), B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J (B6.CFP; The Jackson Laboratory), C57BL/6-Tg(CAG-EGFP)1Osb/J (B6.eGFP; The Jackson Laboratory), and B6.PL-Thy1^a/CyJ (B6.CD90.1; The Jackson Laboratory) mice were used. B6.Cg-Ltβr^tm1Mmat/Rbrc (B6.LTβR^–/–) were a gift from Mitsuru Matsumoto (The University of Tokushima, Tokushima, Japan) (50 (link)). B6.Foxp3-RFP mice were crossed with B6.CD90.1 in our facility. B6.CD73^–/– mice were crossed with B6.Foxp3-RFP.CD90.1 in our facility. In addition, B6.Foxp3-RFP.CD90.1 and B6.Foxp3-RFP.CD90.CD73^–/– mice were further crossed with B6.eGFP in our facility. Mice were housed with food and water ad libitum.

Dai H., Pena A., Bauer L., Williams A., Watkins S.C, & Camirand G. (2022). Treg suppression of immunity within inflamed allogeneic grafts. JCI Insight, 7(16), e160579.

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Itgax-Venus Mouse Model Protocols

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Male/female Itgax-Venus mice (B6.Cg-Tg(Itgax-Venus)1Mnz/J) [34 (link)] were purchased from Jackson Laboratory (Bar Harbor, ME). Mice from E12.5 to P56 were used in each experiment. Mice were housed in groups at a temperature of 22 ± 1 °C with a 12-hour light–dark cycle, and were fed food and water ad libitum. All animal experiments were conducted according to relevant national and international guidelines contained in the ‘Act on Welfare and Management of Animals’ (Ministry of Environment of Japan) and ‘Regulation of Laboratory Animals’ (Kyushu University) and under the protocols approved by the Institutional Animal Care and Use committee review panels at Kyushu University.

Nomaki K., Fujikawa R., Masuda T, & Tsuda M. (2024). Spatiotemporal dynamics of the CD11c+ microglial population in the mouse brain and spinal cord from developmental to adult stages. Molecular Brain, 17, 24.

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Generation of Sema4A, Plexin-B1 Knockout Mice

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Sema4A knockout ES clones (ID: EPD0105_3_B05) were purchased from EUCOMM and microinjected into C57BL/6 blastocysts. To generate constitutive Sema4A knockout mice, mice carrying the Sema4A “knockout-first” allele were crossed with E2a-Cre mice. The successful knockout of Sema4A was confirmed by Southern blotting. B6.Cg-Tg(Itgax-Venus)1Mnz/J expressing YFP under the CD11c promoter were purchased from The Jackson Laboratory. To generate mice lacking Sema4A and expressing YFP in CD11c-positive cells, Sema4A knockout mice were crossed with mice expressing YFP under the control of the CD11c (Itgax) promotor. Plexin-B1 knockout mice were generated as described previously (Deng et al., 2007 (link)). Mice were maintained under specific pathogen-free conditions. All procedures involving animals were performed in accordance with German Animal Welfare legislation.

Sun T., Yang L., Kaur H., Pestel J., Looso M., Nolte H., Krasel C., Heil D., Krishnan R.K., Santoni M.J., Borg J.P., Bünemann M., Offermanns S., Swiercz J.M, & Worzfeld T. (2017). A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib. The Journal of Cell Biology, 216(1), 199-215.

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