Bhk 21 cells

The LKR10 cell line (kind gift from Guido Bommer, de Duve Institute, Brussels, Belgium) is derived from lung adenocarcinoma tissues from a K-rasLA1 mouse (Johnson and others 2001 (link)). A549 cells (ATCC) were kindly provided by Pierre Coulie, BALB/3T3 fibroblasts (Aaronson and Todaro 1968 (link)) by Francis Brasseur (Ludwig Institute for Cancer Research, Brussels, Belgium), and Hela-M (Dong and others 2001 (link)) by Robert H. Silverman (Lerner Research Institute, Cleveland).
Those cells, and derivatives, were maintained in Dulbecco's modified Eagle's medium (DMEM) (Lonza, Vervier, Belgium) containing 4.5 g/L glucose, supplemented with 10% fetal calf serum (FCS) (Sigma-Aldrich, Overijse, Belgium). African green monkey kidney (Vero) cells (ATCC) were cultured in DMEM supplemented with 10% FCS, nonessential amino-acid, l-Glutamine, and sodium pyruvate. BHK-21 cells (ATCC) were cultured in Glasgow's minimum essential medium (Gibco; Thermo Fisher Scientific, Asse, Belgium) supplemented with 10% newborn calf serum and 2.95 g/L tryptose phosphate broth. All media were supplemented with 50 U/mL penicillin and 50 μg/mL streptomycin (Lonza).

Vero (ATCC CCL81), HEK-293 (ATCC CRL1573) and HEK-293T cells (ATCC CRL3216) were maintained at 37°C, 5% CO₂ in Dulbecco Modified Eagle’s Medium (DMEM, Thermo Fisher scientific, Montigny-le-Bretonneux, France) supplemented with 5% fetal bovine serum (FBS, Lonza), 1 mM sodium pyruvate, penicillin (1 U/mL)/streptomycin (1 μg/mL) and 2 mM L-glutamine. BHK-21 cells (ATCC CCL10) were maintained at 37°C in Dulbecco Modified Eagle’s Medium (DMEM, Thermo Fisher scientific) supplemented with 10% fetal bovine serum (FBS, Lonza, France) and penicillin (1 U/mL)/streptomycin (1 μg/mL). C6/36 cells (ATCC CRL1660) were maintained at 28°C, in Leibowitz L-15 Medium supplemented with 1 mM sodium pyruvate, penicillin (1 U/mL)/streptomycin (1 μg/mL), 1 mL non-essential amino acids, and 2 mM L-glutamine (Thermo Fisher Scientific, France).