Erp 10

Manufactured by Eicom

Sourced in United States, Japan

The ERP-10 is a programmable electronic control unit designed for laboratory equipment. It features a high-performance microprocessor, multiple input/output channels, and advanced control algorithms to precisely regulate various system parameters. The core function of the ERP-10 is to provide reliable and accurate control capabilities for laboratory-scale applications.

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Lab products found in correlation

Esp 32, by Eicom (2 mentions) Kds101, by KD Scientific (1 mentions) Peg 4, by Eicom (1 mentions)

3 protocols using erp 10

In vivo Hippocampal Aβ Monitoring in APP/PS1 Mice

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In vivo microdialysis experiments used to assess brain interstitial fluid (ISF) Aβ levels from awake and freely moving APP/PS1 mice at the age of 3 months, 2 weeks after BCAS or sham operation, were developed with a modification of a previously described method^{26 (link)}. The guide cannula was stereotactically inserted into the hippocampus using the following coordinates: anterior-posterior −2.8 mm, medial-lateral ± 0.5 mm, dorsal-ventral −1.3 mm, from the bregma.
The probes used for in vivo microdialysis were equipped with 1000 kDa cut-off membrane, and connected to push (KDS101, Kd Scientific, Holliston, MA, USA) and pull pumps (ERP-10, Eicom, San Diego, CA, USA). The probes were manually inserted through the guide cannula into the target region. After probe insertion, mice were placed into cages designed to allow unrestricted movement of the animal without probe assembly tangling. To measure Aβ₄₀ and Aβ₄₂, microdialysis probes had a constant flow rate of 1.3 μl/min. Microdialysis samples were collected hourly using a refrigerated fraction collector.

Bannai T., Mano T., Chen X., Ohtomo G., Ohtomo R., Tsuchida T., Koshi-Mano K., Hashimoto T., Okazawa H., Iwatsubo T., Tsuji S., Toda T, & Iwata A. (2019). Chronic cerebral hypoperfusion shifts the equilibrium of amyloid β oligomers to aggregation-prone species with higher molecular weight. Scientific Reports, 9, 2827.

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In vivo Microdialysis of Brain Tau

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In vivo microdialysis sampling of brain interstitial fluid (ISF) tau was performed as described previously^{7 ,21 (link),22 (link)}. The microdialysis probe had a 4 mm shaft with a 3.0 mm, 1000 kDa molecular weight cutoff (MWCO) polyethylene membrane (PEP-4-03, Eicom, Japan). Before use, the probe was conditioned by briefly dipping it in ethanol, and then washed with an artificial cerebrospinal fluid (aCSF) perfusion buffer (in mM: 122 NaCl, 1.3 CaCl₂, 1.2 MgCl₂, 3.0 KH₂PO₄, 25.0 NaHCO₃) that was filtered through a 0.2 μm pore-size membrane. The preconditioned probe's outlet and inlet were connected to a peristaltic pump (ERP-10, Eicom) and a microsyringe pump (ESP-32, Eicom), respectively, using fluorinated ethylene propylene (FEP) tubing (φ 250 μm i.d.).
Probe implantation was performed as previously described^{22 (link)}, with slight modifications. Briefly, the animals were anesthetized with isoflurane, while a guide cannula (PEG-4, Eicom) was stereotactically implanted in the hippocampus (bregma -3.1 mm, -2.5 mm lateral to midline, -1.0 mm ventral to dura at a 12° angle). Three days after the implantation of the guide cannula, the mice were placed in a standard microdialysis cage and a probe was inserted through the guide. ISF samples were collected at a flow rate of 0.5 μl/min.

Takeda S., Commins C., DeVos S.L., Nobuhara C.K., Wegmann S., Roe A.D., Costantino I., Fan Z., Nicholls S.B., Sherman A.E., Trisini Lipsanopoulos A.T., Scherzer C.R., Carlson G.A., Pitstick R., Peskind E.R., Raskind M.A., Li G., Montine T.J., Frosch M.P, & Hyman B.T. (2016). Seed-competent HMW tau species accumulates in the cerebrospinal fluid of Alzheimer's disease mouse model and human patients. Annals of neurology, 80(3), 355-367.

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Long-term CSF Collection in Freely Moving Mice

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Four days after the CDP implantation, the mice were tethered with the sensor-integrated balance arm and placed in the movement-response caging system (MD-1409, BASi, USA) as previously described [18 (link)]. The sensor detects the animal’s rotation and turns the cage in the opposite direction, allowing unrestricted movement by the animals without applying pressure to the probe assembly. The CSF collection tubing (M025V-100, Eicom, Japan) from the CDP was connected to a roller pump (ERP-10, Eicom, Japan) or syringe pump (ESP-32, Eicom, Japan) to withdraw CSF at a constant flow rate. Maintaining the constant flow of CSF prevents healing or closing the AOM and enables long-term collection (e.g., four weeks). The movement-response caging system was placed in the sound-attenuating box (Natsume Seisakusho, Osaka, Japan) to control the environmental conditions. The mice were maintained at room temperature (25 °C ± 2 °C) under a standard 12-h/12-h light-dark cycle, with free access to water and food. The mice’s locomotor activity during the experiment was measured by an infrared light beam crossing system as described below.

Nakajima T., Takeda S., Ito Y., Oyama A., Takami Y., Takeya Y., Yamamoto K., Sugimoto K., Shimizu H., Shimamura M., Rakugi H, & Morishita R. (2022). A novel chronic dural port platform for continuous collection of cerebrospinal fluid and intrathecal drug delivery in free-moving mice. Fluids and Barriers of the CNS, 19, 31.

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