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Anti psbd

Manufactured by Agrisera
Sourced in Sweden

Anti-PsbD is a primary antibody product that specifically binds to the D2 protein subunit of Photosystem II (PSII). The D2 protein is an essential component of the PSII reaction center and plays a crucial role in the light-dependent reactions of photosynthesis.

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3 protocols using anti psbd

1

Antibody Production and Validation

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The specific polyclonal anti-S2P2 antibodies were custom produced in rabbits by Davids Biotechnologie GmbH (Germany), using the highly specific synthetically obtained antigen (AA sequence: DNDPDSDIPVDDRNLLKNR). Other primary antibodies (anti-H3, anti-ACT, anti-COXII, anti-TOC75, anti-PsbA, anti-PsbC, anti-PsbD, and anti-Lhcbs) as well as secondary antibodies were purchased from Agrisera AB (Vannas, Sweden).
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2

Protein Expression Analysis in Algae

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Total protein extracted from the fresh algal samples, after determined by a BCA Protein Assay Kit (Beyotime), was run with loading buffer (30 μg) on a 12% SDS-PAGE gel and subsequently transferred to a PVDF membrane, and immunoblotted with anti-PsbD (Agrisera, Sweden), anti-Cyt b6 (Agrisera), antiLhca2 (Agrisera) antibodies. Anti-Histone H3 (Abcam, USA) was selected as the internal reference. After the incubation with an anti-rabbit IgG antibody (BioXCell, USA), the antigen–antibody complexes on the membrane were visualized by using an enhanced chemiluminescence substrate detection kit (Thermo Fisher Scientific, USA) and captured on a ChemiDoc MP imaging system (Bio-Rad, USA).
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3

Antibody Production and Identification

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Anti-EGY2 specific polyclonal antibodies were produced in rabbits by Agrisera AB (Vannas, Sweden) using the highly purified N-terminal region (aa 51-225) of EGY2 from Arabidopsis.
Anti-Lhcb1-6, anti-PsbA, anti-PsbC and anti -PsbD antibodies were purchased from Agrisera AB (Vannas, Sweden).
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