F1804

Manufactured by Novus Biologicals

The F1804 is a laboratory centrifuge designed for general-purpose applications. It features a compact design, digital speed and time controls, and accommodates a range of rotor sizes. The core function of the F1804 is to separate components of a liquid mixture based on differences in their densities through the application of centrifugal force.

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Lab products found in correlation

Fibrillarin g 8, by Santa Cruz Biotechnology (1 mentions) U2af65, by Merck Group (1 mentions) Igpal ca 630, by Merck Group (1 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions) Pierce protein a g magnetic beads, by Thermo Fisher Scientific (1 mentions) Phospho histone h2a x ser139, by Cell Signaling Technology (1 mentions) Gapdh, by Merck Group (1 mentions) Edta free protease inhibitor cocktail, by Roche (1 mentions) Rpa194 c 1, by Santa Cruz Biotechnology (1 mentions) Abs16, by Merck Group (1 mentions) Hvin 1, by Merck Group (1 mentions)

2 protocols using f1804

Comprehensive Antibody Immunodetection Protocol

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Antibodies recognizing the Flag tag (Sigma,F1804), SETX (Novus, NBP1-94712), RNA polymerase I (RPA194 (C-1) (Santa Cruz, sc-48385), fibrillarin (G-8) (Santa Cruz, sc-374022), nucleophosmin (NPM1, Forties Life Sciences A302-403A-T), C1QBP/P32 (Proteintech, 24474-1-AP), CHCHD2 (Proteintech, 66302-1-lg), GAPDH (Sigma-Aldrich, G9545), U2AF65 (Sigma-Aldrich, U4758), H3 (Abcam, ab8898), phospho-histone H2A.X(Ser139) (Cell signaling, 2577), and MitoTracker Red CMXRos (Invitrogen, M7512) were obtained from the indicated vendors. Human RIEP rabbit polyclonal antibodies were generated at Bethyl Laboratories/Fortis Life Sciences. RIEP-1 and RIEP-216 antibodies recognize peptides derived from the RIEP N terminus or C terminus, respectively. IGPAL-CA-630 (Sigma, I8896), cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail (Roche, 04693159001), and Pierce™ Protein A/G Magnetic Beads (Thermo Fisher scientific, 88802) were purchased from indicated vendors.

Feng S., Desotell A., Ross A., Jovanovic M, & Manley J.L. (2023). A nucleolar long “non-coding” RNA encodes a novel protein that functions in response to stress. Proceedings of the National Academy of Sciences of the United States of America, 120(9), e2221109120.

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Antibody List for Immunoblotting

Cited 1 time

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The antibodies used in this study were: GAPDH polyclonal antibody (EMD Millipore #ABS16, 1/20000), Flag polyclonal antibody (Sigma-Aldrich, M2, #F7425, 1/3000), Flag monoclonal antibody (Sigma-Aldrich #F1804, 1/1000), UNG polyclonal antibody (Novus biologicals # NBP1-49985, 1/1500), RPA32 monoclonal antibody (Invitrogen, 9H8, #MA1-26418,1/2000), Vinculin monoclonal antibody (Sigma-Aldrich, hVin-1, #V9264, 1/5000), and APOBEC3B monoclonal antibody (5210-87-13; NIH-ARP #12398, 1/1000)^{92 (link)}.

Sanchez A., Ortega P., Sakhtemani R., Manjunath L., Oh S., Bournique E., Becker A., Kim K., Durfee C., Temiz N.A., Chen X.S., Harris R.S., Lawrence M.S, & Buisson R. (2024). Mesoscale DNA features impact APOBEC3A and APOBEC3B deaminase activity and shape tumor mutational landscapes. Nature Communications, 15, 2370.

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