Enzyme linked immunosorbent assay elisa kits for tnf α

Egg yolk L-phosphatidylethanolamine (EYPE), egg yolk l-phosphatidyl-DLglycerol (EYPG), LPS purified from Escherichia coli O111:B4, were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3,3′-Dipropylthiadicarbocyanine iodide (diSC₃-5), SYTO 9 and propidium iodide (PI) were supplied from Molecular Probes (Eugene, OR, USA). HyClone Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from SeouLin Bioscience (Seoul, Korea). The enzyme‐linked immunosorbent assay (ELISA) kits for TNF‐α were procured from R&D Systems (Minneapolis, MN).

Chemical tanshinone IIA sodium sulfonate (TSS, purity is 99%) was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). The structure of TIIA is shown in Figure 1. Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-6, and IL-1β were obtained from R&D Systems (Minneapolis, MN, USA). Monoclonal β-actin antibody, Escherichia coli endotoxin LPS (O55:B5), Evans blue dye, and all the other reagents were obtained from Sigma-Aldrich Inc. (St. Louis, MO, USA). The purity of all chemical reagents was at least in analytical grade.