Primotech

Manufactured by Zeiss

Sourced in Germany

The Primotech is a versatile lab equipment product from Zeiss. It is designed for efficient and precise sample preparation and processing in a wide range of laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Paraformaldehyde (pfa), by Merck Group (2 mentions) Neutral balsam, by Maclin Biochemical Technology (1 mentions) Haematoxylin h3136, by Merck Group (1 mentions) N861409, by Maclin Biochemical Technology (1 mentions) Tm3000 tabletop microscope, by Hitachi (1 mentions) Matrigel, by Merck Group (1 mentions) Matrigel gel, by BD (1 mentions) Evo ma10, by Zeiss (1 mentions)

6 protocols using primotech

Histopathological Analysis of Rat Liver and Intestine

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After rat liver and intestinal mucosa were fixed in 4% paraformaldehyde (16005, Sigma-Aldrich, St. Louis, MO) for 24 h, they were dehydrated by gradient alcohol, transparentized by xylene (95682, Sigma-Aldrich, St. Louis, MO), and embedded in paraffin (1496904, Sigma-Aldrich, St. Louis, MO). Subsequently, the paraffinized tissues were cut into 5 μm thick sections, dewaxed by xylene and rehydrated by gradient alcohol. Then, the sections were stained with haematoxylin (H3136, Sigma-Aldrich, St. Louis, MO) for 12 min. After being differentiated by hydrochloric alcohol, the sections were stained with eosin (E4009, Sigma-Aldrich, St. Louis, MO) for 5 min. Thereafter, the stained sections were sealed by neutral balsam (N861409, Macklin, Shanghai, China) and dried at 37 °C for 4 h. Afterwards, the histopathological changes in the liver tissues and intestinal mucosa tissues were observed by an optical microscope (ZEISS Primotech, Carl Zeiss, Oberkochen, Germany) under magnifications of ×200 and ×40, respectively.
The morphological changes in liver were determined by NAFLD activity score, which includes histological features and has been defined as unweighted sum of scores for steatosis (0–3), lobular inflammation (0–3) and ballooning (0–2).

Shen S., Wang K., Zhi Y, & Dong Y. (2022). Gypenosides counteract hepatic steatosis and intestinal barrier injury in rats with metabolic associated fatty liver disease by modulating the adenosine monophosphate activated protein kinase and Toll-like receptor 4/nuclear factor kappa B pathways. Pharmaceutical Biology, 60(1), 1949-1959.

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Membrane Particle Contamination Analysis

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Light microscopy of the membranes was used to determine coverage of particle contamination, this was done using Zeiss Primotech microscope (Carl Zeiss Ltd., Cambridge, UK) at 5 x, 10 x and 50 x magnification.
For scanning electronic microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) analysis, a Tabletop Microscope TM3000 was utilized (Hitachi High-Technologies Corporation), samples were mounted on carbon tape and placed in vacuums chamber prior.

Sullivan G.L., Delgado-Gallardo J., Watson T.M, & Sarp S. (2021). An investigation into the leaching of micro and nano particles and chemical pollutants from disposable face masks - linked to the COVID-19 pandemic. Water Research, 196, 117033.

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Matrigel-coated Transwell Invasion Assay

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The upper surface of the bottom membrane of the Transwell chamber was coated with Matrigel (E1270, Sigma‐Aldrich) to assess cell invasion capability. The stained invasive cells were counted under the orthostatic optical microscope (Primotech, CarlZeiss, Oberkochen, Germany) with five visual fields selected randomly.

Yu H., Xu X., Zhu L., Chen S, & He J. (2024). MELK aggravates lung adenocarcinoma by regulating EZH2 ubiquitination and H3K27me3 histone methylation of LATS2. Journal of Cellular and Molecular Medicine, 28(8), e18216.

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Transwell Invasion Assay for AGS and SNU16 Cells

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To assess the invasion of AGS and SNU16 cells, Transwell assay was conducted. 3 × 10⁴ cells in 200 μl serum-free medium were added to the upper chamber precoated with Matrigel gel (catalog no. 354234; BD Biosciences, San Jose, CA, USA). Then, 500 μl of 10% FBS-containing medium was added to the lower chamber as a chemoattractant. Cells were incubated for 48 h at 37°C, and then, cells on the surface of the filter membrane were removed with cotton swabs. Afterwards, the invaded cells were fixed with 4% paraformaldehyde (catalog no. P6148; Sigma-Aldrich) for 30 min, stained with 0.04% crystal violet (catalog no. C0775; Sigma-Aldrich) for 10 min, and counted using an optical microscope (Primotech; Zeiss, Berlin, Germany).

Qi Z., Wang J., Li Y, & Xu Y. (2022). MZF1 Transcriptionally Activated MicroRNA-328-3p Suppresses the Malignancy of Stomach Adenocarcinoma via Inhibiting CD44. Journal of Immunology Research, 2022, 5819295.

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Ash and Filler Particle Morphology

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Image capture with an optical microscope and scanning electron microscopy (SEM) procedures (ASTM E986-04 2017) were performed to evaluate the morphology of both fly and bottom ashes, in comparison to gneissic filler particles, focusing principally on micro-and macro-texture aspects using Zeiss Primotech and Zeiss EVO MA10 models, respectively.

Barra B., Momm L., Guerrero Y., Mikowski A., Clara E., Nguyen M.L, & Hughes G.B. (2021). Evaluation of Technical Feasibility of Reusing Coal Ash in Dense Asphalt Mixes by Assessing Mechanical Performance. Anais da Academia Brasileira de Ciencias, 93(suppl 4).

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Enamel Demineralization by S. mutans Biofilm

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Enamel slabs were exposed a biofilms de S. mutans during 8 h of initial formation under 10% sucrose to observe enamel demineralization (caries-like lesion formation). After the experimental phase, the slabs were washed with 0.9% NaCl to remove the biofilms. An area of the slabs was covered with nail polish as sound enamel control. The slabs were observed at 5x magnification through a polarized light microscope (Primotech, Zeiss, Oberkochen, Germany). This assay was carried out just to portrait the extent of enamel demineralization in an image, and was not done with all the slabs.

Díaz-Garrido N., Lozano C.P., Kreth J, & Giacaman R.A. (2022). Extended biofilm formation time by Streptococcus sanguinis modifies its non-cariogenic behavior, in vitro. Brazilian oral research, 36.

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