Uplans apo objective lens

Manufactured by Olympus

Sourced in United States

The UplanS-Apo objective lens is a high-performance microscope objective lens designed for use in various laboratory applications. It features a superior optical design that provides excellent image quality and resolution. The lens is suitable for a range of magnification levels and can be used with a variety of microscope systems.

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Lab products found in correlation

Coolsnap hq2 camera, by Teledyne (5 mentions) Deltavision mpx microscope, by Cytiva (5 mentions) Glass chambers, by Thermo Fisher Scientific (2 mentions) Deltavision softworx, by Cytiva (2 mentions) Leibovitz s l 15 medium, by Thermo Fisher Scientific (2 mentions) Lsm 880, by Zeiss (2 mentions) Mineral oil, by Merck Group (1 mentions) Sir dna, by Spirochrome (1 mentions) Ix 71 inverted microscope, by Cytiva (1 mentions) Planapo, by Zeiss (1 mentions) Efficient navigation zen , by Zeiss (1 mentions) Axio imager m2, by Zeiss (1 mentions) Axiocam 506, by Zeiss (1 mentions) Lsm 800, by Zeiss (1 mentions) Fm4 64, by Thermo Fisher Scientific (1 mentions)

8 protocols using uplans apo objective lens

Histological Evaluation of Aortic Elastin

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Abdominal aortic tissues were obtained from patients undergoing AAA repair surgery and from brain-dead but heart beating organ donors. The characteristics of the screening and validation cohort are shown in Table 1. The excised segments of the abdominal aorta were collected in RNAlater^® stabilization solution (Ambion Inc., MN, USA) and stored at -20 °C until assayed.
To check if the tissues from organ donors were adequate to use as a control, we applied Verhoeff´s histological staining to assess elastin, an extracellular matrix protein. The image was acquired by a Scanscope CS system unit (Aperio Technologies Inc., CA, USA), with an Olympus UPlanSApo objective lens, with specifications of 20x magnification power and 0.75 numerical aperture attached to the scanner. Images were analyzed using the Aperio program ImageScope View software (Aperio Technologies, Inc., CA, USA). Results were reported as the positive staining percentage area per total tissue area.

de Araujo N.N., Lin-Wang H.T., Germano J.D., Farsky P.S., Feldman A., Rossi F.H., Izukawa N.M., Higuchi M.D., Savioli Neto F., Hirata M.H, & Bertolami M.C. (2019). Dysregulation of microRNAs and target genes networks in human abdominal aortic aneurysm tissues. PLoS ONE, 14(9), e0222782.

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3D Cell Culture Imaging and Tracking Protocol

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Cells were grown in glass chambers (Thermo Fisher Scientific). At 1 h before imaging, the medium was changed to pre‐warmed Leibovitz's L‐15 medium (Thermo Fisher Scientific) supplemented with 20% FBS and 20 mM HEPES, pH 7.0. In 3D culture conditions, spheroid cultured cells were dropped into a 3.5‐cm glass‐bottomed dish (MatTek Corporation) with REBM supplemented with B‐27 Supplement, N‐2 supplement, and 10 μM ROCK inhibitor Y‐27632, and covered with mineral oil (Merck). The dish was coated with 0.5% poly(2‐hydroxyethyl methacrylate) (Merck) in 95% ethanol before cell seeding for 24 h. Cells were treated with SiR‐DNA (Spirochrome) for 6 h before imaging. Recordings were made at 37°C, as described previously.²⁵, ²⁶, ²⁷, ²⁸, ²⁹ Z‐series of three sections in 3‐μm increments were captured every 3 min. Image stacks were projected. Images were collected with an Olympus IX‐71 inverted microscope controlled by DeltaVision softWoRx (Cytiva) using a ×20 0.75 NA UPlanSApo objective lens (Olympus). All cells were tracked using the ImageJ program with the plug‐in TrackMate.³⁰

Iemura K., Anzawa H., Funayama R., Iwakami R., Nakayama K., Kinoshita K, & Tanaka K. (2022). High levels of chromosomal instability facilitate the tumor growth and sphere formation. Cancer Science, 113(8), 2727-2737.

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Real-Time Cell Imaging Protocol

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Cells were grown in glass chambers (Thermo Fisher Scientific). One hour before imaging, the medium was changed to prewarmed Leibovitz’s L‐15 medium (Thermo Fisher Scientific) supplemented with 20% FBS and 20 mM HEPES, pH 7.0. Recordings were made in a temperature‐controlled incubator at 37°C, as described previously.¹¹ (link), ¹⁷ (link), ¹⁸ (link), ¹⁹ (link), ²⁰ (link) Z‐series of three sections in 3‐µm increments were captured every 15 minutes. Image stacks were projected. Images were collected with an Olympus IX‐71 inverted microscope controlled by DeltaVision softWoRx (Cytiva) using a ×20 0.75 NA UPlanSApo objective lens (Olympus).

Hino M., Iemura K., Ikeda M., Itoh G, & Tanaka K. (2021). Chromosome alignment‐maintaining phosphoprotein CHAMP1 plays a role in cell survival through regulating Mcl‐1 expression. Cancer Science, 112(9), 3711-3721.

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Microscopic Imaging of Live Yeast Cells

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Optical z-sections of live yeast cells were acquired with a Zeiss Axio Imager M2 equipped with a Zeiss Axiocam 506 monochromatic camera, 100× oil-immersion objective (Plan-Apo, NA 1.4), a Zeiss LSM800 equipped with an Airyscan detector, 63× oil-immersion objective (Plan-Apo, NA 1.4) or a Zeiss LSM880 equipped with an Airyscan detector, 63× oil-immersion objective (Plan-Apo, NA 1.4). Widefield images were acquired with ZEN (Carl Zeiss) and processed with Fiji (Schindelin et al., 2012 (link)). Superresolution images were acquired with ZEN (Carl Zeiss) and processed using the automated Airyscan processing algorithm in ZEN (Carl Zeiss) and Fiji. Individual channels of all images were minimally adjusted in Fiji to match the fluorescence intensities between channels for better visualization. Line scan analysis was performed on nonadjusted, single z-sections in Fiji. For Fig. S1 E, yeast were imaged on a DeltaVision MPX microscope (Applied Precision) equipped with a 60× 1.42 NA oil Plan-ApoN or a 100× 1.40 NA oil UplanS-Apo objective lens (Olympus), a multicolor solid state illumination light source, and a CoolSNAP HQ2 camera (Roper Scientific). Acquisition and deconvolution were performed with SoftWoRx software.

English A.M., Schuler M.H., Xiao T., Kornmann B., Shaw J.M, & Hughes A.L. (2020). ER–mitochondria contacts promote mitochondrial-derived compartment biogenesis. The Journal of Cell Biology, 219(12), e202002144.

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Visualizing Mitochondrial Protease Activity

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Cells were grown to mid-log phase in SD–uracil medium for selection of the mitochondrial matrix-targeted CFAse-mcherry plasmid. Mammalian cells were incubated in MEMα medium containing 10% foetal calf serum and 1% penicillin–streptomycin. The expression of the CFAse-mCherry constructs was induced with 1 mM doxycycline overnight. Before the imaging, the medium was exchanged with PBS.
Images were acquired using a DeltaVision MPX microscope (Applied Precision) equipped with a 100× 1.40 NA oil UplanS-Apo objective lens (Olympus), a multicolour illumination light source, and a CoolSNAPHQ2 camera (Roper Scientific). Image acquisition was done at room temperature. Images were deconvolved with Deltavision SoftWoRx software (version 6.5.2) using the manufacturer’s parameters. Images were processed further using FIJI ImageJ (version 1.53c) bundle.

John Peter A.T., Petrungaro C., Peter M, & Kornmann B. (2022). METALIC reveals interorganelle lipid flux in live cells by enzymatic mass tagging. Nature Cell Biology, 24(6), 996-1004.

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Imaging Mitochondrial Matrix-Targeted Proteins

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Cells were grown to mid-log phase in synthetic defined (SD)–uracil medium for selection of the mitochondrial matrix-targeted CFAse-mcherry plasmid. Mammalian cells were incubated in MEMα-medium containing 10 % FCS and 1 % P/S. The expression of the CFAse-mCherry constructs was induced with 1 mM Doxycyline overnight. Prior to the imaging, the medium was exchanged wit PBS.
Images were acquired using a DeltaVision MPX microscope (Applied Precision) equipped with a 100× 1.40 NA oil UplanS-Apo objective lens (Olympus), a multicolor illumination light source, and a CoolSNAPHQ2 camera (Roper Scientific). Image acquisition was done at room temperature. Images were deconvolved with Deltavision SoftWoRx software (version 6.5.2) using the manufacturer’s parameters. Images were processed further using FIJI ImageJ (version 1.53c) bundle.

John Peter A.T., Petrungaro C., Peter M, & Kornmann B. (2022). METALIC reveals interorganelle lipid flux in live cells by enzymatic mass tagging. Nature Cell Biology, 24(6), 996-1004.

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Fluorescence Microscopy Imaging of Yeast Cells

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Cells were grown to mid‐log phase in synthetic dextrose (SD) medium using the appropriate amino acid drop‐out mix for the selection of the plasmids. Cells were supplemented with 10 mM ethanolamine and 10 mM choline for activation of the Kennedy pathway. Images were acquired using a DeltaVision MPX microscope (Applied Precision) equipped with a 100× 1.40 NA oil UplanS‐Apo objective lens (Olympus), a multicolor illumination light source, and a CoolSNAPHQ2 camera (Roper Scientific). Image acquisition was done at RT. Images were deconvolved with SoftWoRx software using the manufacturer's parameters. Images were processed further using the Fiji ImageJ bundle. Z‐projections using MAX intensities are depicted in the figures unless mentioned otherwise.

John Peter A.T., van Schie S.N., Cheung N.J., Michel A.H., Peter M, & Kornmann B. (2022). Rewiring phospholipid biosynthesis reveals resilience to membrane perturbations and uncovers regulators of lipid homeostasis. The EMBO Journal, 41(7), e109998.

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Vacuolar Membrane Staining with FM4-64

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Cells were grown to mid-log phase in synthetic dextrose (SD) medium using the appropriate amino acid drop-out mix for the selection of the plasmids. Cells were supplemented with 10 mM ethanolamine and 10 mM choline for activation of the Kennedy pathway. Images were acquired using a DeltaVision MPX microscope (Applied Precision) equipped with a 100× 1.40 NA oil UplanS-Apo objective lens (Olympus), a multicolor illumination light source, and a CoolSNAPHQ2 camera (Roper Scientific). Image acquisition was done at RT. Images were deconvolved with SoftWoRx software using the manufacturer's parameters. Images were processed further using the FIJI ImageJ bundle. Zprojections using MAX intensities are depicted in the figures unless mentioned otherwise.
FM4-64 staining.
For vacuolar staining, cells were pulse labeled with FM4-64 (Molecular Probes) at a concentration of 5 µg/ml for 20 min in the dark at 30 °C. Cells were washed twice with ice-cold media without FM4-64 and imaged subsequently.

Peter A.T.J., Schie S.v., Cheung N.J., Michel A.H., Peter M., & Kornmann B. (2021). Rewiring phospholipid biosynthesis reveals robustness in membrane homeostasis and uncovers lipid regulatory players.

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