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Cellulose ester membrane

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Cellulose ester membrane is a type of laboratory filtration equipment. It is a thin, porous membrane made from cellulose esters. The membrane is designed to separate and filter materials based on their size and molecular weight.

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Lab products found in correlation

Slanetz and bartley agar medium, by Biolife (2 mentions) Legionella bcye growth supplement, by Thermo Fisher Scientific (1 mentions) Legionella cye agar base, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mixed cellulose esters membrane Cellulose mixed ester (CME) filter membranes Multiscreen HA mixed cellulose ester membrane plates Mixed cellulose ester membrane Mixed cellulose ester membrane filter Cellulose membrane

18 protocols using cellulose ester membrane

1

Isolation and Identification of Pseudomonas aeruginosa

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Isolation and identification of Pseudomonas aeruginosa was performed according to the standardized procedure UNI EN ISO 16266:2008 [24 ]. Briefly, 100 mL of each water sample was filtered with a cellulose ester membrane (0.45 μm porosity, 47 mm diameter; Millipore, Billerica, MA, USA), which was then placed onto a Pseudomonas Agar with Pseudomonas CN Supplement (PACN) (Oxoid, Basingstoke, UK) plate.
PACN plates were incubated at 35 ± 1°C for 44 ± 4 h before the counting of colonies. Blue/green pyocyanin-producing colonies were counted as confirmed P. aeruginosa according to UNI EN ISO 16266:2008 [24 ]. Fluorescent non-pyocyanin-producing or reddish brown colonies were recorded as presumptive P. aeruginosa and subjected to confirmation tests according to UNI EN ISO 16266:2008 [24 ].
Schiavano G.F., Carloni E., Andreoni F., Magi S., Chironna M., Brandi G, & Amagliani G. (2017). Prevalence and antibiotic resistance of Pseudomonas aeruginosa in water samples in central Italy and molecular characterization of oprD in imipenem resistant isolates. PLoS ONE, 12(12), e0189172.
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2

Quantifying Pseudomonas aeruginosa in Water Samples

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Each sample (250 mL) was filtered through a cellulose ester membrane (47 mm Ø and 0.45-µm pore size; Millipore, Milan, Italy). The membrane was placed on a plate containing Pseudomonas selective agar supplemented with cetrimide (0.20 g) and nalidixic acid (15 mg) (Microbiol, Cagliari, Italy) and incubated at 36 ± 2 °C for 44 ± 4 h. Blue-green pyocyanin producing colonies were directly confirmed to be P. aeruginosa [58 ]. The results were reported as CFU/250 mL.
Montagna M.T., De Giglio O., Calia C., Pousis C., Triggiano F., Murgolo S., De Ceglie C., Bagordo F., Apollonio F., Diella G., Narracci M., Acquaviva M.I., Ferraro G.B., Mancini P., Veneri C., Brigida S., Grassi T., De Donno A., Di Iaconi C., Caputo M.C., Cavallo R.A., La Rosa G, & Mascolo G. (2020). Microbiological and Chemical Assessment of Wastewater Discharged by Infiltration Trenches in Fractured and Karstified Limestone (SCA.Re.S. Project 2019–2020). Pathogens, 9(12), 1010.
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3

Salmonella Detection in Water Samples

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A 1 L subsample of each water sample was filtered by a cellulose ester membrane (142 mm Ø and 0.45 μm pore size; Millipore, Milan, Italy). This membrane was placed in 100 ml sterile 0.1 % (w/v) peptone water (Thermo Scientific Oxoid, Milan, Italy) and homogenized for 1 min. Subsequently, an aliquot of the homogenized material was mixed with a selective enrichment medium, consisting of selenite cystine broth (Biolife Italiana srl, Milan, Italy). After incubation for 24 h at 35 °C, it was subcultured on two agar plates, brilliant green and xylose lysine deoxycholate (Becton Dickinson, Heidelberg, Germany), and incubated for another 24 h at 35 °C. From each plate, at least one colony suspected of being Salmonella was inoculated on triple sugar iron and lysine iron agar (Biolife Italiana srl, Milan, Italy), incubated for 24 h at 35 °C, and typed via specific serological tests (Standard Methods 9260D ).
De Giglio O., Barbuti G., Trerotoli P., Brigida S., Calabrese A., Di Vittorio G., Lovero G., Caggiano G., Uricchio V.F, & Montagna M.T. (2016). Microbiological and hydrogeological assessment of groundwater in southern Italy. Environmental Monitoring and Assessment, 188(11), 638.
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4

Quantification of E. coli and Total Coliforms

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A 100-ml aliquot of the water sample was filtered through a cellulose ester membrane (47 mm Ø and 0.45 μm-pore size; Millipore, Milan, Italy), placed on Tergitol 7-Triphenyltetrazolium chloride agar (Biolife Italiana srl, Milan, Italy) and incubated at 36 ± 2 °C for 24 ± 2 h. If no typical colonies were present, then the samples were incubated for an additional 24 ± 2 h. Lactose-positive colonies were subcultured onto a tryptone tryptophan medium (Sigma-Aldrich, St. Louis, MO, USA) and incubated at 37 ± 1 °C for 24 ± 2 h. If the resulting colonies were oxidase negative and indole positive, then they were assumed to be EC, while if they were oxidase negative, they were assumed to be TC (EN ISO 9308-1:2000 ).
De Giglio O., Barbuti G., Trerotoli P., Brigida S., Calabrese A., Di Vittorio G., Lovero G., Caggiano G., Uricchio V.F, & Montagna M.T. (2016). Microbiological and hydrogeological assessment of groundwater in southern Italy. Environmental Monitoring and Assessment, 188(11), 638.
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5

Pseudomonas Isolation from Water Samples

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A 250-ml aliquot of the water sample was filtered through a cellulose ester membrane (47 mm Ø and 0.45 μm pore size, Millipore, Milan, Italy), placed over a Pseudomonas agar plate added to cetrimide and nalidix acid (Biolife Italiana srl, Milan, Italy), and incubated at 36 ± 1 °C for 48 h (EN ISO 12780:2002 ).
De Giglio O., Barbuti G., Trerotoli P., Brigida S., Calabrese A., Di Vittorio G., Lovero G., Caggiano G., Uricchio V.F, & Montagna M.T. (2016). Microbiological and hydrogeological assessment of groundwater in southern Italy. Environmental Monitoring and Assessment, 188(11), 638.
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6

Enumeration of Enterococci in Water

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A 100-ml aliquot of each water sample was filtered through a cellulose ester membrane (47 mm Ø and 0.45 μm pore size; Millipore, Milan, Italy). The membrane was placed over a Slanetz and Bartley agar medium (Biolife Italiana srl, Milan, Italy) and incubated at 36 ± 1 °C for 48 h. The colonies ranged in color from pink to dark red and brown, but only catalase and esculin hydrolysis positive ones were considered to be ENT (EN ISO 7899-2: 2003 ).
De Giglio O., Barbuti G., Trerotoli P., Brigida S., Calabrese A., Di Vittorio G., Lovero G., Caggiano G., Uricchio V.F, & Montagna M.T. (2016). Microbiological and hydrogeological assessment of groundwater in southern Italy. Environmental Monitoring and Assessment, 188(11), 638.
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7

Enterococci Quantification in Water Samples

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A 100-mL aliquot of each sample was filtered through a cellulose ester membrane (47 mm Ø and 0.45-µm pore size; Millipore, Milan, Italy). The membrane was placed over a Slanetz and Bartley agar medium (Biolife Italiana srl, Milan, Italy) and incubated at 36 ± 1 °C for 48 h. The colonies ranged in color from pink to dark red and brown, but only catalase- and esculin hydrolysis-positive colonies were considered to be enterococci [57 ]. The results were reported as CFU/100 mL.
Montagna M.T., De Giglio O., Calia C., Pousis C., Triggiano F., Murgolo S., De Ceglie C., Bagordo F., Apollonio F., Diella G., Narracci M., Acquaviva M.I., Ferraro G.B., Mancini P., Veneri C., Brigida S., Grassi T., De Donno A., Di Iaconi C., Caputo M.C., Cavallo R.A., La Rosa G, & Mascolo G. (2020). Microbiological and Chemical Assessment of Wastewater Discharged by Infiltration Trenches in Fractured and Karstified Limestone (SCA.Re.S. Project 2019–2020). Pathogens, 9(12), 1010.
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8

Evaluating Ice Sample Microbial Safety

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After melting at room temperature, the ice samples were evaluated for the mandatory parameters provided for in Decree No. 31/2001 relating to E. coli, coliforms and Enterococci, and some accessory parameters relating to Staphylococcus aureus, P. aeruginosa and fungi. Each sample was filtered through a cellulose ester membrane with a diameter of 47 mm and a pore size of 0.45 μm (Millipore, Milan, Italy). The samples were considered compliant when E. coli, coliforms and Enterococci were absent in 100 mL of each melted ice sample.
Caggiano G., Marcotrigiano V., Trerotoli P., Diella G., Rutigliano S., Apollonio F., Marzella A., Triggiano F., Gramegna M., Lagravinese D., Sorrenti G.T., Magarelli P., Moscato U, & Montagna M.T. (2020). Food Hygiene Surveillance in Italy: Is Food Ice a Public Health Risk?. International Journal of Environmental Research and Public Health, 17(7), 2408.
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9

Microbiological and Protozoan Analysis of Irrigation Water

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For the microbiological analysis by means of the chromogenic substrate technique (APHA, 2005) , 21 samples of water, one per property (from property 1 to 21), were collected from the irrigation tap of the vegetable gardens, as recommended by the protocol in Brazil (BRASIL, 2013) .
For T. gondii, Cryptosporidium spp. and G. intestinalis detection, a total of 10 samples (from property 12 to 21) of 10 mL were collected in clean plastic bottles from irrigation tap. The water was filtered through a cellulose ester membrane with a 47 mm-diameter and 1.2 μm porosity (Millipore®, Billerica, Massachusetts, USA) in a filter holder system using a vacuum pump (4 L/min). After filtration, the material was eluted in 0.1% Tween 80 with the aid of flexible plastic loops (Thermo Fisher Scientific, Massachusetts, USA) (BRANCO et al., 2012) (link). The obtained material was concentrated by centrifugation twice at 1050 x g for 15 min at 4°C. The obtained pellet was stored in microtubes at -20 °C until DNA extraction.
Ferreira F.P., Caldart E.T., Freire R.L., Mitsuka-Breganó R., Freitas F.M., Miura A.C., Mareze M., Martins F.D.C., Urbano M.R., Seifert A.L, & Navarro I.T. (2018). The effect of water source and soil supplementation on parasite contamination in organic vegetable gardens. Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 27(3).
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10

In Vitro Release of Lapatinib from Dialysis Membranes

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The release of LAP from NE was performed by the dialysis method using tubing cellulose membranes with a cutoff size of 14 kDa and a diameter of 21 mm (cellulose ester membrane; Sigma–Aldrich, St Louis, USA). Dialysis bags were filled with 1 mL of formulation, sealed and incubated with 50 mL of PBS (pH 7.4) containing Tween 80 (2 %), at 37 °C, for 24 h, under magnetic stirring at 150 rpm. An aqueous solution of LAP (in PBS containing 2 % Tween 80) was used as a control (concentration 0.46 mg/mL). At 15, 30, 60, 90, 120, 240, 360, and 1440 min, aliquots were withdrawn and LAP concentration was analyzed by HPLC. The same volume was replaced with the receptor liquid (PBS + Tween 80). Values were plotted as cumulative percentage of drug release.
Mendes Miranda S.E., de Alcântara Lemos J., Fernandes R.S., Silva J.D., Ottoni F.M., Townsend D.M., Rubello D., Alves R.J., Cassali G.D., Ferreira L.A, & de Barros A.L. (2020). Enhanced antitumor efficacy of lapachol-loaded nanoemulsion in breast cancer tumor model. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 133, 110936.
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