AOM (A5486), hemin (51280), EGCG (E4143), trigonelline hydrochloride (T5506), and MitoTempo (SML 0737) were obtained from Sigma-Aldrich, Inc. (St. Louis, MO, USA). DSS with a molecular weight of 36,000–50,000 was obtained from MP Biomedical Inc. (Santa Ana, CA, USA, MFCD00081551). The colorectal cancer cell line, Caco2, was purchased from the Korean Cell Line Bank (Seoul, Korea). Fetal bovine serum (FBS), phosphate-buffered solution (PBS), and high glucose Dulbecco’s Modified Eagle Medium (DMEM) were purchased from HyClone (Logan, UT, USA). Penicillin-streptomycin solution, cell culture dishes, and plates were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). CDK2 (sc-6248), CDK4 (sc-23896), cyclin D (sc-8396), cyclin E (sc-247), and β-actin (sc-47778) primary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Nrf2 (NBP1-32822) and Keap1 (NBP2-03319) antibodies were purchased from Novus Biologicals (Centennial, CO, USA). Horseradish peroxidase-conjugated mouse anti-rabbit secondary antibody and -conjugated mouse IgG kappa binding protein were purchased from Santa Cruz Biotechnology. All mRNA primers were purchased from Cosmogenetech (Seoul, Korea).
Nrf2 nbp1 32822
Manufactured by Novus Biologicals
Sourced in United States
Nrf2 (NBP1-32822) is a primary antibody product from Novus Biologicals. It is used for the detection of Nrf2 (Nuclear factor erythroid 2-related factor 2) protein in various sample types.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase conjugated mouse anti rabbit secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Cell culture dishes, by Thermo Fisher Scientific (1 mentions)
β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
Mitotempo sml0737, by Merck Group (1 mentions)
Cyclin e sc 247, by Santa Cruz Biotechnology (1 mentions)
Caco 2, by Korean Cell Line Bank (1 mentions)
Imagequant, by GE Healthcare (1 mentions)
Gapdh sc 47724, by Santa Cruz Biotechnology (1 mentions)
Hmox1, by Cell Signaling Technology (1 mentions)
Keap1, by Novus Biologicals (1 mentions)
Lamin b1, by Cell Signaling Technology (1 mentions)
Imagej, by GE Healthcare (1 mentions)
2 protocols using nrf2 nbp1 32822
1
Colorectal Cancer Cell Line Caco2 Study
2
Western Blot Analysis of Oxidative Stress Regulators
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Protein expression was determined by western blot analysis. Equal amount of protein from each sample was run in Tris-glycine SDS-PAGE gel, followed by transfer to PVDF membrane. After blocking the membrane with 5% milk for 1 h at room temperature, the membrane was incubated further for 2 h with antibodies specific for target proteins: NRF2 (NBP1-32822, 1/1000 dilution), pNRF2 (S40) (NB100-80012, 1/1000 dilution) from Novus Biologicals (Littleton, CO); KEAP1 (4617, 1/500 dilution), HMOX1 (5061, 1/500 dilution), NQO1 (3187, 1/1000 dilution), HIF1Aα (3716, 1/1000 dilution), HSF1 (4356, 1/1000 dilution), and lamin B1 (12586, 1/1000 dilution) from Cell Signaling (Danvers, MA); GAPDH (sc-47724, 1/5000 dilution) from Santa Cruz (Dallas, TX); β-Actin (MA5-15739-HRP, 1/2000 dilution) from Thermo Fisher (Waltham, MA) and GCLC (ab190685, 1/1000 dilution), GCLM (ab124827, 1/1000 dilution), and TXN (ab26320, 1/1000 dilution) from Abcam (Cambridge, MA). The membrane was subsequently incubated with species-specific HRP-conjugated secondary antibody followed by incubation with chemiluminescence substrate and imaging. The band intensity of each of the target proteins was quantified using either ImageQuant or Image J software (GE Healthcare, Sweden).
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