Esi tof biopolymer analysis reference masses

Blank samples included mixtures of methanol/ethanol (1:1) that were prepared in the same way as biological samples. Zomepirac sodium salt (used as the internal standard (IS), arachidonic acid, docosahexaenoic acid, lysophosphatidylethanolamine (LPE) 18:0, phosphatidylethanolamine (PE) 16:0/22:6, LS-MS-grade acetonitrile, methanol, LC-grade formic acid, and ethanol were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low-concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, United States). Purified water was obtained using the Milli-Q Integral three system (Millipore SAS, Molsheim, France) in order to prepare solution A.

Purified water was obtained using the Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). Zomepirac sodium salt (used as an internal standard, IS), L-serine, L-arginine hydrochloride monohydrate, ascorbic acid, L-homoarginine hydrochloride, LC-MS grade methanol acetonitrile, formic acid and LC grade ethanol were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Pure p. a. ammonium solution (25%) was purchased from Avantor Performance Materials (Gliwice, Poland). The API-TOF reference mass solution kit (G1969–850001) and tuning solutions, ESI-L low concentration tuning mix (G1969–85000) and ESI-TOF Biopolymer Analysis reference masses (G1969–850003) were purchased from Agilent Technologies (Santa Clara, California, United States).

Purified water was obtained using the Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). Zomepirac sodium salt (used as internal standard, IS), LS-MS-grade acetonitrile (ACN), methanol (MeOH), and formic acid (FA) were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Pure p.a. ammonium solution (25%) was purchased from Avantor Performance Materials (Gliwice, Poland). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, USA). Standards to confirm the identity of metabolites were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) (taurine, pipecolic acid, leucine, histidine, phenylalanine, 3-methylhistidine, citric acid, tryptophan, adenosine, and stearoylcarnitine) and Avanti Polar Lipids Inc. (AL, USA) (LPI 18:0 and LPG 18:1).