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Anti α tubulin monoclonal antibodies

Manufactured by Thermo Fisher Scientific
Sourced in United States

Anti-α-tubulin monoclonal antibodies are reagents used to detect and study the α-tubulin protein, a key component of the cytoskeleton in eukaryotic cells. These antibodies can be used in various immunoassay techniques, such as Western blotting, immunocytochemistry, and immunohistochemistry, to visualize and analyze the distribution and expression of α-tubulin in different cell types and tissues.

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4 protocols using anti α tubulin monoclonal antibodies

1

Western Blot Analysis of Inflammatory Signaling

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Fetal bovine serum (FBS), Dulbecco’s modified Eagle medium (DMEM), L-glutamine penicillin/streptomycin, and anti-α-tubulin monoclonal antibodies (mAbs) were purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). LPS (Escherichia coli 0127:B8), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), and 4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-1H-imidazole (SB203580) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-Lamin B1 and anti-iNOS polycloncal antibody (pAb) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The anti-TNF-α, anti-JNK, anti-phospho-c-JNK (Thr183/Tyr185), anti-phospho-p44/p42 ERK (Thr202/Tyr204), anti-phospho-p38 MAPK (Thr180/Tyr182) pAbs, and anti-phospho-p65 (Ser536), anti-p65, anti-IκBα, anti-ERK and anti-p38 MAPK mAbs were purchased from Cell Signaling (Danvers, MA, USA). Anti-IL-1β pAb was purchased from BioVision (Milpitas, CA, USA). Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit immunoglobulin G (IgG), and sheep anti-mouse IgG were purchased from Amersham (Buckinghamshire, UK). The Western blotting detection reagent of enhanced chemiluminescence (ECL) and Hybond™-P polyvinylidene difluoride (PVDF) blotting membranes were purchased from GE Healthcare Life Sciences (Waukesha, WI, USA).
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2

Cellular Response to Inflammatory Stimuli

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Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), L-glutamine/penicillin/streptomycin, and anti-α-tubulin monoclonal antibodies (mAbs) were purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). LPS and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were bought from Sigma-Aldrich (St. Louis, MO, USA). Anti-iNOS, COX2, and Nrf2 polyclonal antibodies (pAbs) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). An anti-HO-1 pAb was purchased from Enzo (Farmingdale, New York, NY, USA). The anti-phospho-p65 (Ser536) mAb was purchased from Cell Signaling (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit immunoglobulin G (IgG) and sheep anti-mouse IgG were purchased from Amersham (Buckinghamshire, U.K.). Enhanced chemiluminescence (ECL) Western blotting detection reagent and Hybond-P polyvinylidene difluoride (PVDF) blotting membranes were purchased from GE Healthcare Life Sciences (Waukesha, WI, USA).
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3

Esculetin and ML385 Modulation of Cellular Pathways

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Esculetin (≥98%) and ML385 were obtained from Cayman (Ann Arbor, MI, USA). Dimethyl sulfoxide (DMSO), lipoteichoic acid (LTA), N-acetyl L-cysteine (NAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma (St. Louis, MO, USA). Anti-iNOS (sc-650) and polyclonal antibody (pAb) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The anti-phospho-c-JNK (Thr183/Tyr185, 9251), anti-phospho-p44/p42 ERK (Thr202/Tyr204, 9101) and anti-phospho-p38 MAPK (Thr180/Tyr182, 9211) pAbs, and anti-phospho-p65 (Ser536, 3033), anti-p65 (4764) and anti-IκBα (4812) mAbs were purchased from Cell Signaling (Danvers, MA, USA). Anti-Nrf2 (GTX103322) pAbs was purchased from GeneTex (Irvine, CA, USA). Fetal bovine serum (FBS), Dulbecco’s modified Eagle medium (DMEM), L-glutamine penicillin/streptomycin, and anti-α-tubulin monoclonal antibodies (mAbs) were purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). Amersham (Buckinghamshire, UK) supplied horseradish peroxidase (HRP)-conjugated donkey anti-rabbit immunoglobulin G (IgG) and sheep anti-mouse IgG.
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4

Investigating Anti-Inflammatory Signaling Pathways

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Fetal bovine serum (FBS), Dulbecco’s modified Eagle medium (DMEM), l-glutamine penicillin/streptomycin, and anti-α-tubulin monoclonal antibodies (mAbs) were purchased from Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). LPS (Escherichia coli 0127:B8), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-Lamin B1 and anti-iNOS polycloncal antibody (pAb) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The anti-TNF-α, anti-phospho-c-JNK (Thr183/Tyr185), anti-phospho-p44/p42 ERK (Thr202/Tyr204), anti-phospho-p38 MAPK (Thr180/Tyr182) pAbs, anti-phospho-p65 (Ser536), anti-phospho-Src family (Tyr416), and anti-phospho-focal adhesion kinase (FAK) (Tyr397) mAbs were purchased from Cell Signaling (Danvers, MA, USA). Anti-IL-1β pAb was purchased from BioVision (Milpitas, CA, USA). Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit immunoglobulin G (IgG), and sheep anti-mouse IgG were purchased from Amersham (Buckinghamshire, UK). The Western blotting detection reagent of enhanced chemiluminescence (ECL) and Hybond™-P polyvinylidene difluoride (PVDF) blotting membranes were purchased from GE Healthcare Life Sciences (Waukesha, WI, USA).
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