Np keyhole limpet hemocyanin

Manufactured by Biosearch Technologies

NP)-Keyhole Limpet Hemocyanin (KLH) is a high-molecular-weight, copper-containing respiratory protein isolated from the hemolymph of the giant keyhole limpet (Megathura crenulata). It is commonly used as a carrier protein in conjugate vaccines and immunoassays.

Automatically generated - may contain errors

Lab products found in correlation

Imject alum, by Thermo Fisher Scientific (1 mentions) Np ficoll, by Biosearch Technologies (1 mentions)

Spelling variants of this product

Keyhole Limpet Hemocyanin (NP-KLH) (4 citations) NP-32 Keyhole Limpet Hemocyanin (3 citations)

3 protocols using np keyhole limpet hemocyanin

Induction of Germinal Centers in iLNs

Check if the same lab product or an alternative is used in the 5 most similar protocols

To induce GCs in iLNs, recipient C57BL/6 mice were immunised subcutaneously on both flanks on the lower part of the body with NP-KLH (4-hydroxy-3-nitrophenylacetyl (NP)-Keyhole Limpet Hemocyanin (KLH), #N-5060-25 Biosearch Technologies). NP-KLH was first diluted in PBS and the same volume of Imject™ Alum (ThermoScientific #77161) was added to reach a final concentration of 0.5mg/mL NP- KLH. After 30 min of vortexing, 100μL of emulsion were injected subcutaneously (s.c.) into the hind flanks of the experimental mice, which have received the intravenous transfer of donor B cells.

Munro J., Nicholl J., O'Cathain A, & Knowles E. (2000). Impact of NHS Direct on demand for immediate care: observational study. BMJ : British Medical Journal, 321(7254), 150-153.

+ Open protocol

+ Expand

Immunization of Conditional Knockout Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Six to 8 week old ELL2 conditional knockout mice and litter mate control mice were immunized i.p. with either 4-hydroxy-3-nitrophenyl acetyl (NP)-Ficoll (F1420, Biosearch technologies) at 25ug in 0.1ml PBS or 100 ug NP-keyhole limpet hemocyanin (KLH) (Biosearch technologies N-5060) precipitated with alum (Pierce, 77161) as previously described for blimp knockouts (19 (link)). Serum was collected at 1, 2 and 3 weeks post-injection using NP-BSA coated plates in an ELISA. For the recall response the same dose of NP-KLH was given 6 weeks after the initial dose and serum collected at 7 and 14 days.

Park K.S., Bayles I., Szlachta-McGinn A., Paul J., Boiko J., Santos P., Liu J., Wang Z., Borghesi L, & Milcarek C. (2014). Transcription Elongation Factor ELL2 Drives Ig Secretory-Specific mRNA Production and the Unfolded Protein Response. The Journal of Immunology Author Choice, 193(9), 4663-4674.

+ Open protocol

+ Expand

NP Immunization in Genetically Modified Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wild-type (wt), IDO1 ko, IDO2 ko, or dko C57BL/6 mice were immunized i.p. with 50 μg (4-Hydroxy-3-Nitrophenyl)Acetyl (NP)-Ficoll, 50 μg NP-LPS, or 100 μg NP-keyhole limpet hemocyanin (KLH), precipitated in alum (Biosearch Technologies). NP-KLH immunized mice were boosted 21 days after initial immunization with 100 μg NP-KLH precipitated in alum. NP-Ficoll and NP-LPS immunized mice were bled on day 7 and NP-KLH mice were bled on day 10 after primary and secondary immunizations. NP titers were measured by ELISA.

Merlo L.M., DuHadaway J.B., Montgomery J.D., Peng W.D., Murray P.J., Prendergast G.C., Caton A.J., Muller A.J, & Mandik-Nayak L. (2020). Differential Roles of IDO1 and IDO2 in T and B Cell Inflammatory Immune Responses. Frontiers in Immunology, 11, 1861.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!