(See SI for description of all assays.) Reaction volumes of 100 µL were used in Microfluor1 black flat bottom microtiter 96 well plates (Thermo Scientific). 50 µL of L. plantarum lysate (3000, 1500, 750, 375, 188, 94, 47 μg/ml) in buffer (0.1 M sodium phosphate, pH = 6) was added to each well. A 50 µL solution of probe (300 µM) in buffer with 5% DMSO was then added to initiate the reaction. Reactions were immediately placed in a plate reader (SPECTRAmax Gemini XS) pre-warmed to 37 °C, and reaction progress was monitored at 450 nm (excitation 350 nm) for 20 minutes. Reactions had final concentrations of 150 μM probe, 0.1 M sodium phosphate (pH 6), and 2.5% DMSO.
Microfluor1 black flat bottom microtiter 96 well plates
Manufactured by Thermo Fisher Scientific
The Microfluor1 black flat bottom microtiter 96 well plates are a type of lab equipment used in various experimental procedures. They provide a standardized and consistent platform for conducting assays and experiments that require a 96-well format. The plates have a flat bottom design and are made of a black material, which can be beneficial for certain fluorescence-based applications.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using microfluor1 black flat bottom microtiter 96 well plates
1
Enzymatic Activity Quantification of L. plantarum
2
Enzyme Kinetics Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(See SI for description of all assays) Reaction volumes of 100 µL were used in Microfluor1 black flat bottom microtiter 96 well plates (Thermo Scientific). 50 μl BSH (80, 40, 20, 10, 5, 2.5, 1.3, 0 µg/ml) in 0.1 M sodium phosphate buffer (pH = 6) were added to the appropriate wells. A 50 µL solution of probe (300 µM) in buffer with 5% DMSO was then added to initiate the reaction. Reactions were immediately placed in a plate reader (SPECTRAmax Gemini XS) pre-warmed to 37 °C, and reaction progress was monitored at 450 nm (excitation 350 nm) for 20 minutes. Reactions had final concentrations of 150 μM probe and 2.5% DMSO.
3
Bacterial Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(See SI for description of all assays.) A starter culture was created by inoculation of 5 ml MRS broth from a L. plantarum colony grown on MRS agar. The starter culture was incubated overnight at 37 °C overnight. The 5 ml starter culture was then added to 95 ml fresh MRS broth and grown until the optical density at 600 nm reached 0.7. At that point, the cells were pelleted via centrifugation. The cell pellet was resuspended in 15 ml PBS, and centrifuged. The cells were additionally washed with PBS and pelleted once more. The resulting pellet was suspended in 380 μl PBS. The cell suspension was then serially diluted two-fold.
50 μl of the cell suspension was added to Microfluor1 black flat bottom microtiter 96 well plates (Thermo Scientific). A 50 µL solution of probe (300 µM) in buffer with 5% DMSO was then added to initiate the reaction. Reactions were immediately placed in a plate reader (SPECTRAmax Gemini XS) pre-warmed to 37 °C, and reaction progress was monitored at 450 nm (excitation 350 nm) for 60 minutes. Reactions had final concentrations of 150 μM probe and 2.5% DMSO.
50 μl of the cell suspension was added to Microfluor1 black flat bottom microtiter 96 well plates (Thermo Scientific). A 50 µL solution of probe (300 µM) in buffer with 5% DMSO was then added to initiate the reaction. Reactions were immediately placed in a plate reader (SPECTRAmax Gemini XS) pre-warmed to 37 °C, and reaction progress was monitored at 450 nm (excitation 350 nm) for 60 minutes. Reactions had final concentrations of 150 μM probe and 2.5% DMSO.
4
Microbiome Lysate BSH Activity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reaction volumes of 100 µL were used in Microfluor1 black flat bottom microtiter 96 well plates (Thermo Scientific). 50 μl of gut microbiome lysate (1 mg/ml) with BSH (40 µg/ml) or buffer were added to the appropriate wells. A 50 µL solution of probe (300 µM) in buffer with 5% DMSO was then added to initiate the reaction. Reactions were immediately placed in a plate reader (SPECTRAmax Gemini XS) pre-warmed to 37 °C, and reaction progress was monitored at 450 nm (excitation 350 nm) for 20 minutes. Reactions had final concentrations of 20 µg/ml BSH, 500 µg/ml lysate protein, 150 μM probe and 2.5% DMSO.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!