Log In Sign up
The largest database of trusted experimental protocols

Taqman 5700 sequence detection system

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The TaqMan 5700 Sequence Detection System is a real-time PCR instrument designed for quantitative gene expression analysis. It utilizes the TaqMan assay technology to detect and quantify target DNA sequences.

Automatically generated - may contain errors

Lab products found in correlation

Gapdh, by Cell Signaling Technology (3 mentions) Fl rage, by Abcam (3 mentions) Rneasy clean up, by Qiagen (2 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions) Sybr green pcr master mix, by Thermo Fisher Scientific (2 mentions) Superscript 3 first strand synthesis system, by Thermo Fisher Scientific (2 mentions) β actin, by Abcam (2 mentions) Percoll gradient, by GE Healthcare (2 mentions) Mg100, by R&D Systems (1 mentions) Ab3611, by Abcam (1 mentions) Ab41548, by Abcam (1 mentions) Hmgb1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

TaqMan system (146 citations) TaqMan detection system (11 citations) Sequence Detection Systems (5 citations)

6 protocols using taqman 5700 sequence detection system

1

Quantifying Molecular Biomarkers in Tissue

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Real-time quantitative PCR (qPCR) was performed with corresponding primers (Supplementary Table S1) using a TaqMan 5700 Sequence Detection System (Applied Biosystems, Foster City, CA) as described (26 (link)).
Western blots were performed as described (26 (link)) using primary antibodies specific for S100B (#ab41548, Abcam, Middlesex, NJ), full length RAGE (FL-RAGE, #ab3611, Abcam), HMGB1, and GAPDH (#2118s, Cell Signaling, USA).
ELISA for mouse IGF-1 (#MG100, R&D Systems, USA) and mouse AGE (#MBS2515909 MyBioSource, USA) were performed according to manufacturer instructions.
Zhang I.Y., Zhou H., Liu H., Zhang L., Gao H., Liu S., Song Y., Alizadeh D., Yin H.H., Pillai R, & Badie B. (2020). Local and Systemic Immune Dysregulation Alters Glioma Growth in Hyperglycemic Mice. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(11), 2740-2753.
+ Open protocol
+ Expand
2

Quantification of MAPK Isoforms via qRT-PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was generated using Trizol reagent (Life Technologies, Carlsbad, CA), followed by double DNase treatment and column purification using Qiagen RNeasy Clean-up (Qiagen, Germantown, MD). The Invitrogen Superscript III First-Strand Synthesis System (Invitrogen, Waltham, MA) was used for cDNA synthesis and TaqMan 5700 Sequence Detection System (Applied Biosystems, Foster City, CA) was used for qRT-PCR. All PCR reactions were run in triplicate. The amplified transcripts were quantified using the comparative ΔCT method as described (Zhang et al., 2007 (link)). cDNA and primers for MAPK11, 12, 13, and 14 and GAPDH (see Supplementary Table S1) were added to SYBR Green PCR Master Mix (SYBR Green I Dye, AmpliTaq DNA polymerase, deoxynucleotides with deoxyuridine triphosphate, and optimal buffer components: Applied Biosystems) and subjected to PCR amplification (1 cycle at 95°C for 3 minutes, 35 cycles at 95°C for 15 seconds and 55°C for 30 seconds, and 1 cycle at 50°C for 2 minutes).
Zhang X.H., Nam S., Wu J., Chen C.H., Liu X., Li H., McKeithan T., Gong Q., Chan W.C., Yin H.H., Yuan Y.C., Pillai R., Querfeld C., Horne D., Chen Y, & Rosen S.T. (2018). Multi-Kinase Inhibitor with Anti-p38γ Activity in Cutaneous T-Cell Lymphoma. The Journal of investigative dermatology, 138(11), 2377-2387.
+ Open protocol
+ Expand
3

Quantitative PCR and Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Real-time quantitative PCR (qPCR) was performed with corresponding primers (Table S1) in a TaqMan 5700 Sequence Detection System (Applied Biosystems, Foster City, CA) as described previously (18 (link)).
Western blots were performed as describe before (18 (link)) using primary antibodies specific for S100B (Abcam), full length RAGE (FL-RAGE) (Abcam), β-actin (Santa Cruse), S100A9 (R&D Systems), HMGB1 and GAPDH (Cell Signaling).
Chen X., Zhang L., Zhang I.Y., Liang J., Wang H., Ouyang M., Wu S., da Fonseca A.C., Weng L., Yamamoto Y., Yamamoto H., Natarajan R, & Badie B. (2014). RAGE Expression in Tumor-associated Macrophages Promotes Angiogenesis in Glioma. Cancer research, 74(24), 7285-7297.
+ Open protocol
+ Expand
4

Quantification of MAPK Isoforms via qRT-PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was generated using Trizol reagent (Life Technologies, Carlsbad, CA), followed by double DNase treatment and column purification using Qiagen RNeasy Clean-up (Qiagen, Germantown, MD). The Invitrogen Superscript III First-Strand Synthesis System (Invitrogen, Waltham, MA) was used for cDNA synthesis and TaqMan 5700 Sequence Detection System (Applied Biosystems, Foster City, CA) was used for qRT-PCR. All PCR reactions were run in triplicate. The amplified transcripts were quantified using the comparative ΔCT method as described (Zhang et al., 2007 (link)). cDNA and primers for MAPK11, 12, 13, and 14 and GAPDH (see Supplementary Table S1) were added to SYBR Green PCR Master Mix (SYBR Green I Dye, AmpliTaq DNA polymerase, deoxynucleotides with deoxyuridine triphosphate, and optimal buffer components: Applied Biosystems) and subjected to PCR amplification (1 cycle at 95°C for 3 minutes, 35 cycles at 95°C for 15 seconds and 55°C for 30 seconds, and 1 cycle at 50°C for 2 minutes).
Zhang X.H., Nam S., Wu J., Chen C.H., Liu X., Li H., McKeithan T., Gong Q., Chan W.C., Yin H.H., Yuan Y.C., Pillai R., Querfeld C., Horne D., Chen Y, & Rosen S.T. (2018). Multi-Kinase Inhibitor with Anti-p38γ Activity in Cutaneous T-Cell Lymphoma. The Journal of investigative dermatology, 138(11), 2377-2387.
+ Open protocol
+ Expand
5

Quantifying Tumor-Associated Leukocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Real-time quantitative PCR (qPCR) was performed with corresponding primers (Supplementary Table 1) in a TaqMan 5700 Sequence Detection System (Applied Biosystems, Foster City, CA) as described previously [17 (link)]. Western blots were performed as describe before [17 (link)] using primary antibodies specific for S100B (Abcam), full length RAGE (FL-RAGE, Abcam), CCL2 (Santa Cruse), β-Actin (Abcam) or GAPDH (Cell Signaling). S100B and soluble RAGE (sRAGE) ELISA were performed according to the manufacturer’s instructions (MyBioSource).
To assess phenotypes of tumor-associated leukocytes, tumor and peritumoral tissue from control or Dul-treated mice were minced and digestion with trypsin for 20 min at 37 °C. Tissue homogenate was then filtered through a 40 μM filter and leukocytes separated by Percoll gradient (GE Healthcare) at 350 g–400 g for 45 min. The isolated cells were characterized by flow and incubated with LPS (0.5 μg/ml, 24 h) to induce cytokine expression prior to analysis.
Gao H., Zhang I.Y., Zhang L., Song Y., Liu S., Ren H., Liu H., Zhou H., Su Y., Yang Y, & Badie B. (2018). S100B suppression alters polarization of infiltrating myeloid-derived cells in gliomas and inhibits tumor growth. Cancer letters, 439, 91-100.
+ Open protocol
+ Expand
6

Quantifying Tumor-Associated Leukocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Real-time quantitative PCR (qPCR) was performed with corresponding primers (Supplementary Table 1) in a TaqMan 5700 Sequence Detection System (Applied Biosystems, Foster City, CA) as described previously [17 (link)]. Western blots were performed as describe before [17 (link)] using primary antibodies specific for S100B (Abcam), full length RAGE (FL-RAGE, Abcam), CCL2 (Santa Cruse), β-Actin (Abcam) or GAPDH (Cell Signaling). S100B and soluble RAGE (sRAGE) ELISA were performed according to the manufacturer’s instructions (MyBioSource).
To assess phenotypes of tumor-associated leukocytes, tumor and peritumoral tissue from control or Dul-treated mice were minced and digestion with trypsin for 20 min at 37 °C. Tissue homogenate was then filtered through a 40 μM filter and leukocytes separated by Percoll gradient (GE Healthcare) at 350 g–400 g for 45 min. The isolated cells were characterized by flow and incubated with LPS (0.5 μg/ml, 24 h) to induce cytokine expression prior to analysis.
Gao H., Zhang I.Y., Zhang L., Song Y., Liu S., Ren H., Liu H., Zhou H., Su Y., Yang Y, & Badie B. (2018). S100B suppression alters polarization of infiltrating myeloid-derived cells in gliomas and inhibits tumor growth. Cancer letters, 439, 91-100.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!