Anti demethylated pp2a c mab

The Dulbecco modified Eagle medium (DMEM), optiMEM, trypsin (0.25%), l-glutamine, penicillin, streptomycin, fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Okadaic acid was obtained from Calbiochem (San Diego, CA, USA), and 3-O-Methyl-sphingomyeline (3-OME-SM) was purchased from Biomol (Plymouth Meeting, PA, USA). The anti-phospho-p44/p42 extracellular signal-regulated kinase (ERK) (Thr²⁰²/Tyr²⁰⁴) was purchased from Cell Signaling (Beverly, MA, USA). The anti-phospho-Akt (Ser⁴⁷³) and anti-Akt monoclonal antibodies (mAbs) were purchased from Biovision (Mountain View, CA, USA). The anti-phospho-PP2A-C mAb, and anti-demethylated-PP2A-C mAb were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The anti-α-tubulin mAb was purchased from Neo Markers (Fremont, CA, USA). The horseradish peroxidase conjugated donkey anti-rabbit immunoglobulin G (IgG) and sheep anti-mouse IgG were purchased from Amersham (Buckinghamshire, UK). The Hybond-P polyvinylidene difluoride (PVDF) membrane and enhanced chemiluminescence (ECL) detection reagent were purchased from Amersham. Ketamine was dissolved in 0.1% DMSO, and stored at 4 °C.