Ir dye labelled anti rabbit and anti mouse igg secondary antibody

A549 human alveolar basal epithelial cells (Cell Repository, NCCS, India) and HEK293T human embryonic kidney cells (ATCC CRL-3216) were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% Fetal Bovine Serum (FBS) and 1% penicillin-streptomycin. Small airway epithelial cells (SAECs; Lonza) were cultured and maintained according to the manufacturer's instruction. Transfection of DNA and Poly(I-C) (InvivoGen) was performed with Lipofectamine 3000 (Invitrogen) in Opti-MEM as per the manufacturer's protocol. Cells were infected in serum-free DMEM with the A/Puerto Rico/8/34 (PR8/H1N1), or NDV Lasota viruses at the MOIs mentioned in the figure legends. After one hour, cells were washed with Phosphate buffered saline (PBS) and replaced with DMEM containing 1% FBS. DMEM, FBS, Opti-MEM, and penicillin-streptomycin were purchased from Invitrogen (Carlsbad, CA, USA).
Anti-FLAG, anti-β actin, and anti-γ Tubulin antibodies were purchased from Sigma Aldrich (St. Louis, MO, USA). Anti-Caspase-3 and Anti-Caspase-1 antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-IFI16 antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). IR dye-labelled anti-Rabbit and anti-Mouse IgG (secondary antibody) were purchased from LI-COR.