The interpolation of the experimental curves of the FID was achieved through a non-linear based fitting on the least squares method using the Marquardt algorithm, implemented in SigmaPlot scientific software (version 9, Systat Software Inc., San Jose, CA, USA).
The FID due to the protein and water protons network and water and lipid protons envelope was analyzed by applying two relaxation processes: a Gaussian-type relaxation process related to ‘solid’ polymer protons and a Lorentzian relaxation process related to ‘liquid phase’ protons according to the expfit algorithm [38 (link)] carried out in MatLab version R2009a (The Mathworks Inc., Natick, MA, USA).