Anti gapdh polyclonal antibodies

Kidney tissues were homogenized and lysed in RIPA lysis buffer (Bioteke Corporation, China). Cells were lysed in RIPA lysis buffer directly. After centrifugation, supernatants were collected and protein concentrations were detected using a BCA protein concentration detection kit (Beyotime, China). Equal amounts of protein were separated by 7.5–12.5% SDS-polyacrylamide gel electrophoresis (PAGE) (Beyotime, China) and transferred onto PVDF membranes. The membranes were blocked with 2% BSA for 1 h at room temperature and incubated with primary antibodies at 4°C overnight. The primary antibodies used were as follows: anti-SRC (phosphor Y529) antibodies (Abcam, 1 : 10000), mouse B7-1/CD80 affinity purified polyclonal antibodies (R&D system, 1 μg/ml), and anti-GAPDH polyclonal antibodies (Proteintech, 1 : 20000). Blots were then washed and incubated with peroxidase-conjugated affinipure goat anti-rabbit or rabbit anti-goat secondary antibodies (IgG-HRP; ZSGB-BIO, Beijing, China, 1 : 5000) at room temperature for 1 h. After several washes, visualization was performed on an ECL Western blotting detection system (GE healthcare; RPN2106).

Biotinylated lectins, LCA, SNA, PHA-E, PHA-L, and AAL (Supplementary Table 1), were purchased from Vector Laboratories Inc. (Burlingame, CA, USA). Horseradish-peroxidase (HRP)-conjugated Affinipure Donkey anti-human IgG, rabbit anti-EFF1A2 and anti-GAPDH polyclonal antibodies were purchased from Proteintech Group (Chicago, IL, USA); rabbit anti-CFTR polyclonal antibody was from Alomone Labs (Jerusalem, Israel); rabbit anti-HIF1α polyclonal antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA); rabbit anti-HP polyclonal antibody and rabbit anti-AFP polyclonal antibody were purchased from Boster (Wuhan, China); rabbit anti-mouse α2,6-sialyltransferase Affinity-Purify antibody was obtained from Immuno Biological Laboratories Co, Ltd. (Japan). Horseradish peroxidase-labelled anti-rabbit IgG antibody was obtained from Beyotime (Shanghai, China), deferoxamine mesylate salt (DFO) was purchased from Sigma (St. Louis, MO, USA).