Nih3t3 fibroblasts

Cytocompatibility of SF-WS and SF-EGCG was evaluated using the MTT method. NIH3T3 fibroblasts (Korea Cell Line Bank, Korea) were maintained in Dulbecco’s Modified Eagles medium (DMEM) supplemented with 10% (v/v) fetal bovine serum. The cells were seeded in a 96-well plate at a density of 10⁴ cells/well and then cultured for 24 h (37°C, 5% CO₂). After the medium was removed, the cells were treated with various concentrations of SF-WS and SF-EGCG for 24 h at 37°C. After rinsing with PBS solution, the cells were treated with 100 µL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent for 4 h at 37°C. After MTT reagent was removed out, 100 µL of dimethyl sulfoxide was added to each well. After 30 min, the absorbance at 540 nm was measured using a microplate reader (Autobio, China).

NIH3T3 fibroblasts purchased from Korean Cell Line Bank (KCLB) were cultured in high-glucose Dulbecco’s Modified Eagle’s Medium (DMEM; Welgene, Daegu, Korea) supplemented with 10% FBS and 1% penicillin-streptomycin in a 5% CO₂ atmosphere at 37°C, and passaged twice per week.

Cytocompatibility of SF-WS and SF-EGCG was evaluated using the MTT method. NIH3T3 fibroblasts (Korea Cell Line Bank, Korea) were maintained in Dulbecco’s Modified Eagles medium (DMEM) supplemented with 10% (v/v) fetal bovine serum. The cells were seeded in a 96-well plate at a density of 10⁴ cells/well and then cultured for 24 h (37°C, 5% CO₂). After the medium was removed, the cells were treated with various concentrations of SF-WS and SF-EGCG for 24 h at 37°C. After rinsing with PBS solution, the cells were treated with 100 µL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent for 4 h at 37°C. After MTT reagent was removed out, 100 µL of dimethyl sulfoxide was added to each well. After 30 min, the absorbance at 540 nm was measured using a microplate reader (Autobio, China).