Equal amounts of protein were separated in 8 to 10% SDS-polyacrylamide gel and transferred to Immobilon-P polyvinylidene fluoride membrane (Millipore). The membrane was blocked with 5% skimmed milk in PBS-0.1% Tween 20 (PBST) (Sigma-Aldrich) at room temperature and incubated with primary and secondary antibody solutions in 2% skimmed milk in PBST. The antibodies were used in the following dilutions: rabbit polyclonal anti-TCF4 (CeMines) 1:1000, mouse monoclonal anti-E2 (5E11, Icosagen,) 1:5000, mouse monoclonal anti-GAPDH (MAB374, Millipore) 1:4000, horseradish peroxidase-conjugated goat anti-mouse/rabbit IgG (Thermo Scientific) 1:5000. For chemiluminescent reaction, SuperSignal West Femto Maximum Sensitivity Substrate Kit (Thermo Scientific) was used, and the reaction was visualized with ImageQuant LAS4000 camera system (GE Healthcare).
Mouse monoclonal anti gapdh mab374
Mouse monoclonal anti-GAPDH (MAB374) is a laboratory reagent used for the detection and quantification of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) protein in biological samples.
Lab products found in correlation
3 protocols using mouse monoclonal anti gapdh mab374
Western Blot Analysis of Protein Extracts
Equal amounts of protein were separated in 8 to 10% SDS-polyacrylamide gel and transferred to Immobilon-P polyvinylidene fluoride membrane (Millipore). The membrane was blocked with 5% skimmed milk in PBS-0.1% Tween 20 (PBST) (Sigma-Aldrich) at room temperature and incubated with primary and secondary antibody solutions in 2% skimmed milk in PBST. The antibodies were used in the following dilutions: rabbit polyclonal anti-TCF4 (CeMines) 1:1000, mouse monoclonal anti-E2 (5E11, Icosagen,) 1:5000, mouse monoclonal anti-GAPDH (MAB374, Millipore) 1:4000, horseradish peroxidase-conjugated goat anti-mouse/rabbit IgG (Thermo Scientific) 1:5000. For chemiluminescent reaction, SuperSignal West Femto Maximum Sensitivity Substrate Kit (Thermo Scientific) was used, and the reaction was visualized with ImageQuant LAS4000 camera system (GE Healthcare).
Immunoblot Analysis of Neural Markers
Immunohistochemical Staining Protocol
goat anti-mouse IgG-horseradish peroxidase (HRP); goat anti-rabbit IgG-HRP; donkey anti-goat IgG-HRP.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!