Human embryonic kidney (HEK) 293 cells and Mouse Embryonic Fibroblast (MEF) from the American Type Culture Collection were grown as described previously [25 (link)]. Transfections were achieved using jetPRIME DNA Transfection Reagent (Polyplus Transfection, VWR International) following the manufacturer’s directions. Cells were grown in DMEM supplemented with 10% Heat-inactivated Fetal Calf Serum (Atlanta Biologicals, Atlanta, GA) and antibiotic/antimyotic solution (Sigma-Aldrich, St. Louis, MO) at 37°C with high humidity and 5% CO2 environment.
Jetprime dna transfection reagent
Manufactured by Avantor
Sourced in Canada
JetPRIME DNA Transfection Reagent is a laboratory product designed for efficient DNA transfection into a variety of mammalian cell lines. It facilitates the delivery of DNA into cells, enabling gene expression studies and other related applications.
Automatically generated - may contain errors
2 protocols using jetprime dna transfection reagent
1
HEK293 and MEF Cell Culture Protocol
2
Mitochondrial Targeting of Calpain-1 in Cardiomyocytes
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The full coding region of human capn1 cDNA was recovered from pCMV6-XL5 containing human capn1 (OriGene, Rockville, MD) and inserted into pCMV/myc/mito, which introduced the mitochondrial signal peptide (Life Technologies Inc., Burlington, ON, Canada). The resulting plasmid pCMV/myc/mito-capn1 expresses myc-tagged capn1 selectively in mitochondria. Rat cardiomyocyte-like H9c2 cells were transfected with pCMV/myc/mito-capn1 or pCMV/myc/mito as a control by using the jetPRIME DNA transfection reagent (VWR International, Mississauga, ON, Canada) according to the manufacturer’s instructions.
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