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Genesnap visualisation program

Manufactured by Syngene
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GeneSnap is a software program designed for the visualization and analysis of gel electrophoresis images. The core function of GeneSnap is to capture, digitize, and analyze images of DNA, RNA, or protein samples separated by gel electrophoresis. The program provides tools for image capture, lane detection, band quantification, and molecular weight analysis.

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Lab products found in correlation

Taq polymerase, by Thermo Fisher Scientific (2 mentions) Qiaamp dna blood mini kit, by Qiagen (2 mentions) Dna engine dyad thermal cycler, by Bio-Rad (2 mentions) Genegenius bioimaging system, by Syngene (1 mentions) Bio imaging system, by Syngene (1 mentions)

Close spelling variants of this product

GeneSnap (72 citations) GeneSnap program (3 citations)

2 protocols using genesnap visualisation program

1

PCR Analysis of Pancreatic Tumor DNA

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PCR was conducted using TAQ polymerase from Invitrogen (1034020) as per manufacturer’s instructions. DNA for reactions was isolated from pancreatic tumours using the QiaAmp DNA Blood Mini Kit (Qiagen) as per manufacturer’s instructions. 0.1 ng of DNA was used in each reaction together with a combination of 0.5 mM oligo1 and either oligo 2 or oligo 3 primers, as per figure legend, and 0.5 mM β-actin primers as control. A Bio-Rad DNA Engine DYAD Thermal Cycler was used with the following cycling parameters 95 °C 10 min, 25 cycles of 95 °C 30 s, 60 °C 30 s, 72 °C 30 s and 72 °C 10 min. Samples were loaded in a polyacrylamide gel and visualised with Sybr Safe as per manufacturer’s instructions using a Gene Genius Bioimaging system with a Syngene GeneSnap visualisation program.
Liko D., Mitchell L., Campbell K.J., Ridgway R.A., Jones C., Dudek K., King A., Bryson S., Stevenson D., Blyth K., Strathdee D., Morton J.P., Bird T.G., Knight J.R., Willis A.E, & Sansom O.J. (2019). Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas. Cell Death and Differentiation, 26(12), 2535-2550.
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2

PCR Amplification and Gel Visualization

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PCR was conducted using TAQ polymerase from Invitrogen (1034020) as per manufacturer’s instructions. DNA for reactions was isolated from pancreatic tumours using the QiaAmp DNA Blood Mini Kit (Qiagen) as per manufacturer’s instructions. 0.1 ng of DNA was used in each reaction together with a combination of 0.5 mM oligo1 and either oligo 2 or oligo 3 primers, as per figure legend, and 0.5 mM β-actin primers as control. A Bio-Rad DNA Engine DYAD Thermal Cycler was used with the following cycling parameters 95 °C 10 min, 25 cycles of 95 °C 30 s, 60 °C 30 s, 72 °C 30 s and 72 °C 10 min. Samples were loaded in a polyacrylamide gel and visualised with Sybr Safe as per manufacturer’s instructions using a Gene Genius Bioimaging system with a Syngene GeneSnap visualisation program.
Liko D., Mitchell L., Campbell K.J., Ridgway R.A., Jones C., Dudek K., King A., Bryson S., Stevenson D., Blyth K., Strathdee D., Morton J.P., Bird T.G., Knight J.R., Willis A.E, & Sansom O.J. (2019). Brf1 loss and not overexpression disrupts tissues homeostasis in the intestine, liver and pancreas. Cell Death and Differentiation, 26(12), 2535-2550.
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