Variant 2 hemoglobin a1c analyzer

Each subject had standardized meal tests at baseline and at the 12-week follow-up visit. Fasting blood samples were collected after an 8-hour overnight fast and postprandial blood was collected 2-hour later. The standard meal test was standardized instant noodles containing 69.3 g of carbohydrates, 9.3 g of protein, and 1.5 g of fat [15 (link)]. Blood pressure, height and waist were measured. BMI = weight (kg)/height(m)².
Plasma glucose including fasting plasma glucose (FPG) and 2-h postprandial plasma glucose (2-h PG) levels were measured by glucose oxidase method. HbA1c was measured by using high-performance liquid chromatography with a VARIANT II Hemoglobin A1c analyzer (Bio-Rad Laboratories, Hercules, CA). Triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), and low-density lipoprotein cholesterol (LDL-c) were determined by applying standard enzymatic methods using a biochemical analyzer (7600–120; Hitachi, Tokyo, Japan). The criteria for the serum sample included: 1) the volume of the serum sample was enough for the assay; 2) serum sample was stored properly. For fasting serum GDF15 levels, 51 of subjects from MET group and 53 subjects from ACA group were measured at the baseline and endpoint by quantitative sandwich enzyme-linked immunosorbent assay (R&D Systems, Minneapolis, USA).