Il 4 mouse elisa kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The IL-4 Mouse ELISA Kit is a laboratory product used to measure the concentration of Interleukin-4 (IL-4) in mouse samples. It is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the in vitro determination of mouse IL-4 levels.

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8 protocols using il 4 mouse elisa kit

Inflammatory Cytokine Profiling in AD Mice

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The HIP, PFC, and plasma samples, from the wt and 3xTg-AD mice, added with protease inhibitors were also used to measure pro- and anti-inflammatory cytokines using the following ELISA kits: the IL-10 Mouse ELISA Kit, the IL-1β Mouse ELISA Kit, the TNF-α Mouse ELISA Kit, High Sensitivity and the IL-4 Mouse ELISA Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA), following the manufacturer’s instructions.

Cecarini V., Gogoi O., Bonfili L., Veneruso I., Pacinelli G., De Carlo S., Benvenuti F., D’Argenio V., Angeletti M., Cannella N, & Eleuteri A.M. (2022). Modulation of Gut Microbiota and Neuroprotective Effect of a Yeast-Enriched Beer. Nutrients, 14(12), 2380.

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Evaluating Ocular Responses to Topical Treatments

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C57BL/6 mice were randomly divided into four groups (six eyeballs for each group) and treated with different eyedrops, including PBS, FCS (dissolved in PBS buffer), CS (dissolved in acetate–acetic acid buffer), and SDS (2 mg/ml, 5 μl each eye), twice every day for 4 weeks. Then, OCT (Heidelberg, Germany), an Espion E3 machine (Diagnosys, Boxborough, MA), a slit lamp, and a tonometer were used to monitor the changes in corneal thickness, retinal thickness, ERG, and IOP. Last, the eyes and main organs were collected for immunofluorescence staining and histological examination, and eyes were collected for cytokine detection using the TGF-β1 Mouse ELISA Kit (Thermo Fisher Scientific, catalog no. BMS608-4), TNF-α Mouse ELISA Kit (Thermo Fisher Scientific, catalog no. BMS607-3), IL-1β Mouse ELISA Kit (Thermo Fisher Scientific, catalog no. BMS6002), IL-6 Mouse ELISA Kit (Thermo Fisher Scientific, catalog no. BMS603-2), and IL-4 Mouse ELISA Kit (Thermo Fisher Scientific, catalog no. BMS613) and the blood was collected for routine blood biochemistry and blood analysis.

Shen J., Gao H., Chen L., Jiang Y., Li S., Chao Y., Liu N., Wang Y., Wei T., Liu Y., Li J., Chen M., Zhu J., Liang J., Zhou X., Zhang X., Gu P., Chen Q, & Liu Z. (2023). Eyedrop-based macromolecular ophthalmic drug delivery for ocular fundus disease treatment. Science Advances, 9(4), eabq3104.

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Cytokine Profiling of SaEVs

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The titre of TNF-α, IL-6, IFN-γ and IL-17A was measured using Mouse ELISA kits purchased from Invitrogen, Carlsbad, CA. The protocol followed the manufacturer’s instructions. In order to investigate whether the cytotoxicity of SaEVs is associated with IL-1β, the production of this cytokine was determined by Mouse IL-1β ELISA kit (Invitrogen). For the Th2-related cytokines, IL-4 and IL-5 were determined using IL-4 Mouse ELISA kit (Invitrogen) and IL-5 Mouse ELISA kit (Invitrogen), respectively by following the manufacturer’s instructions.

Asano K., Hirose S., Narita K., Subsomwong P., Kawai N., Sukchawalit R, & Nakane A. (2021). Extracellular vesicles from methicillin resistant Staphylococcus aureus stimulate proinflammatory cytokine production and trigger IgE-mediated hypersensitivity. Emerging Microbes & Infections, 10(1), 2000-2009.

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Cytokine Quantification in Splenocytes

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Cytokine levels of IL-4 and IFN-γ in the splenocyte culturing surpernatants were measured using IFN gamma or IL-4 Mouse ELISA Kit (Invitrogen, USA) according to the manufacturer's instructions. The OD_450nm values were read and converted to picograms per milliliter. Concentrations were calculated based on the standard curves generated in each plate.

Yu G., Liang W., Yang Q., Wang J., Wang Y., Zhang T., Zhang X., Fan H., Zhao P., Cao L, & Dong J. (2021). Immune Protective Evaluation Elicited by DNA Vaccination With Neospora caninum Dense Granules Proteins in Mice. Frontiers in Veterinary Science, 8, 638067.

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Quantifying Inflammatory Mediators in Lung Tissues

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Lung tissues were weighed and homogenized in RIPA lysis buffer containing 10 mM PMSF and a protease inhibitor cocktail (1 g/12 μL). Homogenates were ultrasonicated and centrifuged at 12,000 rpm for 10 min at 4 °C, and the supernatants were collected. mCCL6 concentrations in the serum (1:5 dilution), lung tissue (1:100 dilution) and BALF supernatant (1:10 dilution) were detected by a Mouse CCL6/C-C Motif Chemokine 6 ELISA Kit (RAB0557, Sigma-Aldrich). IL4 and IL33 concentrations in lung tissues were detected by an IL-4 Mouse ELISA Kit (BMS613, Invitrogen) and an IL-33 Mouse ELISA Kit (1:20 dilution, BMS6025, Invitrogen), respectively. CCL23 concentrations in the serum of human samples were detected using a Human MPIF-1 ELISA Kit (RAB0065, Sigma-Aldrich). All these above-mentioned detections were performed according to the relevant manufacturer’s instructions.

Du X., Li F., Zhang C., Li N., Huang H., Shao Z., Zhang M., Zhan X., He Y., Ju Z., Li W., Chen Z., Ying S, & Shen H. (2021). Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation. Signal Transduction and Targeted Therapy, 6, 91.

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Quantifying Immune Markers in Mouse Samples

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The mouse serum and BALF were used to assess the levels of IgE, IL-17, and IL-4 using an IgE mouse uncoated ELISA kit with plates (Invitrogen, Carlsbad, CA, USA), an IL-17 mouse ELISA kit (Invitrogen), and an IL-4 mouse ELISA kit (Invitrogen), respectively, according to the protocols recommended by the manufacturer. After the reaction, the absorbance at 450 nm was measured using a microplate reader (SpectraMax 340PC, Molecular Devices), and the concentrations of IgE, IL-17, and IL-4 were calculated according to the corresponding standard curve.

An M., Oh M., Park K.T., Seon K.H., Jo J.E., Lee S.K., Kim J.K., Shin K.S., Koh J.H, & Lim Y.H. (2021). Anti-asthma and antitussive effects of a fermented extract of a mixture of Ramulus mori, Anthriscus sylvestris, and Salvia plebeian. Food Science and Biotechnology, 30(9), 1257-1268.

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Allergic Inflammation Characterization in Mice

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BIS, Mouse anti-dinitrophenyl (DNP) IgE, 2,4-dinitrochlorobenzene (DNCB), glycine, cell proliferation Kit II, 4-Nitrophenyl N-acetyl-β-D-glucosaminide (p-NAG), bovine serum albumin (BSA), ketotifen, formamide, Evans Blue, and o-phthaldialdehyde were purchased from Sigma-Aldrich (ST. Louis, MO, USA). The 2,4-dinitrophenyl-human serum albumin (DNP-HSA) and 2,4-dinitrophenyl-bovine serum albumin (DNP-BSA) were from Biosearch technology. The antibodies specific for p-JNK (Thr183/Tyr185), JNK, p-Erk1/2 (Thr202/Tyr204), Erk1/2, p-P38 (Thr180/Tyr182), P38, p-IκBα (Ser32), IκBα, and normal rabbit IgG were from Cell Signaling Technology (Danvers, MA, USA). The antibodies specific for GAPDH were from Proteintech (Chicago, USA). RIPA and BCA protein assay kits were from Beyotime (Beijing, China). Fetal Bovine Serum (FBS), mouse TNF alpha ELISA kit, mouse IL-4 ELISA kit, and mouse IgE ELISA kit were from Thermo Fisher. Recombinant mouse IL-3 and recombinant mouse SCF/c-kit ligand were from RD systems China. ECL Immobilon Western Chemiluminescent HRP Substrate was from Millipore (Billerica, USA). Compound dexamethasone acetate cream was purchased from CR SAN JIU (Shenzhen, China).

Li G., Wu H., Sun L., Cheng K., Lv Z., Chen K., Qian F, & Li Y. (2022). (-)-α-Bisabolol Alleviates Atopic Dermatitis by Inhibiting MAPK and NF-κB Signaling in Mast Cell. Molecules, 27(13), 3985.

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T Cell Activation and IL-4 Measurement

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Splenic CD4⁺ T cells or naïve CD4⁺ T cells were isolated as described above. Cells were stimulated in vitro with Leukocyte Activation Cocktail with BD GolgiPlug (PMA/ionomycin) (BD Biosciences), Dynabeads Mouse T-Activator CD3/CD28 (Thermo Fisher Scientific), or plate bound anti-TCRβ (10 μg/ml) and anti-CD28 (1 μg/ml) for 12 to 72 hours according to the manufacturer’s instructions. IL-4 level in supernatant was analyzed by mouse IL-4 ELISA kit (Thermo Fisher Scientific).

Misawa T., SoRelle J.A., Choi J.H., Yue T., Wang K.W., McAlpine W., Wang J., Liu A., Tabeta K., Turer E., Evers B., Nair-Gill E., Poddar S., Su L., Ou F., Yu L., Russell J., Ludwig S., Zhan X., Hildebrand S., Li X., Tang M., Murray A.R., Moresco E.M, & Beutler B. (2020). Mutual inhibition between Prkd2 and Bcl6 controls T follicular helper cell differentiation. Science immunology, 5(43), eaaz0085.

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