Buv 395cd4

Manufactured by BD

The BUV-395CD4 is a fluorochrome-conjugated antibody that binds to the CD4 surface antigen on T cells. It is designed for use in flow cytometry applications to identify and analyze CD4+ T cell populations.

Automatically generated - may contain errors

Lab products found in correlation

Countbright beads, by Thermo Fisher Scientific (1 mentions) Cd8 apc cy7, by BD (1 mentions) Annexin 5 percp cy5, by BioLegend (1 mentions) Alexa fluor 647 conjugated goat anti mouse fab, by Jackson ImmunoResearch (1 mentions) Zombie nir, by BioLegend (1 mentions) Facsaria cell sorter, by BD (1 mentions) Lsr 2, by BD (1 mentions) Liberase, by Merck Group (1 mentions) Cd45 fitc, by BioLegend (1 mentions) Bv421 gr 1, by BioLegend (1 mentions) Cd19 buv395, by BD (1 mentions) Cd11b apc cy7, by BioLegend (1 mentions) Fortessa flow cytometer, by BD (1 mentions) Cd11c pe cy7, by BioLegend (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions) Facsaria 3 sorter, by BD (1 mentions)

2 protocols using buv 395cd4

Comprehensive Cytometric Analysis of CAR T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

All antibodies were titrated. CAR expression was measured with Alexa-Fluor-647–conjugated goat anti-mouse Fab (Jackson ImmunoResearch cat#115-607-003, RRID: AB_2338931). The following antibodies were used: CD19–PE (clone SJ25C1 BD Biosciences cat# 340364, RRID: 400018) or CD19–BUV395 (clone SJ25C1, BD Biosciences cat# 563551, RRID: AB_2738272), CD19–BV510 (clone SJ25C1, Biolegend cat#363020, RRID: AB_2564229), CD3–BUV737 (clone UCHT, BD Biosciences cat# 612750, RRID: RRID:AB_2870081), BUV-395CD4 (BD Biosciences cat# 563552, RRID:AB_2738273), and APC-cy7-CD8 (BD Biosciences cat# 557834, RRID:AB_396892). CountBright beads (Invitrogen cat# C36950) were used to determine the absolute number of cells according to the manufacturer’s protocol. 7-AAD or DAPI was used to exclude dead cells. Fc Receptor–Binding Inhibitor Antibody Human (eBioscience cat# 14-9161-73, RRID: AB_468582) was used to block Fc receptors. In vitro viability after RT was assessed using Zombie NIR (Biolegend cat# 423106) and Annexin V PerCP-Cy5.5 (Biolegend cat# 640936) according to the manufacturer’s protocols. Data were collected using BD LSR-II, BD LSR-Fortessa, and Cytek Northern Lights cytometers. Data were analyzed with FlowJo Software (Treestar/BD Biosciences, RRID: SCR_008520). Cell sorting was performed using a BD FACSAria cell sorter.

Kim A.B., Chou S.Y., Kang S., Kwon E., Inkman M., Szymanski J., Andruska N., Colgan C., Zhang J., Yang J.C., Singh N, & DeSelm C.J. (2023). Intrinsic tumor resistance to CAR T cells is a dynamic transcriptional state that is exploitable with low-dose radiation. Blood Advances, 7(18), 5396-5408.

+ Open protocol

+ Expand

Multiparameter Flow Cytometry Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flow cytometry was performed as previously described [9 (link)]. The following Abs were used: BV785-CD3ε (Clone 145-2C11), BV711-CD8α (Clone 53–6.7), APC/Cy7-CD11b (Clone M1/70), BV-421-Gr-1 (Clone RB6-8C5), PE/Cy7-CD11c (Clone N418), FITC-CD45 (Clone I3/2.3), PerCP/Cy5.5-IA^b (Clone AF6-120.1), BV605-NK1.1 (PK136) were from Biolegend. BUV395-CD4 (Clone GK1.5) and BUV395-CD19 (Clone 1D3) were from BD Biosciences. To obtain single-cell suspensions, LNs were first incubated in Liberase TM (1.67 Wünsch units/mL) (Sigma) in PBS with 25 mM HEPES for 30 min at 37°C before adding PBS with 25 mM HEPES + 10% FBS to halt the digestion process, followed by mechanical disruption of the tissue through a 70-μm filter. Cells were washed once with FACS buffer (PBS with 1% BSA + 0.1% sodium azide) before surface staining. For analysis, samples were acquired using either a BD LSR II flow cytometer or a BD Fortessa flow cytometer (BD Biosciences), and data were analyzed with FlowJo software (TreeStar). For cell sorting, samples were acquired with a BD FACS Aria III sorter (BD Bioscience).

Wong E.B., Montoya B., Ferez M., Stotesbury C, & Sigal L.J. (2019). Resistance to ectromelia virus infection requires cGAS in bone marrow-derived cells which can be bypassed with cGAMP therapy. PLoS Pathogens, 15(12), e1008239.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!