Fragaria vesca leaves were acquired from Granja de Figueira do Lorvão, Penacova, Portugal, on May 2011. Ethanol, acetonitrile, formic acid, sodium carbonate, and Folin Ciocalteu reagent were obtained from Merck (Lisboa, Portugal). Tetrahydrofuran (THF) was obtained from Koch-Light (Johannesburg, Gauteng, South Africa); iron (III) chloride hexahydrate 98% from Acros (Queluz, Portugal). HPLC methanol, came from Merck (Lisboa, Portugal). Hydroxyethyl cellulose (HEC) was obtained from Ashland (Wilmington, DE, USA). l -DOPA (0.5 mM), mushroom tyrosinase, pyrogallol, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified eagle medium (DMEM) with stable glutamine and foetal bovine serum (FBS) were purchased from Bioconcept (Allschwil, Switzerland).
Hplc methanol
Manufactured by Merck Group
Sourced in United States
HPLC-methanol is a chromatographic solvent used as the mobile phase in high-performance liquid chromatography (HPLC) analysis. It is a clear, colorless, and volatile liquid with the chemical formula CH3OH. HPLC-methanol is a commonly used solvent in various analytical applications due to its ability to effectively separate and detect a wide range of compounds.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Merck Group (2 mentions)
Formic acid, by Merck Group (2 mentions)
Hplc water, by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (2 mentions)
Iron 3 chloride, by Merck Group (2 mentions)
Quercetin hydrate, by Merck Group (2 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Folin ciocalteu reagent, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Pyrogallol, by Merck Group (1 mentions)
Iron 3 chloride hexahydrate, by Thermo Fisher Scientific (1 mentions)
Hydroxyethyl cellulose hec, by Ashland (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Triethylamine, by Merck Group (1 mentions)
Methacrylic anhydride, by Merck Group (1 mentions)
Salicylic acid, by Merck Group (1 mentions)
Magnesium sulfate, by Merck Group (1 mentions)
Dmem medium, by Merck Group (1 mentions)
11 protocols using hplc methanol
1
Extraction and Analysis of Bioactive Compounds from Fragaria vesca
2
Butterfly Pea Flower Extraction Protocol
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HPLC methanol, acetonitrile, formic acid, and acetic acid were purchased from Merck KGaA, Darmstadt, Germany. All chemicals were analytical grade. Milli-Q water (Milli-Q IQ 7003/7005, Merck, NJ, USA) was used.
Butterfly pea flowers were grown at the College of Agriculture, Can Tho University, Vietnam. Butterfly pea flowers were freeze-dried in an Alpha 2–4 DL freeze dryer (Martin Christ, Germany) at −80 °C and 0.001 mbar to 3−5% moisture content and finely ground before extraction.
Butterfly pea flowers were grown at the College of Agriculture, Can Tho University, Vietnam. Butterfly pea flowers were freeze-dried in an Alpha 2–4 DL freeze dryer (Martin Christ, Germany) at −80 °C and 0.001 mbar to 3−5% moisture content and finely ground before extraction.
3
Functionalized Silicone Rubber for Cell Culture
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Silicone rubber (SR) (1 mm in thickness) was provided by Goodfellow (HuntingdonUK). We washed the SR with ethyl alcohol for 24 h to remove the impurities and dried it under a vacuum (60 °C) for 3 h. All the synthesis reagents (methacrylic anhydride, salicylic acid, dimethylaminopyridine (DMAP), dichloromethane, triethylamine, magnesium sulfate, ethyl ether, p-dioxane, ethanol, toluene, and petroleum ether) were from Sigma-Aldrich (San Luis, MO, USA), DMEM medium, fetal bovine serum, streptomycin solution, gentamicin, HPLC acetonitrile and HPLC methanol, and distilled water were used as received Sigma-Aldrich (San Luis, MO, USA). The MTT kit was from Roche (Basel, Switzerland)
4
Analytical Characterization of SCRA Samples
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Some samples
were extracted from
(Police seized) street material prepared as we have recently described.18 (link),19 (link) Some SCRAs were purchased from Cayman Chemical Co. (Ann Arbor, MI,
USA). Samples were dissolved in either deionized water, purified using
an Elix essential water purification system (Merck Millipore, Burlington,
MA, USA), or HPLC methanol >99.9% purity (Sigma-Aldrich, St Louis,
MO, USA). Oral fluid samples were collected from volunteers who confirmed
no legal or illegal drug use in the preceding month. The oral fluid
samples were passed through a 0.44 μm syringe-driven filter.
were extracted from
(Police seized) street material prepared as we have recently described.18 (link),19 (link) Some SCRAs were purchased from Cayman Chemical Co. (Ann Arbor, MI,
USA). Samples were dissolved in either deionized water, purified using
an Elix essential water purification system (Merck Millipore, Burlington,
MA, USA), or HPLC methanol >99.9% purity (Sigma-Aldrich, St Louis,
MO, USA). Oral fluid samples were collected from volunteers who confirmed
no legal or illegal drug use in the preceding month. The oral fluid
samples were passed through a 0.44 μm syringe-driven filter.
5
Methanol Solvent Characterization and Antioxidant Evaluation
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Methanol (MeOH) was purchased from El-Nasr Company for Pharmaceuticals and Chemicals (Cairo, Egypt). Solvents for High-Performance Liquid Chromatography (HPLC) were purchased from Sigma-Aldrich (Saint Louis, MO, USA), including HPLC-methanol and HPLC-water. For the biological study, DPPH, rutin, and ascorbic acid were purchased from (Sigma-Aldrich, Saint Louis, MO, USA).
6
Synthesis of IronQ Complex
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The IronQ complex was synthesized following the methodology described in our previous study.26 (link) Shortly, stirring quercetin hydrate (0.0050 moles) (Sigma, USA) in a round bottle with 500 mL HPLC-methanol (Sigma, USA) until complete dissolution, the solution’s color shifted yellow. Then, the PH of mixed solution was gradually modified to 12 using a NaOH solution. Subsequently, Iron (III) chloride (Sigma, USA) solution was freshly prepared by dissolving 0.0025 moles of Iron (III) chloride in 500 mL of ultrapure water (up water) to integrate with the quercetin solution resulting in a dark yellow mixture, which was subsequently incubated for 2 hours at 60°C under a continued stirring. After that, the total solution was filtered and evaporated to dryness. The resulting dark powder was collected and reserved it at room temperature (RT) in a drying apparatus, protected from the light.
7
Synthesis of Magnetically-Responsive Polymer Nanoparticles
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N-Isopropylacrylamide (NIPAAm) was re-crystallized from n-hexane and acrylic acid (AA) was purified by vacuum distillation in order to remove the inhibitors before being used as the monomers. The RAFT chain-transfer agent, S,S′-bis(α,α′-dimethyl-α″-acetic acid)trithiocarbonate (CMP) was synthesized, following the protocol proposed by Lai and his coworkers [19 (link)]. Azobisisobutyronitrile (AIBN) was re-crystallized from methanol and then used as the thermal initiator. Rhodamine 6G (R6G), the model drug was purchased from Sigma-Aldrich. Iron(II) chloride tetrahydrate (FeCl2 · 4H2O) and iron(III) chloride (FeCl3) were used as the precursors of magnetic iron oxide (Fe3O4) nanoparticles. Ammonia solution (33%) was used as the reducing agent. 3-Aminopropyl triethoxysilane (APTES), NHS and EDC were introduced for surface modification. Over 99% of HPLC methanol was purchased from Sigma-Aldrich. Deionized water (18.2 MΩ) was used throughout the work. All chemicals and solvents were purchased from Sigma-Aldrich (Taipei, Taiwan), Acros (Taipei, Taiwan), or Fluka (Taipei, Taiwan) and used as received except otherwise noted.
8
Solvent Selection and Chromatographic Techniques
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The solvents used in this work included n-hexane (n-hex., boiling point b.p. 60–80 °C), dichloromethane (DCM), ethyl acetate (EtOAC), n-butanol (n-but.), ethanol (EtOH), methanol (MeOH), formic acid (F.A.), glacial acetic acid (G.A.A.) and sulfuric acid purchased from El-Nasr Company for Pharmaceuticals and Chemicals (Egypt). High Performance Liquid Chromatography (HPLC) and deuterated solvents used for chromatographic and spectroscopic analyses were purchased from Sigma-Aldrich (Saint Louis, Missouri, USA), including HPLC-methanol, HPLC-water, chloroform (CDCl3), methanol (CD3OD), and dimethyl sulfoxide (DMSO-d6). Column chromatography (CC) was performed using silica gel 60 (63–200 μm, E. Merck, Sigma-Aldrich), and polyamide-6 (50–160 μm), while silica gel GF254 for Thin-layer chromatography (TLC) (El-Nasr Company for Pharmaceuticals and Chemicals, Egypt) was employed for vacuum liquid chromatography (VLC). Thin-layer chromatography (TLC) was carried out using pre-coated silica gel 60 GF254 plates (E. Merck, Darmstadt, Germany; 20 × 20 cm, 0.25 mm in thickness). Spots were visualized by spraying with para-anisaldehyde (PAA) reagent (85 : 5 : 10 : 0.5 absolute EtOH : sulfuric acid : G.A.A. : para-anisaldehyde), followed by heating at 110 °C.17 (link) For the biological study, DPPH and l -ascorbic acid were purchased from (Sigma-Aldrich, Saint Louis, Missouri, USA).
9
Synthesis of the IronQ Complex
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The IronQ complex was synthesized according to our previous research work [25 (link)]. Shortly, 0.0050 mol of quercetin hydrate (Sigma, USA) was supplemented to 500 mL HPLC-methanol (Sigma, USA) with continued stir until complete dissolution of quercetin hydrate, showing the yellow solution. The pH of the solution was then slowly adjusted to 12 based on the addition of NaOH solution to obtain a deprotonated form of quercetin. 0.0025 mol Iron (III) chloride (Sigma, USA) in 500 mL ultrapure water (up water) was then blended with the above solution until the solution color turned to dark yellow, which was followed by incubation with a continuous stirring for 2 h at 60 °C. And then the final solution was purified and evaporated to dryness. The dark powder product was collected and stored it away from light at room temperature (RT).
10
Quantification of α-DCs using HPLC
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Analytical-grade 3-DG was obtained from Toronto Research Chemicals (Toronto, ON, Canada), and GO (40% in water), MGO (32% in water), and 4-nitro-1,2-phenylenediamine (NPDA) were bought from Fluka (Shanghai, China). The 100 mM stock solutions of the three α-DCs were prepared in Millipore water, and NPDA (20.0 mM) was dissolved in methanol. All these solutions were kept at 4 °C until use. HPLC-methanol was from Sigma-Aldrich (Beijing, China). A Milli-Q water purification system (Milli-Q CLX 7000, Merck, Shanghai, China) was used to produce water of high purity. All other chemicals were of analytical purity and were used directly without further purification.
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