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Mal peg sva

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MAL-PEG-SVA is a heterobifunctional polyethylene glycol (PEG) reagent that contains a maleimide (MAL) group and a succinimidyl valerate (SVA) group. The MAL group can react with thiol-containing compounds, while the SVA group can react with primary amines. This reagent is commonly used for the conjugation of proteins, peptides, or other molecules with thiol and amine functionalities.

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Lab products found in correlation

Mpeg sva, by Laysan Bio (3 mentions) β mercaptoethanol, by Merck Group (2 mentions) 1 3 aminopropyl silatrane aps, by Merck Group (1 mentions) Dbco peg4 nhs, by Merck Group (1 mentions) 1 1 1 3 3 3 hexafluoroisopropanol hfip, by Merck Group (1 mentions)

5 protocols using mal peg sva

1

Antibody-conjugated Cellulose Nanowhiskers

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CL-NWs were aminated by adding different concentrations of ammonium hydroxide at 4°C overnight to form CL-NWs-NH2 and dialyzed for 24 h to remove free NH4OH. For the Ab conjugation, MAL-PEG-SVA (MAL-PEG-SVA, Laysan Bio) was reacted at excess with CL-NWs-NH2 at RT for 30 min to form CL-NWs-PEG-MAL or CL-NWs-PEG. Anti-HER2 Ab Herceptin® was reacted in the last step with MAL-PEG to form CL-NWs-PEG-Ab. NWs were purified by ultracentrifugation at 55,000 rpm, filtered through a 0.45 μm pore filter, and finally, stored in Dulbecco’s phosphate-buffered saline (DPBS) at pH 7.4 before use.
Wang G., Griffin J.I., Inturi S., Brenneman B., Banda N.K., Holers V.M., Moghimi S.M, & Simberg D. (2017). In Vitro and In Vivo Differences in Murine Third Complement Component (C3) Opsonization and Macrophage/Leukocyte Responses to Antibody-Functionalized Iron Oxide Nanoworms. Frontiers in Immunology, 8, 151.
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2

Fluorescent Nanoparticle Bioconjugation

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0.5 mg of the fluorophore-conjugated particles were then dispersed in 80 μL of ethanol and were mixed with a solution of (180 μL) of the heterofunctional linker maleimide-PEG-succinimidyl valerate (MAL-PEG-SVA, MW = 3400, Laysan Bio Inc.) in ethanol (5 mg/mL) following previous methods.17 (link) The PEG-nanoparticle mixture was incubated overnight at room temperature under mild shaking and free from light, to prevent bleaching of the fluorophore. Any unbound PEG was removed with a triplicate wash in ethanol and centrifuged (15,000 rpm, 10 min). The particles were then dispersed in ethanol and stored in dark.
Vijayakumar S., Nasr S.H., Davis J.E., Wang E., Zuidema J.M., Lu Y.S., Lo Y.H., Sicklick J.K., Sailor M.J, & Ray P. (2022). Anti-KIT DNA Aptamer-conjugated Porous Silicon Nanoparticles for the Targeted Detection of Gastrointestinal Stromal Tumors. Nanoscale, 14(47), 17700-17713.
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3

Functionalization of Amyloid-beta Peptide

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N-terminus azide modified Aβ42 [K(N3)-Aβ42] peptide was purchased from GenicBio Limited (Shanghai, China). MAL-PEG-SVA (M.wt 3400 g/mol) and mPEG-SVA (M.wt 2000 g/mol) were purchased from Laysan Bio (Arab, AL), Tris-(2-Carboxyethyl) phosphine-HCl (TCEP-HCl) was purchased from Hampton Research Inc. (CA, USA). NHS-PEG4-DBCO, and Cystamine, HCl were purchased from Sigma-Aldrich, USA. 1-(3-aminopropyl) silatrane (APS) were synthesized as described in ref23 . All other commercial solvents were purchased from Sigma-Aldrich (MO, USA). Water used in all the experiments is deionized (DI) water obtained from AquaMAX-ultra (APS water service corporation, USA) water purification system.
Maity S, & Lyubchenko Y.L. (2019). Force Clamp Approach for Characterization of Nano-assembly in Amyloid beta 42 Dimer. Nanoscale, 11(25), 12259-12265.
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4

Cysteine-labeled Aβ(14-23) Peptide Synthesis

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The FNA- Aβ(14–23) dimer was synthesized as described in our previous paper (Figure S1).28 HPLC purified cysteine-labeled HQKLVFFAED Aβ(14–23) was obtained from Peptide 2.0 (VA, USA). Cyanine 3-Maleimide (Cy3-MAL) was purchased from Lumiprobe Corporation (Florida, USA). MAL-PEG-SVA (M.wt 3400 g/mol) and mPEG-SVA (M.wt 2000 g/mol) were from Laysan Bio (Arab, AL), Tris-(2-Carboxyethyl) phosphine, Hydrochloride (TCEP) was from Hampton Research Inc. (CA, USA), β-mercaptoethanol and 1,1,1,3,3,3 Hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich, USA. Maleimide-polyethylene glycol-silatrane (MAS) and 1-(3-aminopropyl) silatrane (APS) were synthesized as described in ref 33 . All other commercial solvents were purchased from Sigma-Aldrich.
Maity S., Viazovkina E., Gall A, & Lyubchenko Y.L. (2017). Single-molecule probing of amyloid nano-ensembles using the polymer nanoarray approach. Physical chemistry chemical physics : PCCP, 19(25), 16387-16394.
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5

Amyloid-β Peptide Modification and Conjugation

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Peptides [CHQKLVFFAED and CHQKLVFFAED-YNGK-HQKLVFFAED] were purchased from Peptide 2.0 Inc. (VA, USA). N-hydroxysuccinimide (NHS) and maleimide (MAL) functionalized Cyanine3 dyes (Cyanine3-NHS and Cyanine3-MAL) were purchased from Lumiprobe Corporation (Florida, USA). MAL-PEG-SVA (M.W 3400 g/mol) and mPEG-SVA (M.W 2000 g/mol) were from Laysan Bio (Arab, AL), Tris-(2-Carboxyethyl) phosphine, Hydrochloride (TCEP) was from Hampton Research Inc. (CA, USA), β-mercaptoethanol and 1,1,1,3,3,3 Hexafluoroisopropanol (HFIP) were purchased from Sigma-Aldrich, USA. The synthesis of maleimide-polyethylene glycol-silatrane (MAS) and 1-(3-aminopropyl) silatrane (APS) was described in our previous publication40 (link).
Maity S., Hashemi M, & Lyubchenko Y.L. (2017). Nano-assembly of amyloid β peptide: role of the hairpin fold. Scientific Reports, 7, 2344.
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