One hour after induction of focal cryolesion, a group of 74 wildtype mice received a single intravenous injection of the specific FXIIa inhibitor rHA-Infestin-4 (CSL Behring GmbH, Marburg, Germany) at a dose of 200 mg/kg body weight [13 (link)]. Control animals received equal volumes of 0.9% sodium chloride (vehicle).
Rha infestin 4
Manufactured by CSL Behring
Sourced in Germany
RHA-Infestin-4 is a laboratory equipment product manufactured by CSL Behring. It is a recombinant human albumin-infestin-4 fusion protein. The core function of this product is to serve as a research tool for in vitro studies.
Automatically generated - may contain errors
3 protocols using rha infestin 4
1
Cryolesion-Induced Neuroinflammation Mitigation
2
FXII Inhibitor and Reconstitution in Murine Brain Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One hour after induction of focal cryolesion or diffuse weight‐drop injury, wild‐type mice received a single intravenous injection of the specific FXII inhibitor recombinant human albumin‐fused infestin‐4 (rHA‐Infestin‐4; CSL Behring, Marburg, Germany) at a dose of 200mg/kg body weight.20 Control animals received equal volumes of 0.9% sodium chloride (vehicle). Intravenous injection of 2μg/g body weight human FXII (hFXII; ADG412H; Sekisui Diagnostics, Lexington, MA), or an hFXII variant that cannot be activated due to modifications in the activation domain (hFXII‐3xAla; CSL Limited, Parkville, Victoria, Australia), administered 1 hour after injury induction and continually every 72 hours, resulted in reconstitution of FXII−/− mice.
3
Evaluating Polyphosphate Effects on Coagulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Kaolin was obtained from Sigma Aldrich. The activated partial thromboplastin time (aPTT) reagent Dade® Actin® FS containing ellagic acid and soybean phospholipids and Pathromtin SL® were purchased from Siemens Healthcare Diagnostics. FXII contact activators STA® - C.K. Prest® and STA® - Cephascreen® were obtained from Diagnostica Stago (Asnières, France), while SynthAFax®, SynthASil® and APTT SP reagent were purchased from IL Werfen (München, Germany). The following synthetic polyphosphates with different lengths were obtained from ICL Pharmaceuticals: ammonium polyphosphate (P42, #2 2846), ammonium polyphosphate (P30, #2 2840), sodium polyphosphate (P75, #7 9990), sodium polyphosphate (P70, #7 1480), sodium polyphosphate (P68, #7 1480). Dornase alfa, the recombinant human DNaseI (Pulmozyme®), was purchased from Roche. FXII purified from human plasma was obtained from Haematologic Technologies. The FXIIa-specific inhibitor rHA-Infestin-4 was supplied by CSL Behring. Thrombin generation assays were performed with the fluorogenic substrate Z-Gly-Gly-Arg-AMC, purchased from Bachem Bubendorf, Switzerland. The thrombin calibrator (α2-macroglobulin-thrombin complex, α2M-T) and MP reagent (containing phosphatidylserine, phosphatidylcholine and phosphatidylethanolamine at a ratio of 20:60:20) were obtained from Diagnostica Stago.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!