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Evos floid cell imaging system

Manufactured by Thermo Fisher Scientific
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The EVOS FLoid cell imaging system is a compact and versatile microscope designed for live-cell imaging and analysis. It features a high-resolution camera, LED illumination, and intuitive software for capturing, processing, and analyzing images of cells in a variety of applications.

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Lab products found in correlation

Live dead cell imaging kit 488 570, by Thermo Fisher Scientific (1 mentions) Synergy htx multi mode microplate reader, by Agilent Technologies (1 mentions) Fitc insulin, by Merck Group (1 mentions)

8 protocols using evos floid cell imaging system

1

Live/Dead Cell Imaging Assay

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Cells were seeded in a 24-well plate overnight. After treatment, cells were stained with the LIVE/DEAD Cell Imaging kit (488/570) (Thermo Fisher Scientific, Inc., Waltham, MA) as described previously [49 (link)]. After incubation at room temperature in the dark, the cells were observed under EVOS FLoid Cell Imaging System (Thermo Fisher Scientific) and measured for live cells (green) with Green-Light Channel and dead cells (red) with Red-Light Channel.
Lyu H., Shen F., Ruan S., Tan C., Zhou J., Thor A.D, & Liu B. (2023). HER3 functions as an effective therapeutic target in triple negative breast cancer to potentiate the antitumor activity of gefitinib and paclitaxel. Cancer Cell International, 23, 204.
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2

Apoptosis Induction in U266 Cells

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U266 cells (at a seeding density of 5 × 103) were treated with GS for 48 h in a 12-well plate. After the treatment time point was completed, caspase-3/caspase-7 green detection reagent was added to the cells, and the cells were incubated for 30 min at 37 °C. The cells were then visualized, and images were captured using the EVOS FLoid cell imaging system (Thermo Fisher Scientific, Waltham, MA, USA).
Akhtar S., Zarif L., Kuttikrishnan S., Prabhu K.S., Patil K., Nisar S., Abou-Saleh H., Merhi M., Dermime S., Bhat A.A, & Uddin S. (2022). Guggulsterone Induces Apoptosis in Multiple Myeloma Cells by Targeting High Mobility Group Box 1 via Janus Activated Kinase/Signal Transducer and Activator of Transcription Pathway. Cancers, 14(22), 5621.
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3

Evaluating Cell Viability with Live/Dead Staining

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U266 cells were seeded at a density of 5 × 103 cells/well in a 6-well plate and treated with GS for 48 h. Live/dead stain was prepared by adding 5 μL of ethidium homodimer-1 (EthD-1) and 5 μL of calcein AM to 10 mL PBS (i.e., final concentrations of 1 μM EthD-1 and 2 μM calcein). The cells were stained with the prepared dye for 30 min and then images were captured using the EVOS FLoid cell imaging system (Thermo Fisher Scientific, Waltham, MA, USA).
Akhtar S., Zarif L., Kuttikrishnan S., Prabhu K.S., Patil K., Nisar S., Abou-Saleh H., Merhi M., Dermime S., Bhat A.A, & Uddin S. (2022). Guggulsterone Induces Apoptosis in Multiple Myeloma Cells by Targeting High Mobility Group Box 1 via Janus Activated Kinase/Signal Transducer and Activator of Transcription Pathway. Cancers, 14(22), 5621.
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4

Doxorubicin Treatment on Cell Viability

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The cells were seeded into a 6-well tissue culture plate and cultured overnight. The media were then replaced with CPBS containing various concentrations of doxorubicin. The plate was incubated at 37°C for 30 min followed by examination with an EVOS Floid cell imaging system (Thermo Fisher, Waltham, MA, USA).
Kauffman M.K., Kauffman M.E., Zhu H., Jia Z, & Li Y.R. (2016). Fluorescence-Based Assays for Measuring Doxorubicin in Biological Systems. Reactive oxygen species (Apex, N.C.), 2(6), 432-439.
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5

Anti-Amoebic Efficacy of Contact Lens Solutions

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Pure contact lens solutions, and NPs at concentrations of 0.25, 0.5, 1.25 and 2.5 p.p.m. conjugated with the contact lens care solutions, were examined in vitro and assessed for their anti-amoebic activity. To determine the anti-amoebic efficacy on trophozoites (log growth phase after 6 days following sub-culturing), the previously described colorimetric 96-well microtitre plate assay, based on the oxido-reduction of alamarBlue, was used [29 (link)]. Subsequently, the plates were analysed over a period of 6 h, 24 h, 48 h, 72 h and 96 h in a Synergy HTX Multimode Microplate Reader (BioTek) using the Gen5 software programme, a test wavelength of 570 nm and a reference wavelength of 630 nm in order to calculate the inhibition curves of the analysis. All experiments were performed three times, in triplicate. Amoebae growth and viability (trophozoite movement and presence of acanthopodia) in both control and tested assays were visualized by an Evos FLoid Cell Imaging System (ThermoFisher).
Hendiger E.B., Padzik M., Żochowska A., Baltaza W., Olędzka G., Zyskowska D., Bluszcz J., Jarzynka S., Chomicz L., Grodzik M., Hendiger J., Piñero J.E., Grobelny J., Ranoszek-Soliwoda K, & Lorenzo-Morales J. (2020). Tannic acid-modified silver nanoparticles enhance the anti-Acanthamoeba activity of three multipurpose contact lens solutions without increasing their cytotoxicity. Parasites & Vectors, 13, 624.
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6

Leukocyte-mediated Insulin Degradation

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To determine whether leukocytes can degrade insulin in vitro, leukocyte subpopulations (PMN, monocytes, and lymphocytes) were cultured with and without the presence of f-Met-Leu-Phe, a chemotactic and leukocyte activating factor. Fluorescent insulin (FITC-insulin; Sigma, St. Louis, MO) at 1:10 diluted in PBS at a volume of 300 uL was added to PMN, monocytes, and lymphocytes in standard sterile 96-well tissue culture-treated polystyrene plates [35 (link), 36 (link)]. Cells were incubated for 2 hours at 37°C in 5% CO2. Immunohistochemistry was used to confirm subpopulations that phagocytosed FITC-insulin. In order to assess cell viability trypan blue and intact nuclei by DAPI staining was utilized. Additional controls included cells without DAPI or insulin-FITC. Following cell staining, plates were gently agitated, incubated for 30 minutes and centrifuged for 5 minutes at 1400 RPM. The supernatant was discarded and replaced with PBS. Cell images were obtained using EVOS Floid cell imaging system (ThermoFisher Scientific, Waltham, MA). Immunohistochemistry was used to confirm subpopulations that phagocytosed FITC-insulin.
Kesserwan S., Lewis B.E., Mao L., Sharafieh R., Atwood T., Kreutzer D.L, & Klueh U. (2022). Inflammation at site of insulin infusion diminishes glycemic control. Journal of pharmaceutical sciences, 111(7), 1952-1961.
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7

Staining Leukemic Cells K562 and U937

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Leukemic cells K562 and U937 were treated with various doses of NSP-B and thiostrepton and the stain was prepared according to the protocol. The cells were stained and visualized by EVOS FLoid Cell Imaging System from Invitrogen (Thermo Fisher Scientific) (19 (link)).
Kuttikrishnan S., Masoodi T., Sher G., Bhat A.A., Patil K., El-Elimat T., Oberlies N.H., Pearce C.J., Haris M., Ahmad A., Alali F.Q, & Uddin S. (2022). Bioinformatics Analysis Reveals FOXM1/BUB1B Signaling Pathway as a Key Target of Neosetophomone B in Human Leukemic Cells: A Gene Network-Based Microarray Analysis. Frontiers in Oncology, 12, 929996.
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8

Multiplex Live/Dead Cell Imaging

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U266 and RPMI8226 cells were treated with escalating doses of NSP‐B, and then incubated for 48 h. The live and dead stain was prepared by adding 5 µL of calcein AM (Component A) and 20 µL of ethidium homodimer‐1 (Component B) to 10 mL of phosphate buffer saline (PBS). The cells were stained with this solution for 15–30 min at room temperature in the dark. The EVOS FLoid Cell Imaging System; Invitrogen (Thermo Fisher Scientific), was used to acquire the images at 20Χ magnification (Kuttikrishnan, Bhat, et al., 2022 (link); Shishodia et al., 2007 (link)).
Kuttikrishnan S., Ahmad F., Mateo J.M., Prabhu K.S., El‐Elimat T., Oberlies N.H., Pearce C.J., Akil A.S., Bhat A.A., Alali F.Q, & Uddin S. (2023). Neosetophomone B induces apoptosis in multiple myeloma cells via targeting of AKT/SKP2 signaling pathway. Cell Biology International, 48(2), 190-200.
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