The human OC cell lines SKOV3, IGROV1 and A2780 were from the American
Type Culture Collection (Rockville, MD, USA). De-identified OC ascites samples
were obtained through an IRB approved protocol of the Indiana University Simon
Cancer Center Tissue Bank. Ascites tumor cells were collected by centrifugation
at 200 × g for 3 min. Erythrocytes were lysed by re-suspending the cell
pellet in a 1:4 mixture of cold Hank's balanced salt solution modified (StemCell
Technologies) supplemented with 2% FBS and red blood cell lysis buffer (0.8%
ammonium chloride, 0.1 mM EDTA, pH 7.4) for 5 min. After centrifugation at 350
× g for 5 min, 25,000 ascites derived tumor cells were cultured as
monolayers or spheroids. SKOV3 and primary OC cells were cultured in media
containing 1:1 MCDB 105 (Sigma) and M199 (Cellgro, Herndon, VA, USA)
supplemented with 10% FBS and antibiotics, while IGROV1 and A2780 cells were
grown in RPMI 1640 at 37°, under a humidified atmosphere containing 5%
CO2.
Type Culture Collection (Rockville, MD, USA). De-identified OC ascites samples
were obtained through an IRB approved protocol of the Indiana University Simon
Cancer Center Tissue Bank. Ascites tumor cells were collected by centrifugation
at 200 × g for 3 min. Erythrocytes were lysed by re-suspending the cell
pellet in a 1:4 mixture of cold Hank's balanced salt solution modified (StemCell
Technologies) supplemented with 2% FBS and red blood cell lysis buffer (0.8%
ammonium chloride, 0.1 mM EDTA, pH 7.4) for 5 min. After centrifugation at 350
× g for 5 min, 25,000 ascites derived tumor cells were cultured as
monolayers or spheroids. SKOV3 and primary OC cells were cultured in media
containing 1:1 MCDB 105 (Sigma) and M199 (Cellgro, Herndon, VA, USA)
supplemented with 10% FBS and antibiotics, while IGROV1 and A2780 cells were
grown in RPMI 1640 at 37°, under a humidified atmosphere containing 5%
CO2.