Anti ctgf sc 14939

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-CTGF (sc-14939) is a mouse monoclonal antibody that recognizes the Connective Tissue Growth Factor (CTGF) protein. CTGF is a secreted protein involved in various cellular processes, including cell proliferation, differentiation, adhesion, and extracellular matrix production.

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Lab products found in correlation

Anti cyr61 sc 13100, by Santa Cruz Biotechnology (2 mentions) Anti ki67 gb13030, by Wuhan Servicebio Technology (2 mentions) Xav939 x3004 and iwp 2 i0536, by Merck Group (2 mentions) Anti β actin antibody a5316, by Merck Group (2 mentions) Chir 99021 s2924, by Selleck Chemicals (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Ab31013, by Abcam (1 mentions) Ab186838, by Abcam (1 mentions) Ab92486, by Abcam (1 mentions) Sc 8782, by Santa Cruz Biotechnology (1 mentions) Anti smad2 3 3102, by Cell Signaling Technology (1 mentions) Anti gapdh antibody sc 47724, by Santa Cruz Biotechnology (1 mentions) Gel pro analysis software, by Media Cybernetics (1 mentions) Ab203070, by Abcam (1 mentions) Anti β actin a5441, by Merck Group (1 mentions) Mouse igg 7076, by Cell Signaling Technology (1 mentions) On targetplus non targeting pool sirna, by Horizon Discovery (1 mentions) Rabbit igg 7074, by Cell Signaling Technology (1 mentions) Anti goat igg sc 2354, by Santa Cruz Biotechnology (1 mentions) Monoclonal antibodies against α sma, by Merck Group (1 mentions)

5 protocols using anti ctgf sc 14939

Investigating Hippo and Wnt Pathways

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Antibodies for Phospho-Histone H2A.X (Ser139) (2577), YAP (12395), TAZ (4883), Lats1 (3477), Lats2 (5888), β-catenin (9562), Axin1 (3323), rabbit-IgG-HRP (7074), mouse-IgG-HRP (7076) antibodies are from Cell Signaling Technology. Anti-β-Actin (A5316) antibody is from Sigma-Aldrich. Anti-CTGF (sc-14939) and anti-Cyr61 (sc-13100) antibodies are from Santa Cruz Biotechnology. Anti-Ki67 (GB13030) is from Servicebio. CHIR-99021 (S2924) is from Selleck Chemicals. XAV939 (X3004) and IWP-2 (I0536) is from Sigma-Aldrich.

Zhang L., Cheng F., Wei Y., Zhang L., Guo D., Wang B, & Li W. (2018). Inhibition of TAZ contributes radiation-induced senescence and growth arrest in glioma cells. Oncogene, 38(15), 2788-2799.

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Investigating Hippo and Wnt Pathways

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Zhang L., Cheng F., Wei Y., Zhang L., Guo D., Wang B, & Li W. (2018). Inhibition of TAZ contributes radiation-induced senescence and growth arrest in glioma cells. Oncogene, 38(15), 2788-2799.

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Protein Expression Analysis of TGF-β Signaling

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Total protein was extracted from treated cells using RIPA buffer. Equal amounts of cell extracts were fractionated by SDS-PAGE and analyzed by Western blotting and visualized by enhanced chemiluminescence. The following primary antibodies were employed: antipro-COL1A1 (SC-8782, Santa Cruz, Dallas, TX, USA), anti-TGF-β1 (ab92486, Abcam, Cambridge, MA, USA), anti-TGF-β receptor I antibody (ab31013, Abcam, Cambridge, MA, USA), anti-TGF-β receptor II (ab186838, Abcam, Cambridge, MA USA), antiphospho-Smad2/Smad3 (8828, Cell Signaling, Danvers, MA, USA), anti-Smad2/3 (3102, Cell Signaling, Danvers, MA, USA), anti-CTGF (SC-14939, Santa Cruz, California) and anti-GAPDH antibody (sc-47724, Santa Cruz, Dallas, TX, USA) The quantification of protein bands was carried out using GelPro Analysis Software (Media Cybernetics).

Wang P., Han J., Wei M., Xu Y., Zhang G., Zhang H., Shi L., Liu X., Hamblin M.R, & Wang X. (2018). Remodeling of dermal collagen in photoaged skin using low-dose 5-aminolevulinic acid photodynamic therapy occurs via the transforming growth factor-β pathway. Journal of biophotonics, 11(6), e201700357.

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Investigating BAMBI and CTGF Knockdown in Multiple Myeloma

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ON-TARGETplus Human CTGF siRNA-SMART pool, ON-TARGETplus Human BAMBI siRNA-SMART pool, and ON-TARGETplus non-targeting Pool siRNA were purchased from Dharmacon (Lafayette, CO, USA) and were used for all transfection experiments. BAMBI siRNA(h) #sc-60243, a pool of 3 target-specific siRNAs (Santa Cruz Biotechnology, Dallas, TX, USA) was only used to confirm the effect of knockdown BAMBI expression on MM cell proliferation. The oligonucleotide sequences of the siRNAs in each pool are shown in (Table S1).
An antibody against BAMBI/NMA (ab203070) was purchased from Abcam (Cambridge, UK), anti-CTGF (sc-14939) and anti-goat IgG (sc-2354) from Santa Cruz Biotechnology (Dallas, TX, USA), and antibodies against cyclin D1 (92G2), cyclin D3 (2936), CDK2 (2546), CDK4 (1279), phospho-Smad2 (Ser465/467), phospho-Smad3 (Ser423/425) (C25A9), Smad2/3, rabbit IgG (7074), and mouse IgG (7076) from Cell Signaling Technology (Danvers, MA, USA). Anti-β-actin (A5441) was obtained from Sigma-Aldrich (St. Louis, MO, USA).

Hoang N.T., Hassan G., Suehiro T., Mine Y., Matsuki T, & Fujii M. (2022). BMP and activin membrane-bound inhibitor regulate connective tissue growth factor controlling mesothelioma cell proliferation. BMC Cancer, 22, 984.

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Immunofluorescence Antibody Validation

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Monoclonal antibodies against SNAI-1 (#3895), YAP (#14074) phospho-SMAD2/3 (#8828), SMAD2/3 (#8685), phospho-SMAD3 (#9520) and CAV1 (#3267) were from Cell Signaling Technology; monoclonal antibodies against α-SMA (#A2547) and pan-cytokeratin (#SRE0061) were from Sigma Aldrich (Saint Louis, MO); monoclonal anti GAPDH (#MAB374) was from Millipore (Burlington MA, USA); monoclonal anti-TEF-1 (sc-376113), polyclonal anti-RhoGDI (sc-360) and anti-CTGF (sc-14939) were from Santa Cruz Biotechnology; polyclonal anti-ZO-1 (#617-300) was from Zymed (Invitrogen, Carlsbad, CA); polyclonal anti-FSP1 (#A5114) was from Dako (Glostrup, Denmark); monoclonal anti-calretinin (#ab92341) was from Abcam; anti-MMP2 (#IM33) was from Merck (Darmstadt, Germany); monoclonal anti-human podoplanin (#566456) and FITC Mouse Anti-Human CD45 Clone HI30 (#55548) were from BD Bioscences (Franklin Lakes, NJ); secondary antibodies Alexa Fluor 488, 555, 574 and 594 (Thermofisher Scientific, Massachusetts, USA); Hoechst 33342 and 4,6-diamidino-2-phenylindole (DAPI) were from Invitrogen (Carlsbad, CA). SB431542 was from Selleck (Houston, TX). 1D11.16 anti-TGFβ1 mAb (BE0057) was from inVivoMab/Bio X Cell (Lebanon, NH).

Strippoli R., Sandoval P., Moreno-Vicente R., Rossi L., Battistelli C., Terri M., Pascual-Antón L., Loureiro M., Matteini F., Calvo E., Jiménez-Heffernan J.A., Gómez M.J., Jiménez-Jiménez V., Sánchez-Cabo F., Vázquez J., Tripodi M., López-Cabrera M, & del Pozo M.Á. (2020). Caveolin1 and YAP drive mechanically induced mesothelial to mesenchymal transition and fibrosis. Cell Death & Disease, 11(8), 647.

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