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Pbs tween

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PBS Tween is a buffered saline solution containing the detergent Tween 20. It is commonly used in various laboratory techniques, such as immunoassays and Western blotting, to facilitate the washing and blocking steps. The primary function of PBS Tween is to provide a controlled, consistent buffer environment for these applications.

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Lab products found in correlation

Sc 12462 r, by Santa Cruz Biotechnology (2 mentions) Odyssey clx imaging system, by LI COR (2 mentions) Sc 58414, by Santa Cruz Biotechnology (2 mentions) Nb100 64754, by Novus Biologicals (2 mentions) Rsv glycoprotein g, by Bio-Rad (2 mentions) Wbkls0500, by Merck Group (2 mentions) Ab7046, by Abcam (1 mentions)

Close spelling variants of this product

PBS Tween-20 (5 citations) PBS+0.2% tween-20 (2 citations)

2 protocols using pbs tween

1

Exosomal Viral Antigen Detection

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Immunoblot was used to detect SAgs, 20S proteasome, and viral antigens in exosomes from LTxRs diagnosed with RVI and from stable controls. Total exosome protein (3 µg) was resolved in polyacrylamide gel electrophoresis, and the proteins were transferred into a polyvinylidene difluoride membrane. The membrane was blocked with 5% non-fat milk in 1x phosphate buffered saline and was probed with exosome-specific marker CD9 (312102, BioLegend), Col-V (ab7046, Abcam, Cambridge, United Kingdom), and Kα1T (sc-12462-R, Santa Cruz Biotechnology, Dallas, TX). 20S proteasome subunit α3 (sc-58414, Santa Cruz Biotechnology), rhinovirus VP3 (MA5-18249, Thermo Fisher Scientific, Waltham, MA), coronavirus (NB100-64754, Novus Biologicals, Littleton, CO), and RSV glycoprotein G (7950-0980, Bio-Rad Laboratories, Hercules, CA) were used as primary Abs; secondary Abs conjugated with horseradish peroxidase (HRP) were used specific to primary Ab. The blots were washed with PBS Tween (Thermo Fisher Scientific), developed using chemiluminescent HRP substrate (WBKLS0500, MilliporeSigma, Burlington, MA), and exposed using Odyssey CLx Imaging System (LI-COR Biosciences, Lincoln, NE). The band intensity of target protein was quantified using ImageJ software and normalized with CD9.
Gunasekaran M., Bansal S., Ravichandran R., Sharma M., Perincheri S., Rodriguez F., Hachem R., Fisher C.E., Limaye A.P., Omar A., Smith M.A., Bremner R.M, & Mohanakumar T. (2020). Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection. The Journal of Heart and Lung Transplantation, 39(4), 379-388.
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2

Exosomal Biomarkers for Respiratory Viral Infections

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Immunoblot was used to detect SAgs, 20S proteasome, and viral antigens in exosomes from LTxRs diagnosed with RVI and from stable controls. Total exosome protein (3μg) was resolved in polyacrylamide gel electrophoresis, and the proteins were transferred into a polyvinylidene difluoride membrane. The membrane was blocked with 5% nonfat milk in 1X PBS and was probed with exosome-specific marker CD9 (312102, Biolegend, SanDiego, CA), Col-V (ab7046, Abcam), and Kα1T (sc-12462-R, SantaCruz Biotechnology, Dallas, TX). 20S proteasome subunit α3 (sc-58414, SantaCruz Biotechnology), rhinovirus VP3 (MA5–18249, Thermo Fisher Scientific, Waltham, MA), coronavirus (NB100–64754, Novus Biologicals, Littleton, CO), RSV Glycoprotein G (7950–0980, Bio-Rad Laboratories, Hercules, CA) were used as primary Abs; secondary Abs conjugated with HRP were used specific to primary Ab. The blots were washed with PBS Tween (Thermo Fisher Scientific), developed using chemiluminescent HRP substrate (WBKLS0500, MilliporeSigma), and exposed using Odyssey CLx Imaging System (LI-COR Biosciences, Lincoln, NE). The band intensity of target protein was quantified using ImageJ software and normalized with CD9.
Gunasekaran M., Bansal S., Ravichandran R., Sharma M., Perincheri S., Rodriguez F., Hachem R., Fisher C.E., Limaye A.P., Omar A., Smith M.A., Bremner R.M, & Mohanakumar T. (2020). Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection. The Journal of Heart and Lung Transplantation, 39(4), 379-388.
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